Sulfate Transport Proteins

In humans, the DTD gene is thought to be involved in sulfanation of proteoglycans (Hastbacka et al., 1994). Deficiencies in the DTD protein are thought to be a leading factor in diastrophic dysplasia. Expression of the human DRA gene is significantly decreased in adenomas and adenocarcinomas of the colon (Schweinfest et al., 1993). Rat SAT1a is a canalicular sulfate transporter of rat liver. SAT1a encodes a saturable Na+-independent sulfate transporter and twelve putative transmembrane spanning domains are suggested by computer-based hydrophobicity analysis.

Sulfate is taken up by plants either through the roots from the soil or through the leaves from acidified rainfall. In spinach chloroplasts, the uptake of sulfate induces a release of phosphate which in turn lowers CO2-dependent O2 evolution (Mourioux & Douce, 1979). It was also found that sulfate inhibits glutamate synthesis in intact spinach chloroplasts and that this inhibition correlates with a decline in the stromal Pi concentration (Dumas et al., 1989). Three sulfate transporter genes have been described for Stylosanthes hamata. Smith, et al. (1995) describe ShamataST1 and ShamataST2 as high affinity H+/sulfate cotransporters expressed in roots and ShamataST3 as a lower affinity H+/sulfate cotransporter that may be involved in sulfate transport within the plant. Soybean AKI70 is a nodulin-specific gene whose transcripts first appear in roots 6-7 days after inoculation with Bradyrhizobium (Kouchi & Hata, 1993)

Alignment of Sulfate Transport Proteins

The alignment displayed below contains 24 eukaryotic sulfate transport proteins. In this alignment are six Arabidopsis sequences (listed in the alignment with the prefix Athal); AthalMSH12.1 is a partial sequence and encoded at the end of PAC clone MSH12. There are seven sequences from other plants (soybean, barley, S. hamata, and S. stapfianus). Other sequences in this alignment are derived from S. cerevisiae (Ysc), Neurospora (Ncrass), C. elegans (Ce), rat, mouse, and human. Several of the sequences are reported to encode up to 12 transmembrane domains. The four C. elegans sequences, one from S. cerevisiae and two from Arabidopsis were identified through genome sequencing initiatives.


                 1                                                                        75
        YscSUL1  ~~~~~~~~~~~~~~MSRKSSTEYVHNQEDADIEVFESEYRTYRESEAAENRDGLHNGDEENWKVNSSKQKFG..V 
        YscSEL2  MSREGYPNFEEVEIPDFQETNNTVPDLDDLELEY..DQYKNNENNDTFNDKDLESNSVAKHNAVNSSKGVKGSKI 
      NcrassSP2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MASTSTGETV 
         HumDTD  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSSESKEQHNVSPRDSAEGNDSYPSGIHLELQRESSTDFKQFE 
        MusSTOB  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSSENKEQHDLSPRDLPEEAFGFPSELPLETQRRSGTDLRQSE 
       RatSAT1a  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MDAS 
         HumDRA  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MIEPFGNQ 
     CeK12G11.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MAPPGRINHARHLSEAEEEAENYLEHFYNKSEPNKRRVSFVQRG 
     CeK12G11.2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MIHVEEEEYDQLRSFYAKK..........RRA 
      CeZK287.2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     CeF14D12.5  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
AthalSTab003591  ~~~~~~~~~~~~~~~~~~~~~~MKERDSESFESLSHQVLPNTSNSTHMIQMAMANSGSSAAAQAGQDQ.PDRSKW 
     ShamataST3  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSSLGTEQFSERSQW 
     AthalSTh14  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MGIELQNHQSHHEEASPAEEPMSRW 
       SoyAKI70  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MAHQW 
       AthalST1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MGTEDYTFPQGAEELHRRHH 
AthalSTab004060  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSSKRASQYH 
     ShamataST1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSQRVSDQVMADVIAETRSNSSSHRHGGGGGGDDTTSLPYM.H 
     ShamataST2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSQRVSDQAMAEVIAETRTNSSSRRH...GGGDDTPSLPYM.H 
      BarleyST1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MPRTVSDGG.EDFDGDVCSQTASQRH......TDSTHHHHG.Y 
   AthalT3F12.7  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSGTINPPDGGGSGARNPPVVRQ 
        SstapST  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MVGMRVPYGGSYTNNGSNESQPPGAAPEVPAM.VEVH 
   AthalMSH12.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSYASLSVKDLTSLVSRSGTGSSSSLKP 
      YscPR003c  ~~~~~~~~~~~~~~~~~~~~~~MTSNNSLLGRGRMSYSSTAPPRFKRSVDQRDTFSDNFDYDKDSSNRGRTYIAA 

