Gene T10M13.1
Putative Identification zinc-finger protein
Position 1224 to 2168, from the initial methionine to the termination codon
Strand +
EST hits none
Database match A. thaliana proteins T13L16.6 and T32G6.11

 

CDS:  The table below lists the coordinates of the single exon comprising T10M13.1 and which exon predicting algorithms selected the exon termini (GS = GenScan, Gr = GRAIL).

Exon Range 5' 3'
1 1224 to 2168 Gr GS, Gr

GenScan predicts an initial methionine at position 1614 to 1616. This is highlighted in the CDS below.

Complete CDS of T10M13.1

ATGACATCTCTAACGATTGATCTCCTGTCAGTACTACGAAGGGAACCTTGGTTGTATAAC
AACTGGTTTGTGACAACTCATAGATGGGAGGTAAATCTTACGTTTCACCTGTTAACATCT
ATTGAATTATGGGTTCAGATGCGTGGAATTCCTCTTTTGTATGTGTGTGAGGAAACTGCT
TTAGAGATAGCACATGAGTTAGGAAAGATTCTCACTCTGGATTTTCATGACTCCACCACG
ACTCAAATAGCTTATATCCGTGTGAGAATCCGGTTTGGAATTACAGATCGCCTGAGGTTT
TTCCAAAGAATCATTTTTGATTTTGGTGAGGCTGCTCTGATAAGCTTTCAGTATGAAAGA
CTTAGGAGAATCTGTAGTAGCTGCTTCAGAATGACTCATCACAGGAACTCATGTCCTTAC
CGTCAGATTGAGCCTCTCCATAGGGTGACTAACTCCACTGCTCAAAGGAATGTTAGAGAA
GAGGTGTTCATGAGAGATGAAAATCTGAGATCTTCAATGAATTCACAATCTCAGATGTCT
GAGAGTTCGTTTCCTACTCCTATCGATCCACCTCCAAGGATTCCTCATCCTCCTTTAAAT
CCTGATGAATTGGTGGCTGCATATTACCCTCATACAAGAGCTACTTCTTTACCAAACTTT
GCAGGTCCTTTGCCTCAGGTACCTCTTAGAAAAAATGTGGATGAAAGGGATTCTAACATC
CAGCCTTTCTCCGGACCTGCATTTGCAGCACACTCTCCACGCCTAGTGGAAGTAGGTGAA
TCATCAAGGCAAGGTGAGAATACTCAGAATGTTCACACGGTGGAAAAGGGGGATTCATCA
AAAAGAAAGAACATGGGTGGTCCCAGGTTTAAAGATGATGCAAGAAAGTCCAATGAAGAT
GAGCATATGAATGGAGGGATTCTGAAACCTCCAAAAAAGCGTTGA

 

Protein sequence

MTSLTIDLLSVLRREPWLYNNWFVTTHRWEVNLTFHLLTSIELWVQMRGIPLLYVCEETA
LEIAHELGKILTLDFHDSTTTQIAYIRVRIRFGITDRLRFFQRIIFDFGEAALISFQYER
LRRICSSCFRMTHHRNSCPYRQIEPLHRVTNSTAQRNVREEVFMRDENLRSSMNSQSQMS
ESSFPTPIDPPPRIPHPPLNPDELVAAYYPHTRATSLPNFAGPLPQVPLRKNVDERDSNI
QPFSGPAFAAHSPRLVEVGESSRQGENTQNVHTVEKGDSSKRKNMGGPRFKDDARKSNED
EHMNGGILKPPKKR*

 

Alignment of T10M13.1, T13L16.6 and T32G6.11. These proteins are similar to the zinc-finger protein encoded by the Ta11 transposon. See the article by Wright DA, et al. (1996, Genetics 142:569-578) for information on this class of non-LTR retrotransposons.

           1                                                         60
 T32G6.11  MSDELWNEIQNLELGQEDPALFIPHEAYVMVEATNRLSMIARPLNPRVQNLNSVVVALPR 
 T13L16.6  MADELWDEIQNLELRQEGPSLFIPNEAYIMVAGRNRLSIIARPLNPRVQNLQAIITALPR 
 T10M13.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
Tal1-Znfp  MSSAMDRALMEMSLEEKDEPFEMPDLPEFLSCERNKLSLIGRVLNPSCQPMKHLIRNIPR 

