Gene F6P23.14
Identification isocitrate dehydrogenase subunit 2
Position 81401 to 83126, from the 5'-UTR through the CDS to the 3'-UTR
Strand -
EST match R29962, R29955, T43648, H36047, and others
Database match A. thaliana NAD+-dependent isocitrate dehydrogenase subunit 2 (IDH2)

 

CDS:  The table below lists the coordinates of the 4 exons for F6P23.14 and which gene prediction algorithms determined the 3' and 5' termini of each exon (Fe = FEXA, Fg = FGENEA, GS = GenScan, Gr = GRAIL, M = MZEF).

Exon Range 3' 5'
1 82545 to 83054 Fe,Fg,GS,M Fe,Fg,GS,Gr
2 82172 to 82411 Fg,GS,Gr,M Fg,GS,Gr,M
3 82014 to 82092 Fe,Fg,GS,Gr,M Fe,Fg,GS,Gr,M
4 81650 to 81924 GS,Gr GS

Complete CDS of F6P23.14

ATGTCTCGCCAATCGTTTTCTCTACTGAAGAATCTTCGTAGCATCGCTTCCGGTTCCAAA
ATCCAGACCCGATCCGTGACTTACATGCCCAGACCCGGTGACGGAAAACCAAGACCAGTG
ACGTTAATCCCCGGAGACGGAGTTGGTCCGTTGGTAACAAACGCGGTTCAACAAGTGATG
GAAGCGATGCACGCTCCGGTTTACTTCGAACCGTTCGAAGTTCATGGAGATATGAAGAGT
TTACCTGAAGGATTGTTGGAATCGATTAAGAAGAACAAAGTTTGTTTGAAAGGTGGTCTT
AAGACTCCCGTCGGTGGTGGTGTGAGCTCTCTCAATGTTAATCTGAGAAAGGAGCTTGAT
CTCTTTGCTTCTTTGGTCAACTGTTTCAATTTGCCTGGTTTAGCTTCTCGTCATGAGAAT
GTTGATATTGTTGTGATTAGAGAGAACACTGAAGGTGAATATGCAGGTCTTGAACATGAA
GTTGTTCCTGGTGTTGTTGAGAGCCTTAAGGTGATTACAAAGTTCTGTTCAGAGCGTATT
GCAAAGTATGCGTTTGAGTATGCCTACTTGAACAACAGGAAGAAAGTTACAGCAGTGCAC
AAGGCTAATATCATGAAATTGGCTGATGGTTTGTTCTTGGAGTCTTGTCAAGAGGTTGCT
AAGAAGTATCCAAGCATTGCTTACAATGAAATCATTGTTGATAATTGTTGTATGCAACTT
GTGGCTAGACCAGAGCAATTCGATGTCATGGTGACGCCTAATCTCTATGGTAATCTAGTG
GCAAACACTGCTGCTGGTATTGCTGGAGGTACTGGAGTCATGCCTGGAGGAAATGTTGGG
GCGGAGTATGCGGTCTTTGAGCAAGGTGCATCAGCGGGAAACGTGGGGAAGGATACAACG
GAAGAGCAAAAGAATGCAAACCCTGTGGCTTTGTTGCTGTCATCAGCCATGATGCTTAGA
CACCTTCAGTTCCCTTCTTTTGCTGACAGGCTTGAAACCGCTGTGAAGCGTGTTATCGCT
GAAGGGAATTGTCGGACTGAAGATCTTGGTGGAAACAGTACCACTCAAGAGGTCGTTGAC
GCTGTCATTGCAAATTTGGATTGA

 

Protein translation: 

MSRQSFSLLKNLRSIASGSKIQTRSVTYMPRPGDGKPRPVTLIPGDGVGPLVTNAVQQVM
EAMHAPVYFEPFEVHGDMKSLPEGLLESIKKNKVCLKGGLKTPVGGGVSSLNVNLRKELD
LFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVVESLKVITKFCSERI
AKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCQEVAKKYPSIAYNEIIVDNCCMQL
VARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGAEYAVFEQGASAGNVGKDTT
EEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNSTTQEVVD
AVIANLD*

 

Biochemical reaction catalyzed by isocitrate dehydrogenase

     Isocitrate dehydrogenase catalyzes the oxidative decarboxylation of isocitrate to oxalosuccinate, a very labile compound which quickly loses CO2 while bound to the enzyme to yield a-ketoglutarate.

   COO-                     COO-                      COO-
   |       NAD+             |       H+                |
   CH2      \               CH2       \               CH2
   |         \              |          \              |
 H-C-COO-  ----------->  H-C-COO-  ------------->   H-C-H
   |            \           |              \          |
 H-C-OH          \          C=O             \         C=O
   |           NADH + H+    |               CO2       |
   COO-                     COO-                      COO-

Isocitrate             Oxalosuccinate            a-Ketoglutarate

 

written 19 Oct 97
Larry Parnell