                 76                                                                      150
        YscSUL1  TKN..ELSDV.LYD.SIPAYEESTVTLKEYYDHSIKNNLTAKSAGSYLVSLFPIIKWFPHYNF.TWGYADLVAGI 
        YscSEL2  DYF..NPSDVSLYDNSVSQFEETTVSLKEYYDHSIRSHLTVKGACSYLKSVFPIINWLPHYNF.SWFTADLIAGI 
      NcrassSP2  KKFLGIPADERLNDTACYVDGSFVESEPTTQDFLNEIRPTVQGTLNYLRELFPFVNWIFHYNL.TWLLGDFIAGV 
         HumDTD  TNDQCRPYHRILIERQEKSDTNFKEFVIKKLQKNCQ..CSPAKAKNMILGFLPVLQWLPKYDLKKNILGDVMSGL 
        MusSTOB  TGHGRRAFRRIHMELREKPDTDIKQFVIRELQKSCQ..CSAAKVRDGAFDFFPVLRWLPKYDLKKNILGDVMSGL 
       RatSAT1a  PEPPQKGGTLVLVRRQPPVSQGLLETLKARLKKSCT..CSMPCAQALVQGLFPVIRWLPQYRLKEYLAGDVMSGL 
         HumDRA  YIVARPVYSTNAFEENHKKTGRHHKTFLDHLKVCCS..CSPQKAKRIVLSLFPIASWLPAYRLKEWLLSDIVSGI 
     CeK12G11.1  AMNQAQFDEKFDYNKPHLE.NELKKQAKKFVRRFYEPFTSFFALKLFIFDLIPILKWFPEYKWKTDLSLDIIGGI 
     CeK12G11.2  PMNQVEYDEKYGYQKRLKDGGKFKKRSTKVASRYYVPFTSVTNFKIFLLNLFPIFGWLPKYDWKNSLTSDVVGGI 
      CeZK287.2  ~~~~~~~~~~~~~~~~~~~~MKEVYQPCTTTDQDIVEKKECSPFRKKLQKYIPILEWLPNYQWKDHFHGDVIAGL 
     CeF14D12.5  ~MKQEEYDQMYSFNREGNETWVKKK......INNYCTFLSKDGIRKIILRRVPVIDWIGSYQ.INNFASDFIAGL 
AthalSTab003591  LLDCPEPPSPWHELKRQVKGSFL..TKAKK.FKSLQKQPFPKQILSVLQAIFPIFGWCRNYK.LTMFKNDLMAGL 
     ShamataST3  VLNSPNPP....PLTKKFLGPL....KDNK.FFTSSSSKKETRAVSFLASLFPILSWIRTYS.ATKFKDDLLSGL 
     AthalSTh14  LINTPEPPSMWQELIGYIRTNVL..AKKKH.KRNKTKNSSSNLVYSCLKSAFPILSWGRQYK.LNLFRKDLMAGL 
       SoyAKI70  VLNAPEPPSMLRQVVDNVKETLLPHPNPNT.FSYLRNQPFSKRAFALLQNLFPILASLQNYN.AQKLKCDLMAGL 
       AthalST1  TVEAPQPQPFLKSLQYSVKETLFPDDPFRQ.FKN..QN.ASRKFVLGLKYFLPIFEWAPRYN.LKFFKSDLIAGI 
AthalSTab004060  QVEIPPPQPFLKSLKNTLNEILFADDPFRR.IRN..ESKTSKKIELGLRHVFPILEWARGYS.LEYLKSDVISGI 
     ShamataST1  KVGTPPKQTLFQEIKHSFNETFFPDKPFGK.FKD..QSG.FRKLELGLQYIFPILEWGRHYD.LKKFRGDFIAGL 
     ShamataST2  KVGAPPKQTLFQEIKHSFNETFFPDKPFGN.FKD..QSG.SRKFVLGLQYIFPILEWGRHYD.LKKFRGDFIAGL 
      BarleyST1  KVGFPPAKGVFAEFAEGVKETFFADDPLRE.YKD..QPR.SKKLWLSLVHLFPVLDWSRSYT.FGKFKGDLVAGL 
   AthalT3F12.7  RVLAPPKAGLLKDIKSVVEETFFHDAPLRD.FKG..QTP.AKKALLGIQAVFPIIGWAREYT.LRKFRGDLIAGL 
        SstapST  KVVPPPPQSTASKLKTRLKETLFPDDPFRG.FQG..QPARV.QWVLAVKYLFPILDWLPAYS.LSLFKSDLIAGL 
   AthalMSH12.1  PGQTRPVKVIPLQHPDTSNEARPPSIPFDDIFSGWTAKIKRMRLVDWIDTLFPCFRWIRTYRWSEYFKLDLMAGI 
      YscPR003c  SNSTTGVPPPNNSRSGCTNNTNNTNNTSNTSNTNNNDSVDENTVFETLPYYLPCFSWLPEYTF.NKLWGDVIAGI 

                 151                                                                     225
        YscSUL1  TVGCVLVPQSMSYAQ.IASLSPEYGLYSSFIGAFIYSLFATSKDVCIGPVAVMSLQTAKVI.............. 
        YscSEL2  TIGCVLVPQSMSYAQ.VATLPAQYGLYSSFIGAYSYSFFATSKDVCIGPVAVMSLQTAKVI.............. 
      NcrassSP2  TVGFVVVPQGMAYAK.LANLAPEYGLYTSFVGFVLYWAFATSKDITIGAVAVMSTIVGNII.............. 
         HumDTD  IVGILLVPQSIAYSL.LAGQEPVYGLYTSFFASIIYFLLGTSRHISVGIFGVLCLMIGETVDRELQKAGYDNAHS 
        MusSTOB  IVGILLVPQSIAYSL.LAGQEPIYGLYTSFFASIIYFLFGTSRHISVGIFGILCLMIGEVVDRELHKACPDTDAT 
       RatSAT1a  VIGIILVPQAIAYSL.LAGLQPIYSLYTSFFANLIYFLMGTSRHVNVGIFSLLCLMVGQVVDRELQLAGFD..PS 
         HumDRA  STGIVAVLQGLAFAL.LVDIPPVYGLYASFFPAIIYLFFGTSRHISVGPFPILSMMVGLAVSGAVSKAVPDRNAT 
     CeK12G11.1  TVGVMQVPQGIAYAL.LAKQPAINGLYTSLFPPLIYMLFGTSRHASLGTFAVVSLMTGLSVEKLA.......... 
     CeK12G11.2  TVGVLQIPQGIAYAI.LSRQDPIVGLYTSIYPVFLYIFFGTSKHASLGTFAVVALMTGLAVEREAF......... 
      CeZK287.2  TVGIMHVPQGMAYAS.LAGVPPVYGMYSSFFASTIYMFFGTARHISIGVFAVASMMVGAARLRLAP......... 
     CeF14D12.5  TMGVYNVPQAMSYSI.LAGLPPVYGLYASFFPPFLYSIFGSAKHSSIGVFSITCLMVDKCVKKMLK......... 
AthalSTab003591  TLASLCIPQSIGYAT.LAKLDPQYGLYTSVVPPLIYALMGTSREIAIGPVAVVSLLISSMLQKLIDPETDPLGYK 
     ShamataST3  TLASLSIPQSIGYAN.LAKLDPQYGLYTSVIPPVIYALMGSSREIAIGPVAVVSMLLSSLVPKVIDPDAHPNDYR 
     AthalSTh14  TLASLCIPQSIGYAN.LAGLDPEYGLYTSVVPPLIYSTMGTSRELAIGPVAVVSLLLSSMVRDLQDPVTDPIAYR 
       SoyAKI70  TLAIFAIPQCMGNAT.LARLSPEYGLYTGIVPPLIYAMLASSREIVIGPGSVDSLLLSSMIQTLKVPIHDSSTYI 
       AthalST1  TIASLAIPQGISYAK.LANLPPILGLYSSFVPPLVYAVLGSSRDLAVGTVAVASLLTGAMLSKEVDAEKDPKLYL 
AthalSTab004060  TIASLAIPQGISYAQ.LANLPPILGLYSSLVPPLVYAIMGSSRDLAVGTVAVASLLTAAMLGKEVNAVVNPKLYL 
     ShamataST1  TIASLCIPQDLAYAK.LANLDPWYGLYSSFVAPLVYAFMGTSRDIAIGPVAVVSLLLGTLLSNEISNTKSH.DYL 
     ShamataST2  TIASLCIPQDLAYAK.LANLDPWYGLYSSFVAPLVYAFMGTSRDIAIGPVAVVSLLLGTLLSNEISNTKSH.DYL 
      BarleyST1  TIASLCIPQDIGYAK.LANLQPHVGLYSSFVPPLIYALMGSSRDIAIGPVAVVSLLLGTLLQEEIDPVKNPLEYS 
   AthalT3F12.7  TIASLCIPQDIGYAK.LANVDPKYGLYSSFVPPLIYAGMGSSRDIAIGPVAVVSLLVGTLCQAVIDPKKNPEDYL 
        SstapST  TIASLAIPQGISYAK.LANLPPLIGLYSSFVPPLVYAVLGSSRDLAVGPVSISSLIMGPCCASR.QPHCGADAVP 
   AthalMSH12.1  TVGIMLVPQAMSYAK.LAGLPPIYGLYSSFVPVFVYAIFGSSRQLAIGPVALVSLLVSNALGGIAD..TNEELHI 
      YscPR003c  SVASFQIPLALSYTTSIAHVPPLCGLYSLAISPFVYGILGSVPQMIVGPESAISLVVGQAVE............. 

