How to proceed from a genetic position to cloning your gene:

If you have determined where your gene maps, either by scoring molecular or visible markers, you can use these tables to identify BAC clones in the corresponding regions. If the region appears to have been sequenced, it is important to confirm that the markers you are using are actually contained within the sequenced clones. This can be accomplished by looking for sequence matches or by performing hybridization experiments.

If the region that corresponds to your gene has not been sequenced, you can get a rough estimate of how long you will need to wait for the sequence to appear by looking at the size of the gaps and consulting the web site of the relevant AGI lab. Note: In a few cases, there are particularly difficult regions for which no contig exists (noted with "? BACs" in the column labeled "Gaps and tiling paths").

If you decide to "walk" in a region that has not been sequenced we suggest the following. First, contact the relevant AGI lab to inquire about their most current mapping status and sequencing queue. Second, we recommend using a consensus of the hybridization and fingerprint contig information. A table of BAC clone names and the fingerprint contig into which they fall is provided in the fingerprint database at this link, while the hybridization database can be searched directly at this link. The arrangement of clones within individual contigs can be determined by searching the fingerprint database directly. Please note that these are putative contigs, and you will have to confirm that they actually are located near your gene.

How to place a DNA sequence on the genetic map:

If you have cloned a piece of DNA and you would like to place it on the genetic map, you can do the following: 1) sequence a portion of your clone and BLAST that sequence against the Arabidopsis sequence database at AtDB to determine if the clone lies on a sequenced BAC or BACend, or 2) hybridize the fragment to the BAC filters available at the Arabidopsis stock center. In either case, search the fingerprint database or the hybridization database for the clones identified, and use the tables presented here to determine an approximate map location for that contig. All major fingerprint contigs have been placed on these tables.