Gene F5I10.21
Putative Identification cytochrome P450
Position 49610 - 51437, from the initial methionine to the termination codon
Strand -
EST match H76762, N95869, T04172, T46372, T46373, and Z25705
Database match cytochrome P450 CYP86A1 and a segment downstream of frohA, Y09581

 

CDS:  The table below lists the coordinates of the exons for F5I10.21

Exon

Range

1 51016 - 51437
2 49610 - 50849

 

Protein sequence of F5I10.21

MDVSNTMLLVAVVAAYWLWFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENLRAC
GGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIF
NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLIL
RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLGL
EVSLSRSLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFMKKKDQSYSETFLRHV
ALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEFD
EVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKST
WGEDCLEFKPERWISPDDGKFVNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRH
RLTVAPGHKVEQKMSLTLFMKNGLLVNVHKRDLEVMMKSLVPKERNDVVVLNGKCNGGIG
EGVAVNAAVAVAV

 

Comparison of F5I10.21 and the cyp86 gene product. Although the sequence of Y09581 downstream of frohA is very similar to the two proteins presented, no consensus splice sites were identified that would produce an mRNA with an open reading frame.

           1                                                         60
 F5I10.21  ~~~MDVSNTMLLVAVVAAYWLWFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENL 
AtCYP86A1  MEALNSILTGYAVAALSVYALWFYFLSRRLTGPKVLPFVGSLPYLIANRSRIHDWIADNL 

           61                                                       120
 F5I10.21  RACGGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQ 
AtCYP86A1  RATGGTYQTCTMVIPFVAKAQGFYTVTCHPKNVEHILKTRFDNYPKGPMWRAAFHDLLGQ 

           121                                                      180
 F5I10.21  GIFNSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQD 
AtCYP86A1  GIFNSDGDTWLMQRKTAALEFTTRTLRQAMARWVNGTIKNRLWLILDRAVQNNKPVDLQD 

           181                                                      240
 F5I10.21  LILRLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLG 
AtCYP86A1  LFLRLTFDNICGLTFGKDPETLSLDLPDNPFSVAFDTATEATLKRLLYTGFLWRIQKAMG 

           241                                                      300
 F5I10.21  LGLEVSLSRSLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFMKKKDQS...YSE 
AtCYP86A1  IGSEDKLKKSLEVVETYMNDAIDARKN..........SPSDDLLSRFLKKRDVNGNVLPT 

           301                                                      360
 F5I10.21  TFLRHVALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSVLIETRGTDVSSWTA 
AtCYP86A1  DVLQRIALNFVLAGRDTSSVALSWFFWLVMNNREVETKIVNELSMVLKETRGNDQEKWTE 

           361                                                      420
 F5I10.21  EPLEFDEVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAA 
AtCYP86A1  EPLEFDEADRLVYLKAALAETLRLYPSVPQDFKYVVEHDVLPDGTFVPRGSTVTYSIYSI 

           421                                                      480
 F5I10.21  GRMKSTWGEDCLEFKPERWISPDDGKF.VNHDQYRFVAFNAGPRICLGKDLAYLQMKTIA 
AtCYP86A1  GRMKTIWGEDCLEFRPERWLTADGERFETPKDGYKFVAFNAGPRTCLGKDLAYNQMKSVA 

           481                                                      540
 F5I10.21  AAVLLRHRLTVAPGHKVEQKMSLTLFMKNGLLVNVHKRDLEVMMKSLVPKERNDVVVLNG 
AtCYP86A1  SAVLLRYRVFPVPGHRVEQKMSLTLFMKNGLRVYLQPRGEVLA*~~~~~~~~~~~~~~~~ 

           541               561
 F5I10.21  KCNGGIGEGVAVNAAVAVAV*
AtCYP86A1  ~~~~~~~~~~~~~~~~~~~~~

 

written 8 Jan 98
Larry Parnell