                 226                                                                     300
        YscSUL1  ................AEVLKKYPEDQTEVTAPIIATTLCLLCGIVATGLGILRLGFLVELISLNAVAGFMTGSA 
        YscSEL2  ................ADVTAKYPDGDSAITGPVIATTLALLCGIISAAVGFLRLGFLVELISLNAVAGFMTGSA 
      NcrassSP2  ................ANVQKDHPDFD....AGDIARTLAFISGAMLLFLGLIRFGFIVEFIPIVAISAFMTGSA 
         HumDTD  APSLGMVSNGSTLLNHTSDRI....CDKSCYAIMVGSTVTFIAGVYQVAMGFFQVGFVSVYLSDALLSGFVTGAS 
        MusSTOB  SSSIAVFSSGCVVVNHTLDGL....CDKSCYAIKIGSTVTFMAGVYQVAMGFFQVGFVSVYLSDALLSGFVTGAS 
       RatSAT1a  QDSLGPGNNDSTLNNTATLTVGLQDCGRDCHAIRIATALTLMAGLYQVLMGILRLGFVSTYLSQPLLDGFAMGAS 
         HumDRA  TLGLPNNSNNSSLLDDERVRVAA............AASVTVLSGIIQLAFGILRIGFVVIYLSESLISGFTTAAA 
     CeK12G11.1  ...APTDYDPSSFNE......TDIDLVKLPSPTEVSCAITITMGLILFVMGVLRLQFLTTYLSDQVIAGFTVGSS 
     CeK12G11.2  ...IPSDNLNSTLLP......GD...EALPSPIEVSCALVLGVGLIQFLMGVFRLQFLTTYLSDQLIAGFTTGSA 
      CeZK287.2  ...DIPISNSSDINP......SVYPLGEYVDPLVFTSALTLLVGVVQIIMGILRLGFLTTYLSDSLVSGFTTGAA 
     CeF14D12.5  ...FRNENPESELI.......SIFSDKLYNFWAQNSPA..........VMAIFRCDKPMKFLGAPAISAITFSAC 
AthalSTab003591  KLVL.................................TTTFFAGIFQASFGLFRLGFLVDFLSHAAIVGFMGGAA 
     ShamataST3  NLVF.................................TVTLFAGIFQTAFGVLRLGFLVDFLSHAALVGFMAGAA 
     AthalSTh14  KIVF.................................TVTFFAGAFQAIFGLFRLGFLVDFLSHAALVGFMAGAA 
       SoyAKI70  QLVF.................................TVTFFAGIFQVAFGLFRFGFLVEHLSQATIVGFLAAAA 
       AthalST1  HLAF.................................TATFFAGVLEASLGIFRLGFIVDFLSHATIVGFMGGAA 
AthalSTab004060  HLAF.................................TATFFAGLMQTCLGLLRLGFVVEILSHAAIVGFMGGAA 
     ShamataST1  RLAF.................................TATFFAGVTQMLLGVCRLGFLIDFLSHAAIVGFMAGAA 
     ShamataST2  RLAF.................................TATFFAGVTQMLLGVCRLGFLIDFLSHAAIVGFMAGAA 
      BarleyST1  RLAF.................................TATFFAGITQAMLGFFRLGFIIEFLSHAAIVGFMAGAA 
   AthalT3F12.7  RLVF.................................TATFFAGIFQAGLGFLRLGFLIDFLSHAAVVGFMGGAA 
        SstapST  AARL.................................HATLFAGIFQASLGILRLGFIIDFLSKATLVGFMAGAA 
   AthalMSH12.1  ELAI.................................LLALLVGILECIMGLLRLGWLIRFISHSVISGFTSASA 
      YscPR003c  .....................SITLHKENVSLIDISTVITFVSGTILLFSGISRFGFLGNVLSKALLRGFISSVG 

