Analysis of overlapping sequences

A comparison of the independently generated sequence of two overlapping clones provides one means by which the accuracy of the sequence data can be judged. We have undertaken such an analysis and present those findings here.


Left Clone Range Right Clone Range Size of Overlap # of discrepancies
T15P10 in finishing at ABI F6N15 1 - ??? detected, but unsized  
F6N15 94344 - 95643 F5I10 110607 - 111906 1300 0
F5I10   F6N23   none detected 0
F6N23   F15P23 in library not checked  
F15P23 in library T18A10   not checked  
T18A10   spanner in library    
spanner in library F2N1      
F2N1 1 - 900 F3D13 34360 - 35259 900 0
F3D13 1 - 200 F11O4 72673 - 72872 200 0
F11O4 1 - 200 T15B16 90397 - 90596 200 0
T15B16 1 - 14900+ T7B11 in finishing at CSHL detected, but unsized  
T7B11 in finishing at CSHL T10M13 1 - 2900+ detected, but unsized  
T10M13 94204 - 105654 T2H3 37239 - 48689 11451 0
T2H3 1 - 200 T14P8 94337 - 94536 200 0
T14P8 1 - 13436 T10P11 100131 - 113566 13436 0
T10P11 1 - 8183 T5J8 1 - 8183 8183 0
T5J8 81518 - 98527 T4I9 81904 - 98913 17010 0
T4I9 1 - 16115 F4C21 1 - 16115 16115 0
F4C21 129598 -136335 F9H3 88782 - 95519 6738 0
F9H3 1 - 31783 T5L23 1 - 31783 31783 0
T5L23   T5H22 1 - ??? none detected
gap estimate - 3 bp
 
T5H22 69553 - 69752 T7M24 1 - 200 200 0
T7M24 37412 - 74475 T25H8 in finishing at WashU detected, but unsized  
T25H8 in finising at WashU T24M8 ??? - 68251 detected, but unsized  
T24M8 1 - 200 T24H24 88649 - 88848 200 0
T24H24 1 - 200 T27D20 80247 - 80446 200 0
T27D20 1 - 629 T19B17 105202 - 105830 629 0
T19B17 1 - 9513 T26N6 1 - 9514 9513 1
T26N6 61084 - 99384 F4H6 1 - 38301 38301 2
F4H6 ??? - 103632 T19J18 in finishing at WashU detected, but unsized  
T19J18 in finishing at WashU T4B21 in finishing at WashU detected, but unsized  
T4B21 in finishing at WashU T1J1 in production detected, but unsized  
T1J1 in production T14O23 in production not checked  
F21I2   F6H8   not checked  
F6H8   T1J24   not checked  
F14G16 in production F28D6 in production not checked  
F28D6 in production T3E15 in production not checked  
T3E15 in production F10A2 in production not checked  
F10A2 in production T6L9 in production not checked  
T6L9 in production F5K24 in production not checked  
F5K24 in finishing T24G23 in finishing detected, but unsized  
T24G23 submitted F1K3 submitted 200 0
F1K3 in production T17A2 in production not checked  
T17A2 in production T13D4 in production not checked  
T13D4 108584 - 110826 F9M13 99402 - 101644 2243 0
F9M13 in production T12G13 ESSA III not checked  
T12G13 ESSA III T28D5 ESSA III not checked  
           
           
T15F16 1 - 11919 T3F12 95265 - 107183 11919 0
T3F12 1 - ??? T10G4 ESSA III not checked  
T10G4 ESSA III T3H13   not checked  
T3H13 in finishing at WashU F23J3 1 - ??? detected, but unsized  
F23J3 47283 - 93489 T8A17 36877 - 83078 46202 5
T8A17 1 - ??? T30A10 in finishing at CSHL not checked  
T30A10 in finishing at CSHL T15G18 in finishing at CSHL not checked  
T15G18 in finishing at CSHL T25P22 in finishing at CSHL detected, but unsized  
T25P22 in finishing at CSHL T24H23 ESSA III not checked  
T24H23 ESSA III T17F16 in production at WashU not checked  
T17F16 in production at WashU T9A4   detected, but unsized  
T9A4   F2P19 ESSA III not checked  
F2P19 ESSA III F3H7 WashU not checked  
F3H7 in finishing at WashU T12H20 69459 - 69658    
T12H20 1 - 200 F8M12 103474 - 103673 200 0
F8M12 1 - 200 F2P3 74207 - 74406 200 0
F6A4 1 - 17452 T11H3 1 - 17452 17452 0
T11H3 67404 - 91916 F21J6 1 - 24515 24513 2
F21J6 94219 - 110680 F18G18 1 - 16462 16462 0
F18G18 84280 - 110469 T14C9 1 - 26191 26191 1
T14C9   F18A17   none detected  
           
           
           
           
T1N24   T19G15   detected, but unsized  
T19G15 11248 - 77036 F9D12 1 - 65786 65786 5
F21E10   F2P16   none detected  
F2P16   T21B4   none detected  
T21B4 1 - 23913 F21A20 1 - 23913 23913 0
F7N22   T25C13      
T25C13   T26D22      
T13L16 78137 - 83131 T17A5 1 - 4995 4995 0
           
TOTAL       372644 15