This page contains the annotation of F6P23.1 and F6P23.2, tetracycline transporter-like genes.

 

Gene F6P23.1
Putative Identification tetracycline transporter-like protein
Position 1 to 1682, from within the CDS to the termination codon
Strand +
EST match W43055
Database match Mouse mRNA for tetracycline transporter-like protein (D88315)

 

CDS:  The table below lists the coordinates for the exons of F6P23.1 and which exon predicting program selected the 5' and 3' termini (Gr = GRAIL, M = MZEF). EST W43055 defines several exon termini (designated as EST).

Exon Range 5' 3'
1 1 to 12    
2 97 to 129 M,Gr M,Gr
3 283 to 336 M,Gr M
4 419 to 460 M,Gr EST,M
5 1027 to 1105 EST,Gr EST,Gr
6 1271 to 1341 EST EST
7 1437 to 1532 EST  

CDS of F6P23.1 from within the CDS to the termination codon

GAATTCTTCAACGCGACTTGTCTTAGTGTTGCATGGTCACCATGGGTTCCTTATGCCATG
ACCATGCTTGTACCCGGAGCCATGTTTGTGATGCCATCTGTTTGTGGTATTGCGTCAAGA
CAAGTTGGATCCAGTGAACAGGGGAAGGTCCAAGGATGTATATCCGGGGTAAGGGCTTTT
GCTCAAGTTGTGGCTCCATTTGTATATAGTCCATTGACAGCTCTATTTCTCTCCGAAAAT
GCACCATTCTATTTCCCTGGATTCAGCATTCTATGCATCGCCATCTCTTTGATGATCGGA
TTCCTTCAGAGTCTTCTGATAAAAGATCATCCTTCTTTGCCCTTAGTGAACATAACAATC
AACAACCCTTCAAAAGCGGAAGCTTGA

 

Protein translation:

EFFNATCLSVAWSPWVPYAMTMLVPGAMFVMPSVCGIASRQVGSSEQGKVQGCISGVRAF
AQVVAPFVYSPLTALFLSENAPFYFPGFSILCIAISLMIGFLQSLLIKDHPSLPLVNITI
NNPSKAEA*


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Gene F6P23.2
Putative Identification tetracycline transporter-like protein
Position 3722 to 8371, from initial methionine to termination codon
Strand +
EST match H76334
Database match Mouse mRNA for tetracycline transporter-like protein (D88315)

 

CDS:  The table below lists the coordinates for each of the F6P23.2 exons and which exon predicting program selected the 5' and 3' termini (Gr = GRAIL, M = MZEF). Termini selected by EST H76334 are designated by EST.

Exon Range 5' 3'
1 3782 to 3952 EST,Gr EST
2 4726 to 4837 EST EST
3 6023 to 6093 EST EST
4 6237 to 6359 M,Gr M,Gr
5 6469 to 6708 M,Gr  
6 6965 to 7051 M,Gr M
7 7167 to 7247 M M
8 7351 to 7383 M,Gr  
9 7466 to 7519 M,Gr M,Gr
10 7809 to 7850 M,Gr M,Gr
11 7953 to 8031 M,Gr M,Gr
12 8122 to 8192    
13 8279 to 8371   Gr

CDS of F6P23.2 from the initial methionine to the termination codon

ATGGAGGATGGAATTGGAGGGCTGAGACACATGTTGGCGACGGTTTTTCTTTCTGCCTTC
GCCGGATTCATGGTAGTACCAGTGATTACTGATGTCACCGTCGCTGCTGTATGCTCTGGC
CCTGACGACTCATGTTCTCTTGCCGTTTACCTCACTGGTTTTCAACAAGTGGCAATAGGG
ATGGGGACTATGATAATGATGCCAGTAATTGGGAATCTATCAGACAGATATGGGATTAAA
ACAATCCTCACCCTTCCCATGTGTCTCTCCATTGTTCCTCCTGTAATTTTAGGATACAGA
AGGGATATAAAATTCTTTTATGTGTTTTATATAAGCAAGATTCTGACCTCTATGGCAGTA
AATATTCATGGCAGTACAAGAATTTCAGCTTTTGGTATTTTAGCTGGTATCAAAACAATT
GCTGGACTTTTTGGAACATTAGTTGCTCGTTTTCTTCCCATAGCTTTGACTTTTCAGGTT
TCTGCTATATCATTTTTCGTTGGCCTAGTGTACATGAGAGTTTTCCTAAAAGAAAAGCTA
AACGATGATGAAGATGATGATCTTCATCATGGTACGTATCATCAAGAGGATCATGATAGT
ATTAATACCACAATGTTAGCAGAGCCGATTCTGAACGATAGACCCATCAAAACACAAGTC
TTTCACAAGAAGTATTCTTCATTGAAAGATATGATCAGCTTGATGAAGACTAGGTATTAC
TTTTTGAAAGCACGTTTTGGATTTGACAAGAAGCAGTTTGCTGATCTCTTGCTGTTGATT
ACCATCGTTGGGTCTATCTCTCAATTGTTTGTACTGCCAAGATTTGCATCGGCCATTGGA
GAATGCAAGCTTTTATCAACAGGGCTTTTTATGGAATTCATAAACATGGCCATTGTCAGC
ATTTCTTGGGCACCATGGGTTCCTTATCTCACCACTGTGTTTGTACCTGGAGCCTTATTT
GTGATGCCATCGGTTTGTGGTATTGCCTCGAGACAAGTTGGACCTGGTGAACAGGGAAAA
GTTCAAGGATGCATATCTGGAGTGAGGTCCTTTGGCAAAGTGGTGGCACCATTTGTGTTC
AGTCCATTGACAGCGTTGTTTCTTTCAAAAAATGCACCGTTCTATTTCCCTGGATTCAGC
CTCCTTTGCATCTCCTTGTCCTCGTTGATCGGTTTCTTTCAGAGTCTACTGATTAAAGAT
GTCCCTACTCCACCATTGAACAAAGCAATCAACAAAACCTCAGGCGAGGAAGTGTGA