                 301                                                                     375
        YscSUL1  FNIIWGQIPALMGYNSLVNTREATYKVVINTLKHLPNTKLDAVFGLIPLVILYVWKWWCGTFGITLADRYYRNQP 
        YscSEL2  FNILWGQVPALMGYNSLVNTRAATYKVVIETLKHLPDTKLDAVFGLIPLFLLYVWKWWCGTYGPRLNDRYNSKNP 
      NcrassSP2  ISIAAGQVSTLMGIPN.INSREETYKVIINTLKGLPNTHLDA.HGLDRA....IWAVLYPLVLYQMGKRYPRQQ. 
         HumDTD  FTILTSQAKYLLGLN...LPRTNGVGSLITTWIHVFRNIHKTNLCDLITSLLCLLVLLPTKE....LNEHFKS.. 
        MusSTOB  FTILTSQAKYLLGLS...LPRSHGVGSVITTWIHIFRNIRNTNICDLITSLLCLLVLVPSKE....LNEHFKD.. 
       RatSAT1a  VTILTSQAKHLLGVR...IPRHQGLGMVIHTWLSLLQNVGQANLCDVVTSAVCLAVLLTAKE....LSDRYRH.. 
         HumDRA  VHVLVSQLKFIFQLT...VPSHTDPVSIFKVLYSVFSQIEKTNIADLVTALIVLLVVSIVKE....INQRFKD.. 
     CeK12G11.1  VHVLVSQLKTLLGIRG..LPRHSGPFYLFRHLYDLVMSITRANPVSCGISIVSIIVLHFGKEFINPFIKR.KT.. 
     CeK12G11.2  VHVLVSQFKELFGLRG..LVKHSGPGYLIRNVYDTVTNLPKANFMCCAISLATMILLHCGKEYINPIMKR.KM.. 
      CeZK287.2  VHVFTSQLNKVFGIK...LPRHEGIGMIVRMYRDMIMSLGSVNFVALGISIFGILFLDLGRTYINPIVKK..... 
     CeF14D12.5  FFGVVSQIPKLCGFSV..PSRNEHWFSLVHSILDIFENCHKSNTATLCISASALVFLIGSRIFIEPFFKNHKK.. 
AthalSTab003591  IVIGLQQLKGLLGIT..NFTTNTDIVSVLRAVWRSCQQQ......WSPHTFILGCSFLSFILITRFIGKKYKK.. 
     ShamataST3  IVIGLQQLKGLLGLT..HFTTKTDAVAVLKSVYTSLHQQITSSENWSPLNFVIGCSFLIFLLAARFIGRRNKK.. 
     AthalSTh14  IVIGLQQLKGLFGLT..HFTNKTDVVSVLSSVFHSLH......HPWQPLNFVIGSSFLIFILLARFIGKRNNK.. 
       SoyAKI70  VGIGLQQLKGLFGID..NFNNKTDLFSVVKSLWTSFKNQSA....WHPYNLIIGFSFLCFILFTRFLGKRNKK.. 
       AthalST1  TVVSLQQLKGIFGL..KHFTDSTDVISVMRSVFSQT.......HEWRWESGVLGCGFLFFLLSTRYFSIKKPK.. 
AthalSTab004060  TVVCLQQLKGLLGL..HHFTHSTDIVTVLRSIFSQS.......HMWRWESGVLGCCFLIFLLTTKYISKKRPK.. 
     ShamataST1  ITIGLQQLKGLLGISNNNFTKKTDIISVMRSVWTHVH......HGWNWETILIGLSFLIFLLITKYIAKKNKK.. 
     ShamataST2  ITIGLQQLKGLLGI..KDFTKNSDIVSVMHSVWSNVH......HGWNWETILIGLSFLIFLLITKYIAKKNKK.. 
      BarleyST1  ITIALQQLKGLLGIAK..FTKKSDIISVMESVWGNVQ......HGWNWQTILIGSSFLAFLLTTKYIAKKNKK.. 
   AthalT3F12.7  ITIALQQLKGFLGI..KTFTKKTDIVSVMHSVFKNAE......HGWNWQTIVIGASFLTFLLVTKFIGKRNRK.. 
        SstapST  IIVSLQQLKALLGIV..HFTTEMGIVPVMASVFHHTK......E.WSWQTILMGVCFLVFLLVARHVSIRWPR.. 
   AthalMSH12.1  IVIGLSQIKYFLGYS...IARSSKIVPIVESIIAGADKPLPLRNGKAKKE..LQFLRAAAPLTGIVLGTTIAK.. 
      YscPR003c  LVMIINSLISELKLDKFLVSLPQHYHTPFEKILFLIDYAPAQYHI...PTAIFSGCCLIVLFLTRLLKRKLMK.. 

                 376                                                                     450
        YscSUL1  KVANRLKSFYFYAQAMRNAVVIVVFTAISWSITRN.KSSKDRPISILGTVPSGLNEVGVMKI....PDGLLSNMS 
        YscSEL2  RLHKIIKWTYFYAQASRNGIIIIVFTCIGWAITRG.KSKSERPISILGSVPSGLKEVGVFHV....PPGLMSKLG 
      NcrassSP2  .......RAWFFVSTLRMVFIIILYILVSWLVNRHVKDPKKAHFKILGHVPSGFQHKGAPRL....DNEILSAIS 
         HumDTD  .........KLKAPIPIELVVVVAATLASHFGKLHEN....YNSSIAGHIPTGFMPPK......VPEWNLIPSVA 
        MusSTOB  .........KLKAPIPVELIVVVAATLASHFGKLNGN....YNSSIAGHIPTGFMPPK......APDWSLIPNVA 
       RatSAT1a  .........YLKVPVPTELLVIVVATIASHFGQLHTR....FGSSVAGNIPTGFVAPQ......IPDPKIMWSVA 
         HumDRA  .........KLPVPIPIEFIMTVIAAGVSYGCDFKNR....FKVAVVGDMNPGFQPPI......TPDVETFQNTV 
     CeK12G11.1  .........KSNIPIPWELVIVILSTVFVAVTGVDTE....AKVQVVNKIPVGVPNFS......LPSFYLIPQVL 
     CeK12G11.2  .........KSNIPIPWELVAVIISTIFVALIDANEL....YNVKIVNKIPTGLPELS......LPNPNLIPRVL 
      CeZK287.2  .........FSPIPPPLELILVIFGIVISMIFNLDAE....YHVKTVYEIPRGFPLPS......IPRLNFLPALL 
     CeF14D12.5  .........LQSIPFPKELITIVIATSASYFFDFEHR....FGVKTLHTVPRGFPYPD......VPRIDIWPYIF 
AthalSTab003591  .........LFWLPAIAPLIAVVVSTLMVFLTKADEHGVK.....TVRHIKGG.LNPMSIQDLDFNTPH.LGQIA 
     ShamataST3  .........FFWLPAIAPLLSVILSTLIVFLSKGDKHGVN.....IIKHVQGG.LNPSSVHKLQLNGPH.VGQAA 
     AthalSTh14  .........LFWIPAMAPLISVVLATLIVYLSNAESRGVK.....IVKHIKPG.FNQLSVNQLQFKSPH.LGQIA 
       SoyAKI70  .........LMWLSHVAPLLSVIGSSAIAYKINFNELQVKDYKVAVLGPIKGGSLNPSSLHQLTFDSQV.VGHLI 
       AthalST1  .........FFWVAAMAPLTSVILGSLLVYFTHAERHGV.....QVIGDLKKG.LNPLSGSDLIFTSPY.MSTAV 
AthalSTab004060  .........LFWISAMSPLVSVIFGTIFLYFLHDQFHGI.....QFIGELKKG.INPPSITHLVFTPPY.VMLAL 
     ShamataST1  .........LFWVSAISPMISVIVSTFFVYITRADKRGV.....SIVKHIKSG.VNPSSANEIFFHGKY.LGAGV 
     ShamataST2  .........LFWVSAISPMICVIVSTFFVYITRADKRGV.....TIVKHIKSG.VNPSSANEIFFHGKY.LGAGV 
      BarleyST1  .........LFWVSAIAPLISVVISTFCVYITRADKQGV.....AIVKNIKQG.INPPSFDLIYWSGPY.LAKGF 
   AthalT3F12.7  .........LFWVPAIAPLISVIISTFFVFIFRADKQGV.....QIVKHIDQG.INPISVHKIFFSGKY.FTEGI 
        SstapST  .........LFWVSACAPLVSVIISTLVVFLFKAQNHGI.....SIIGQLKCG.LNRPSWDKTNIDTTY.LGLTM 
   AthalMSH12.1  .........VFHPPSISLVGEIPQGLPTFSFPRSFDHAKTLLPTSALITGVAILESVGIAKALAAKNRYELDSNS 
      YscPR003c  .........YHKSAIFFPDILLVV..IVTILISMKFNLKHRYGISIIGDFSMDNFDELK.NPLTRPRRKLIPDLF 