           61                                                       120
 T32G6.11  TWGLTNQVHGRILDATYVQFLFQNEIDLMMVQRKEPWLFNNWFVAATRWEVAPAHNFVTT 
 T13L16.6  AWGLTAHVHGRIIDDTYVQFLFQSEMDLLSVQRREPWLFNNWFVASQRWQPAPALNFVTT 
 T10M13.1  ~~~~~~~~~~~~~~~~~~~~MTSLTIDLLSVLRREPWLYNNWFVTTHRWEVNLTFHLLTS 
Tal1-Znfp  KWQKEGRVTGISLTSERFQFIFDSKHDLEDVLPKGVHTYNDWSIVVDRWYENPPDDYLRF 

           121                                                      180
 T32G6.11  IDLWVQIRGIPLPYVSEETVMEIAQDLGEVLMLDYHDTTSIQIAYIRVRVRFGITDRLRF 
 T13L16.6  IDLWVQMRGIPFLYVSEETALEIAQEIGAIISLDFHDTTSTQIAYIRVRVRVGITDSLRF 
 T10M13.1  IELWVQMRGIPLLYVCEETALEIAHELGKILTLDFHDSTTTQIAYIRVRIRFGITDRLRF 
Tal1-Znfp  MLVWVQIWNIPVNYYTEKAITKLGDLIGEVKEVVFNPVEPQLHEFVRVNVLFDVSRPLRR 

           181                                                      240
 T32G6.11  FQRIVFDSGETATIRFQYERLRRICSSCFRFTHNRAYCPYR..........PRPLSIARE 
 T13L16.6  FQRITFESGESALIRFQYERLRRICSNCFRFTHNRNYCPYR..........PRVQILDRE 
 T10M13.1  FQRIIFDFGEAALISFQYERLRRICSSCFRMTHHRNSCPYRQIEPLHRVTNSTAQRNVRE 
Tal1-Znfp  SKVINFKNGDTATVRFHYERIQKRCYECQRLTHEKDLCPI.LIKERQDKASARRQGISIP 

           241                                                      300
 T32G6.11  RALFRDSVHRSSMNSQSQMTDSSFPI.PQTPPPRISHPPLNHDEFVAA.YPHLDSGRNDH 
 T13L16.6  RAALHDSVLRSSNNSQSQMTESSFPA.PLTPPPRVPAPPLNHQERV...YETGESSRRVE 
 T10M13.1  EVFMRDENLRSSMNSQSQMSESSFPT.PIDPPPRIPHPPLNPDELVAAYYPHTRATSLPN 
Tal1-Znfp  KAP.PPSVLQESDPLFGVLKESQVGVDPNTGRPRIAPEVLEGMRQYLRVANDEERSIRVD 

           301                                                      360
 T32G6.11  IRCEGESSNFRQDLSSASNSITPRREPQYLTDR.RHFEPGQSSRRHDVRDLRRGSERIGN 
 T13L16.6  DR.ESNNNLHLGDSSKRKNMIILQCEKDSQENK.QQ.ETDLNMGGIPKPPKKR*~~~~~~ 
 T10M13.1  FAGPLPQVPLRKNVDERDSNIQPFSGPAFAAHSPRLVEVGESSRQGENTQNVHTVEKGDS 
Tal1-Znfp  QVIKSVRDA.EKDPFTQKTVLQLEALPIVHHDMLREKGVVFDYDRGDSSSHNGGLNLNSS 

           361                                                      420
 T32G6.11  LNQQNYVQRSGGILKPPKKR*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
 T13L16.6  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
 T10M13.1  SKRKNMGGPRFKDDARKSNEDEHMNGGILKPPKKR*~~~~~~~~~~~~~~~~~~~~~~~~ 
Tal1-Znfp  LRLEESTQSKDARDWLTEPVNSLASKDVGCFLALSQPFPVSSTVYSPGLFESDSTGTIRK 

           421                                                      480
 T32G6.11  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
 T13L16.6  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
 T10M13.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
Tal1-Znfp  KVKQRNRPPNHARKLKLKDPSSIFVDHSLKEGSTSGTKDKRKAVDMGPSAAKIFKLNPKL 

           481     491
 T32G6.11  ~~~~~~~~~~~
 T13L16.6  ~~~~~~~~~~~
 T10M13.1  ~~~~~~~~~~~
Tal1-Znfp  VVPNEGPSKA*

 

direct repeat flanking the zinc-finger protein, potential Tn insertion site 
    1226 GACATCTCTAACGAT 1240
         |||||||||||||||
    2499 GACATCTCTAACGAT 2513

 

written 29 Jul 97
updated 5 Aug 98
Larry Parnell