 

Protein translation:

MEDGIGGLRHMLATVFLSAFAGFMVVPVITDVTVAAVCSGPDDSCSLAVYLTGFQQVAIG
MGTMIMMPVIGNLSDRYGIKTILTLPMCLSIVPPVILGYRRDIKFFYVFYISKILTSMAV
NIHGSTRISAFGILAGIKTIAGLFGTLVARFLPIALTFQVSAISFFVGLVYMRVFLKEKL 
NDDEDDDLHHGTYHQEDHDSINTTMLAEPILNDRPIKTQVFHKKYSSLKDMISLMKTRYY
FLKARFGFDKKQFADLLLLITIVGSISQLFVLPRFASAIGECKLLSTGLFMEFINMAIVS
ISWAPWVPYLTTVFVPGALFVMPSVCGIASRQVGPGEQGKVQGCISGVRSFGKVVAPFVF
SPLTALFLSKNAPFYFPGFSLLCISLSSLIGFFQSLLIKDVPTPPLNKAINKTSGEEV*

 

An alignment of F6P23.1, F6P23.2, mouse tetracycline transporter-like protein (Mustettport) and the tetracyline resistance gene product, tetA, from E. coli is presented below.

             1                                                         60
    F6P23.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F6P23.2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MEDGIGGLRHMLATVFLSAFAGFMVVPVITD 
E.coli tetA  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
Mustettport  MTQGKKKKRAANRSIMLAKKIIIKDGGTPQGIGSPSVYHAVIVIFLEFFAWGLLTAPTLV 

             51                                                       120
    F6P23.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F6P23.2  VTVAAVCSGPDDSCSLAVYLTGFQQVAIGMGTMIMMPVIGNLSDRYGIKTILTLPMCLSI 
E.coli tetA  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~VDYAIMA 
Mustettport  VLHETFPKHTFLMNGLIQGVKGLLSFLSAPLIGALSDVWGRKSFLLLTVFFTCAPIPLMK 

             121                                                      180
    F6P23.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F6P23-2  VPP..VILGYRRDIKFFYVFYISKILTSMAVNIHGSTRISAFGILAGIKTIAGLFGTLVA 
E.coli tetA  TAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLG 
Mustettport  ISPWWYFAVIS..VSGVFAVTFSVVFAYVADITQEHERSMAYGLVSATFAASLVTSPAIG 

             181                                                      240
    F6P23.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F6P23.2  RFLPIALTFQVSAISFFVGLVYMRVFLKEKLNDDEDDDLHHGTYHQEDHDSINTTMLAEP 
E.coli tetA  GLMGG.FSPHAPFFAAAALNGLNFLTGCFLLPESHKGERR......PLRREALNPLASFR 
Mustettport  AYLGQMYGDSLMVVLATAIALLDICFILVAVPESLPEKMRPASWGAPISWEQADPFASLK 

             241                                                      300
    F6P23.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F6P23.2  ILNDRPIKTQVFHKKYSSLKDMISLMKTRYYFLKARFGFDKKQFADLLLLITIVGSISQL 
E.coli tetA  WARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQA 
Mustettport  KVGQNSIVLLICITVFLSYLPEAGQYSSFFLYLKQIMKFSPKSVAAFIAVLGILSIIAQT 

             301                                                      360
    F6P23.1  ~~~~~~~~~~~~~~~~~~~~~~EFFNATCLSVAWSPWVPYAMTMLVPGAMFVMPSVCGIA 
    F6P23.2  FVLPRFASAIGECKLLSTGLFMEFINMAIVSISWAPWVPYLTTVFVPGALFVMPSVCGIA 
E.coli tetA  MITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAML 
Mustettport  IVLSLLMRSIGNKNTILLGLGFQILQLAWYGFGSEPWIMWAAGAVAAMSSITFPAVSALV 


             361                                                      420
    F6P23.1  SRQVGSSEQGKVQGCISGVRAFAQVVAPFVYSPLTALFLSENAPFYFPGFSILCIAISLM 
    F6P23-2  SRQVGPGEQGKVQGCISGVRSFGKVVAPFVFSPLTALFLSKNAPFYFPGFSLLCISLSSL 
E.coli tetA  SRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPA 
Mustettport  SRTADADQQGVVQGMITGIRGLCNGLGPALYGFIFYIFHVELKELP.ITGTDLGTNTSPQ 

             421                                                      480
    F6P23.1  IGFLQSLLIKDHPSLPLVNITINNPSKAEA*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F6P23.2  IGFFQSLLIKDVPTPPL.NKAINKTSGEEV*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
E.coli tetA  LRRGLWSGAGQRADR*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
Mustettport  HHFEQNSIIPGPPFLFGACSVLLALLVALFIPEHTNLSLRVQQLEEALWQSQPSSQYTSA 

             481                                             531
    F6P23.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F6P23.2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
E.coli tetA  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
Mustettport  RRGQRTFTPGHECVTEIRKTSLSRSTQVLVFTCSSGCTFHFHLQCTLRLS*

 

written 4 Aug 97
Larry Parnell