                 451                                                                     525
        YscSUL1  SEIPASIIVLVLEHIAISKSFGRINDYKVVPDQELIAIGVTNLIGTFFHSYPATGSFSRSALKAKCNVRTPFSGV 
        YscSEL2  PNLPASIIVLLLEHIAISKSFGRINDYKVVPDQELIAIGVSNLLGTFFNAYPATGSFSRSALKAKCNVRTPLSGL 
      NcrassSP2  GDIPTTILVLLIEHIAISKSFGRVNNYIINPSQELVAIGFTNLLGPFLGALPRYRIILENGHQGQSRCSHASRWH 
         HumDTD  VDAIAISIIGFAITVSLSEMFAKKHGYTVKANQEMYAIGFCNIIPSFFHCFTTSAALAKTLVKESTGCHTQLSGV 
        MusSTOB  VDAIAISIIGFAITVSLSEMFAKKHGYTVKANQEMYAIGFCNIIPSFFHCITTSAALAKTLVKESTGCQTQLSAI 
       RatSAT1a  LDAMSLALVGSAFSISLAEMFARSHGYSVSANQELLAVGCCNVLPAFFHCFATSAALSKTLVKIATGCQTQLSSV 
         HumDRA  GDCFGIAMVAFAVAFSVASVYSLKYDYPLDGNQELIALGLGNIVCGVFRGFAGSTALSRSAVQESTGGKTQIAGL 
     CeK12G11.1  PDAISITVVSISVWLSISKMLAKRYNYELDSGQELFALSFASISSSFIPSIPNSCSLSRTLVAVGAGCTTQLSIF 
     CeK12G11.2  PDAISIAVVVVAVHLSLSKMLAKKYEYEIDAGQELYALSFTAIGGSFFPTFPTSIGLGRTMVGVESGVKTQVATF 
      CeZK287.2  SDAIPIAVVCYMFVMSMGKLFAKKHKYKTDATQELYAIGIASALSSFFPVYPVGASLSRSSVCEMSGANTQLYTI 
     CeF14D12.5  QDALSIAVVAYAVTMAMGQEFATKHRYRIDSNQELLALGFINIGSSFFSVFPTSASFSRTLVNERSGAKTQLSGI 
AthalSTab003591  KIGLIIAIVALTEAIAVGRSFAGIKGYRLDGNKEMVAIGFMNVLGSFTSCYAATGSFSRTAVNFAAGCETAMSNI 
     ShamataST3  KIGLISAIIALTEAIAVGRSFANIKGYHLDGNKEMLAMGCMNIAGSLTSCYVSTGSFSRTAVNFSAGCKTAVSNI 
     AthalSTh14  KIGLISAIIALTEAIAVGRSFATIKGYRLDGNKEMMAMGFMNIAGSLSSCYVATGSFSRTAVNFSAGCETVVSNI 
       SoyAKI70  RIGLTIAIISLTGSIAVGRSFASLKGHSIDPNREVVSLGIMNIVGSLTSCYIASGSLSRTAVNYNAGSETMVSII 
       AthalST1  KTGLITGIIALAEGIAVGRSFAMFKNYNIDGNKEMIAFGMMNIVGSFTSCYLTTGPFSRSAVNYNAGCKTAMSNI 
AthalSTab004060  KVGIITGVIALAEGIAVGRSFAMYKNYNIDGNKEMIAFGMMNILGSFSSCYLTTGPFSRSAVNYNAGCKTALSNV 
     ShamataST1  RVGVVAGLVALTEAIAIGRTFAAMKDYALDGNKEMVAMGTMNIVGSLSSCYVTTGSFSRSAVNYMAGCKTAVSNI 
     ShamataST2  RVGVVAGLVALTEAMAIGRTFAAMKDYSIDGNKEMVAMGTMNIVGSLTSCYVTTGSFSRSAVNYMAGCKTAVSNI 
      BarleyST1  RIGVVSGMVALTEAIAIGRTFAAMKDYQIDGNKEMVALGTMNIVGSMTSCYVATGSFSRSAVNYMAGCKTAVSNV 
   AthalT3F12.7  RIGGIAGMVALTEAVAIARTFAAMKDYQIDGNKEMIALGTMNVVGSMTSCYIATGSFSRSAVNFMAGVETAVSNI 
        SstapST  KTGLVTGIISLTEGIAVGRTFASLKEYQIDGNKEMMAIGLMNVVGSCTSCYVTTGAFSRSPVNHNAGCKTAMSNV 
   AthalMSH12.1  EVLCLTRSRIAIDNIPVLFTVFIESSTVFTFTGQLFGLGVANILGSLFSAYPAT~~~~~~~~~~~~~~~~~~~~~ 
      YscPR003c  SASLIVAMLGFFESTTASKSLGTTYNLTVSSNRELVALGFMNIVISLFGALPAFGGYGRSKINALSGAQSVMSGV 

