The genome of the fission yeast Schizosaccharomyces pombe consists of about
15 million bases organized into 3 chromosomes. Cold Spring Harbor Laboratory has had a
long term committment to the characterization of this genome. This project began in the
labs of David Beach and Tom Marr who developed a high resolution physical map of the S.
pombe genome. This consists of a minimal tiling path and a larger, more redundant set
of mapped cosmids which comprise the genome (Mizutami, et al.). Another map was developed
independently at the same time (Hohiesel, et al., 1993). The Mizutami, et al. map was the
basis for a pilot sequencing project initiated in the Lita Annenberg Hazen Genome Center
at Cold Spring Harbor. This project has focused on an area near the telomere of chromosome
II. Another sequencing project has
been started on chromosome I by Bart Barrell and collegues at the Sanger Center. This
project has produced a large amount of S. pombe genome sequence.
These web pages summarize the S. pombe sequencing project in our lab at Cold
Spring Harbor and provide links to other sites of interest to S. pombe workers.
Our results are presented in several forms, graphical and text base. They include not only
the sequence itself but relevent mapping information showing the region under study and
preliminary ananlysis of the regions that have been sequenced. Since the annotation of
genomic sequence is an ongoing exercise rather than a project with a fixed end point, we
welcome suggestions from the S. pombe community or comments that we may add to
the annotations about genes in which they may have an interest that we have sequenced.
Comments can be sent to mccombie@cshl.org.