Protocols   
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Library construction (CSHL)      Solutions 

 

Library construction pOT (WUGSC)

 

Life technologies plasmid prep

 

Microwave plasmid prep

 

ThermoMAX prep

 

MegaBace PCR prep from archive source

SPRI plasmid purification protocol

 

MegaBace ET terminator reaction protocol

Big Dye terminator sequencing reactions (25%)

 

Big Dye terminator sequencing reactions (6.25%)

 

Big Dye terminator precipitation reactions

 

Dyenamic direct dye primer manual sequencing reactions

 

 

Basic finishing strategy

 

Finishing reactions

 

Finishing standards

 

consed notes

 

pZero2.1 vector notes