                 526                                                                     600
        YscSUL1  FTGGCVLLALYCLTDAFFFIPKATLSAVIIHAVSDLLTSYKTTW..TFWKTNPLDCISFIVTVFITVFSSIENGI 
        YscSEL2  FSGSCVLLALYCLTGAFFYIPKATLSAVIIHAVSDLLASYQTTW..NFWKMNPLDFICFIVTVLITVFASIEDGI 
      NcrassSP2  LYRRPRPARSLCAHLRLLLHPQQR.SRRHDHPRRRPDHSSKGVY..KFWLTSPLEVVIFFAGVFVSIFTSIENGI 
         HumDTD  VTALVLLLVLLVIAPLFYSLQKSVLGVITIVNLRGALRKFRD..LPKMWSISRMDTVIWFVTMLSSALLSTEIGL 
        MusSTOB  VTALVLLLVLLVIAPLFYSLQKCVLGVITIVNLRGALLKFRD..LPKMWRLSRMDTVIWFVTMLSSALLSTEIGL 
       RatSAT1a  VSAAVVLLVLLVLAPLFHDLQRCVLACIIVVSLRGALRKVKD..LPQLWRLSPADALVWVATAATCVLVSIEAGL 
         HumDRA  IGAIIVLIVVLAIGFLLAPLQKSVLAALALGNLKGMLMQFAE..IGRLWRKDKYDCLIWIMTFIFTIVLGLGLGL 
     CeK12G11.1  FSSLIVFSVVIFLGTLLETLPMAALSAIICVALQGMFRKFAD..LIDLWKVSKIDFTIWVVSCVSTILLDVSTGL 
     CeK12G11.2  FSCLFVLSVSLYFGRFLETLPMCVLSAIIVIALKSMLWKLRD..LKGIWKLSKIDCCIWMVAFFATVLVDVSEGL 
      CeZK287.2  FSSFLLLTVILLLGPFLEPLPMCILACIVIVSLKSLFMQVKE..LPRLYRISKYDFAIWLVACLSTIFTDVTTGL 
     CeF14D12.5  TSACFMALVITTIGPYLASLPSCILSAIVIVVLESMLRKCTV..LPGLWRCSKHDFWIWIITAVVTLSSDIAQGV 
AthalSTab003591  VMAVTVFVALECLTRLLYYTPIAILASIILSALPGLI.NINE..AIHIWKVDKFDFLALIGAFFGVLFASVEIGL 
     ShamataST3  VMAVTVLLCLELFTRLLYYTPMAILASIILSALPGLI.DIGE..AYHIWKVDKFDFLACLGAFFGVLFVSIEIGL 
     AthalSTh14  VMAITVMISLEVLTRFLYFTPTAILASIILSALPGLI.DVSG..ALHIWKLDKLDFLVLIAAFFGVLFASVEIGL 
       SoyAKI70  VMALTVLMSLKFLTGLLYFTPKAILAAIILSAVPGLI.DLNK..AREIWKVDKMDFLACTGAFLGVLFASVEIGL 
       AthalST1  VMAIAVMFTLLFLTPLFHYTPLVVLSAIIISAMLGLI.DYQA..AIHLWKVDKFDFLVCMSAYVGVVFGSVEIGL 
AthalSTab004060  VMAVAVAVTLLFLTPLFFYTPLVVLSSIIIAAMLGLV.DYEA..AIHLWKLDKFDFFVCLSAYLGVVFGTIEIGL 
     ShamataST1  VMSIVVLLTLLVITPLFKYTPNAVLASIIIAAVVNLV.NIEA..MVLLWKIDKFDFVACMGAFFGVIFKSVEIGL 
     ShamataST2  VMAIVVLLTLLVITPLFKYTPNAVLASIIIAAVVNLV.NIEA..MVLLWKIDKFDFVACMGAFFGVIFKSVEIGL 
      BarleyST1  VMAIVVMLTLLLITPLFKYTPNAILASIIINAVVNLV.DYET..AYLIWKVDKMDFVALLGAFFGVVFASVEYGL 
   AthalT3F12.7  VMAIVVALTLEFITPLFKYTPNAILAAIIISAVLGLI.DIDA..AILIWRIDKLDFLACMGAFLGVIFISVEIGL 
        SstapST  IMALTVMVTLLFLMPLFVYTPNVVLGAIIIAAVIGLI.DIPA..VYHIWKMDKMDFLVCVCAFAGVLFISVQEGL 
   AthalMSH12.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
      YscPR003c  FMGVITLITMNLLLQFVHYIPNCVLSVITTIIGISLLEEVPGDIKFHLRCGGFSELFVFAVTFCTTIFYSIEAGI 

                 601                                                                     675
        YscSUL1  YFAMCWSCAMLLLKQAFPAGKFLGRVEVAEVLNPTVQEDIDAVI.....................SSNELPNELN 
        YscSEL2  YFAMCWSCAMLILKVAFPAGKFLGRVEVAEVTDAYVRPDSDVVSYVSENNNGISTLEDGGEDDKESSTKYVTNSS 
      NcrassSP2  YVTVAASGAVLLWRIAKSPGKFLGQTEIYTAPRELVR......................GSKDSGRSRKAC.... 
         HumDTD  LVGVCFSIFCVILRTQKPKSSLLGLVEESEVFESV..SAY...........KNLQTKPGIKIFRFVAP.LYYINK 
        MusSTOB  LVGVCFSMFCVILRTQKPKNSLLGLEEESETFESI..STY...........KNLRSKSGIKVFRFIAP.LYYINK 
       RatSAT1a  LAGVFFSLLSLAGRTQRPRAALLARIGDSTFYEDA..AEF...........EGLLPPPEVRVFRFTGP.LYYANK 
         HumDRA  AASVAFQLLTIVFRTQFPKCSTLANIGRTNIYKNK..KDY...........YDMYEPEGVKIFRCPSP.IYFANI 
     CeK12G11.1  IISVCFALFTTILREQYPKWHLLASVKGTQDFRDA..ERY...........GETVYFKGICIFRFDAP.LLFHNV 
     CeK12G11.2  LIAIFFALFTTILREQYPKWHLLANVKDTDEFSDT..QQY...........QETIFYKGICIFKFDAP.LLFHNV 
      CeZK287.2  VISLAFSLYTLVLRQQWPSFSTVLH.....DETPR..QNV...........PENVK.....IVKFAGS.LHFANV 
     CeF14D12.5  AAGIFTAIFTIAIQSQQPTIKLLGQIR.PNDFRPM..NHY............SLAKETSFRIIRFDAP.LIFANV 
AthalSTab003591  LVAVVISFAKIILISIRPGIETLGRMPGTDTFTDT..NQY...........PMTVKTPGVLIFRVKSALLCFANA 
     ShamataST3  LIALSISFAKILLQAIRPGVEVLGRIPTTEAYCDV..AQY...........PMAVTTPGILVIRISSGSLCFANA 
     AthalSTh14  LLAVGISFARIMLSSIRPSIEALGRLSKTDIFGDI..NQY...........PMANKTAGLLTLRISSPLLCFANA 
       SoyAKI70  AIGCRSRYHLRR*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
       AthalST1  VVAVAISIARLLLFVSRPKTAVKGNIPNSMIYRNT..EQY...........PSSRTVPGILILEIDAP.IYFANA 
AthalSTab004060  ILSVGISVMRLVLFVGRPKIYVMGNIQNSEIYRNI..EHY...........PQAITRSSLLILHIDGP.IYFANS 
     ShamataST1  LIAVAISFAKILLQVTRPRTAVLGKLPGTSVYRNI..QQY...........PKAAQIPGMLIIRVDSA.IYFSNS 
     ShamataST2  LIAVAISFAKILLQVTRPRTAVLGKLPGTSVYRNI..QQY...........PKAEQIPGMLIIRVDSA.IYFSNS 
      BarleyST1  LIAVAISLGKILLQVTRPRTALLGNLPRTTIYRNV..EQY...........PEATKVPGVMIVRVDSA.IYFTNS 
   AthalT3F12.7  LIAVVISFAKILLQVTRPRTTVLGKLPNSNVYRNT..LQY...........PDAAQIPGILIIRVDSA.IYFSNS 
        SstapST  AIAVGISVFRVLLQITRPKITVQGNIMGTDIYRNL..HQY...........KDAQRIPGFLILATEAP.INFANS 
   AthalMSH12.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
      YscPR003c  CIGCVYSIINIIKHSAKSRIQILARVAGTSNFTNL..DDYMMNMKRNSLDVEGTEEIEGCMIVRIPEP.LTFTNS 

                 676                                                                     750
        YscSUL1  KQVKSTVEV.........LPAPEYKFSVKWVPFDHGYSRELNINTTVRPPPPGVIVYRLGDSFTYVNCSRHYDII 
        YscSEL2  KKIETNVQTKGFDSPSSSISQPRIKYHTKWIPFDHKYTRELNPDVQILPPPDGVLVYRLSESYTYLNCSRHYNII 
      NcrassSP2  ..CRSRSTTAFLSLDRDDLSNPELQISTPW.....................PGIFVYRFGEGLNYVNSAKHLDNL 
         HumDTD  ECFKSAL..YKQTV.NPILIKVAWKKAAK..RKIKEKV...VTLGG....IQD..EMSVQLSHDPLEL....... 
        MusSTOB  ECFKSAL..YKKAL.NPVLVKAAWKKAAK..RKLKEEM...VTFRG....DPD..EVSMQLSHDPLEV....... 
       RatSAT1a  DFFLRSL..YSLTG.....LDAGYSATRK..DRGTEVG...VSNRS....LVDRKDLGSVSSGDGLVV....... 
         HumDRA  GFFRRKL..IDAVGFSPLRILRKRNKALRKIRKLQKQGLLQVTPKGFICTVDTIKDSDEELDNNQIEVLDQPINT 
     CeK12G11.1  ECFKKSI.................EKAYTEWQKSHEFYVLREERETILNTKLDGSDES................. 
     CeK12G11.2  ECFKKCI.................EKVYDEWKKSSEFNFVKEPNAG.KGSKFTFEGMH................. 
      CeZK287.2  TAFQDDM.................GEA................................................ 
     CeF14D12.5  EKFLDNV.................RDSTSATTKKRSASCDSQE................................ 
AthalSTab003591  SSIEERI..MGWVDEEEEEENTKSNAKRK.ILFVVLDMSSLINVDTSGITALLELHNKLIKTGVELVIVNPKWQV 
     ShamataST3  GFVRERI..LKWV.EDEEQDNIEEAAKGR.VQAIIIDMTDLTNVDTSGILALEELHKKLLSRGVELAMVNPRWEV 
     AthalSTh14  NFIRDRI..LNSVQEIEGEENEQEVLKENGLQVVILDMSCVMGVDTSGVFALEELHQELASNDIRLVIASPRWRV 
       SoyAKI70  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
       AthalST1  SYLRERI..IRWIDEEEERVKQSGESS...LQYIILDMSAVGNIDTSGISMMVEIKKVIDRRALKLVLSNPKGEV 
AthalSTab004060  TYLRDRI..GRWIDEEEDKLRTSGDIS...LQYIVLDMSAVGNIDTSGISMLEELNKILGRRELKLVIANPGAEV 
     ShamataST1  NYIKERI..LRWLIDEGAQRTESELPE...IQHLITEMSPVPDIDTSGIHAFEELYKTLQKREVQLILANPGPVV 
     ShamataST2  NYIKERI..LRWLIDEGAQRTESELPE...IQHLIVEMSPVTDIDTSGIHAFEELYKTLQKREVQLMLANPGPVV 
      BarleyST1  NYVKERI..LRWLRDEEEQQQEQKLSK...TEFLIVELSPVTDIDTSGIHALEELLKALEKRKIQLILANPGPAV 
   AthalT3F12.7  NYVRERA..SRWVREEQENAKEYGMPA...IRFVIIEMSPVTDIDTSGIHSIEELLKSLEKQEIQLILANPGPVV 
        SstapST  NYLNERI..KRWIEEESSAQTKQ..TE...LRFVILDLSAVPAIDTSGVAFLIDIKKSIEKRGLELVLVNPTGEG 
   AthalMSH12.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
      YscPR003c  EDLKQRLDRIERYGSSKIHPGRKSLRSKDSIKYVIFDLGGMTSIDSSAAQVLEEIITSYKRRNVFIYLVNVSIND 

                 751                                                                     825
        YscSUL1  FDRIKEETRRGQLITLRKKSDRPWNDPGEWKMPDSLKSLFKFKRHSATTNSDLPISNGSSNGETYEKPLLKVVCL 
        YscSEL2  TEEVKKVTRRGQLIRHRKKSDRPWNDPGPWEAPAFLKNLKFWKKRENDPESMENAPSTSVDVERDDRPLLKILCL 
      NcrassSP2  TIHVFKHTRRTELNKFEKLGDRPWNDPGP..............RRPSLPNRR...ARFAPDPAAHHPRLLR.... 
         HumDTD  ........................HTIVIDCSAIQFLDTAGIHTLKEVRRDYEAIGIQVLLAQCNPTVRDSLTNG 
        MusSTOB  ........................HTIVIDCSAIQFLDTAGIHTLKEVRRDYEAVGIQVLLAQCNPSVRDSLARG 
       RatSAT1a  ..................PLAFGFHTVVIDCAPLLFLDVAGMATLKDLRKNYRALDITLLLACCSPSVRDTLRKG 
         HumDRA  TDLPFHIDWNDDLPLNIEVPKISLHSLILDFSAVSFLDVSSVRGLKSILQEFIRIKVDVYIVGTDDDFIEKLNRY 
     CeK12G11.1  ..IDGKMFQTAQSTLNTHSPDILSRHFVIDCSGFTFIDLMGVSALKEIFSDMRKRGILVYFANAKAPVREMFEKC 
     CeK12G11.2  ..RIAPITEIPLHPGINRDP.ILPRHFVIDCSGFTFIDLMGVSALKEVFSDLRKKRVQVYFASTKVPVREMFEKC 
      CeZK287.2  ...IGKLPE........EDPLIDERTIILDASSVAFIDIMGVDALRDVFKDALKLGVHVFYCGLPEDVLSVLSND 
     CeF14D12.5  ....................DIEWSAIILDCHTWIYTDSMGIAAVKQINEELRQIRILLLFANLKSSLRRQYGNA 
AthalSTab003591  IHKLNQAKFVDRI..G.GKVYLTIGEALDACFGLKV*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     ShamataST3  IHKLKVANFVDKI..GKERVFLTVAEAVDACLSSRFANSA*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     AthalSTh14  LHKLKRAKLDEKI..KTENIYMTVGEAVDIYVRARSTSHELC*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
       SoyAKI70  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
       AthalST1  VKKLTRSKFIGDHLGKE.WMFLTVGEAVEACSYMLHTFKTEPASKNEPWNNV*~~~~~~~~~~~~~~~~~~~~~~ 
AthalSTab004060  MKKLSKSTFI.ESIGKE.RIYLTVAEAVAACDFMLHTAK..PDSPVPEFNNV*~~~~~~~~~~~~~~~~~~~~~~ 
     ShamataST1  IEKLHASKLT.ELIGED.KIFLTVADAVATYGPKTAAF*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     ShamataST2  IEKLHASNLA.ELIGED.KIFLTVADAVATYGPKTAAF*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
      BarleyST1  IQKLRSAKFT.DLIGDD.KIFLSVGDAVKKFAPKSSLNV*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
   AthalT3F12.7  IEKLYASKFV.EEIGEK.NIFLTVGDAVAVCSTEVAEQQT*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
        SstapST  HGKNTASERGTQAFQVGIACILTTGEAVASLSALAKMASP*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
   AthalMSH12.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
      YscPR003c  KVRRRLFKAGVAASVERAQANNNENNTSNTFSDAGETYSPYFDSIDAALYEIEKMKIKGNNVPNNDSESFMSNTL 

                 826                                                                     900
        YscSUL1  DFSQVAQVDSTAVQSLVDLRKAVNRYA.DRQVEFHFAGIISPWIKRSLLSVKFGTTNEEYSDDSIIAGHSSFHVA 
        YscSEL2  DFSQVAQTDATALQSLVDLRKAINQYA.DRQVEFHFVGIISPWVKRGLISRGFGTLNEEYSDESIVAGHTSYHVA 
      NcrassSP2  ..RQLHRCDRRP.RRLQDLRNQFDRYAHPDKVEWHFAGVSNRWTKRALVASGFGV..DSLRTAKVQRENHKGGVQ 
         HumDTD  EYCKKEE...ENLLFYSVYEAMAFAEVSKNQKGVCVPNGLSLSSD*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
        MusSTOB  EYCKKEE...ETLLFYSLSEAVAFAEDSQNQKGVCVVNGLSLSGD*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
       RatSAT1a  GFLGEDQGTAEELLFPSVHSAVETA.CARREELMAADSAL*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
         HumDRA  EFFDGE..VKSSIFFLTIHDAVLHILMKKDYSTSKFNPSQEKDGKIDFTINTNGGLRNRVYEVPVETKF*~~~~~ 
     CeK12G11.1  .HF.FN.FVSKENFYPTMRDATSIAR..QRQLELGFKDTGYVPEHDRLTEVLSSHPM*~~~~~~~~~~~~~~~~~ 
     CeK12G11.2  .SF.FD.FVSKENFYPTLRDATGIAR..LRQTE*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
      CeZK287.2  ENF.LS.VVPPSTFFPSI.DSCLISF..VHQQSV*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     CeF14D12.5  GLI.GEGNIGRNQMYPSIQDALDSAHELIKNHKMFFDCEKSMPPEKIVP*~~~~~~~~~~~~~~~~~~~~~~~~~ 
AthalSTab003591  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     ShamataST3  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     AthalSTh14  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
       SoyAKI70  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
       AthalST1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
AthalSTab004060  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     ShamataST1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     ShamataST2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
      BarleyST1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
   AthalT3F12.7  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
        SstapST  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
   AthalMSH12.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
      YscPR003c  FNSSLV*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 

                 901                                                                     975
        YscSUL1  KVLKDDVDYTDEDSRISTSYSNYETLCAATGTNLPFFHIDIPDFSKWDV*~~~~~~~~~~~~~~~~~~~~~~~~~ 
        YscSEL2  RVPQGEEN........PEKYSVY....TASGTNLPFFHIDIPDFAKWDI*~~~~~~~~~~~~~~~~~~~~~~~~~ 
      NcrassSP2  EVDQGPLVAIGPSVSASDIEAVVRSGSGSTDEKRPEGEGGATNGGMEKGSANGEDISTVPTATSADACACGKGRW 
         HumDTD  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
        MusSTOB  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
       RatSAT1a  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
         HumDRA  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     CeK12G11.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     CeK12G11.2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
      CeZK287.2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     CeF14D12.5  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
AthalSTab003591  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     ShamataST3  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     AthalSTh14  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
       SoyAKI70  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
       AthalST1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
AthalSTab004060  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     ShamataST1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
     ShamataST2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
      BarleyST1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
   AthalT3F12.7  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
        SstapST  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
   AthalMSH12.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
      YscPR003c  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 

                 976     986
        YscSUL1  ~~~~~~~~~~~
        YscSEL2  ~~~~~~~~~~~
      NcrassSP2  REEVGAGVWD*
         HumDTD  ~~~~~~~~~~~
        MusSTOB  ~~~~~~~~~~~
       RatSAT1a  ~~~~~~~~~~~
         HumDRA  ~~~~~~~~~~~
     CeK12G11.1  ~~~~~~~~~~~
     CeK12G11.2  ~~~~~~~~~~~
      CeZK287.2  ~~~~~~~~~~~
     CeF14D12.5  ~~~~~~~~~~~
AthalSTab003591  ~~~~~~~~~~~
     ShamataST3  ~~~~~~~~~~~
     AthalSTh14  ~~~~~~~~~~~
       SoyAKI70  ~~~~~~~~~~~
       AthalST1  ~~~~~~~~~~~
AthalSTab004060  ~~~~~~~~~~~
     ShamataST1  ~~~~~~~~~~~
     ShamataST2  ~~~~~~~~~~~
      BarleyST1  ~~~~~~~~~~~
   AthalT3F12.7  ~~~~~~~~~~~
        SstapST  ~~~~~~~~~~~
   AthalMSH12.1  ~~~~~~~~~~~
      YscPR003c  ~~~~~~~~~~~

 


created 22 Oct 97
Larry Parnell