Gene T10M13.14
Putative Identification glycosyl transferase
Position 72029 to 73069, from the initial methionine to the termination codon
Strand -
EST match none
Database match bacterial glycosyl transferases: lgtC of Neisseria sp., GSP-A of B. subtilis, and RfaJ of E. coli

 

CDS:  The table below lists the coordinates of the single T10M13.14 exon and which algorithms selected this exon (GS = GenScan, Gr = GRAIL).

Exon Range 3' 5'
1 72029 - 73069 Gr Gr

Complete CDS of T10M13.14

ATGCTTTGGATAACGAGATTTGCTGGATTATTCTCCGCCGCGATGGCAGTGATCGTGTTA
TCTCCGTCGCTTCAGTCATTTCCTCCGGCGGCGGCAATCCGTTCTTCTCCATCACCGATC
TTCAGAAAAGCTCCAGCGGTGTTCAACAACGGCGACGAATGTCTCTCCTCCGGCGGCGTC
TGCAATCCGTCGTTGGTCCACGTGGCGATCACGTTAGACGTAGAGTACCTGCGTGGCTCA
ATCGCAGCCGTTAACTCGATCCTTCAGCACTCGGTGTGTCCAGAGAGCGTCTTCTTCCAC
TTCATCGCCGTCTCCGAGGAAACAAACCTGTTGGAGTCGCTGGTGAGATCGGTTTTCCCG
AGACTGAAATTCAATATTTACGATTTTGCCCCTGAGACAGTTCGTGGTTTGATTTCTTCT
TCCGTGAGACAAGCTCTCGAGCAGCCTCTGAACTACGCTAGAAGCTACTTAGCGGATCTG
CTGGAGCCTTGTGTTAACCGTGTCATATACTTGGATTCGGATCTTGTCGTCGTCGATGAC
ATCGCTAAGCTTTGGAAAACTAGCCTAGGCTCGAGGATAATCGGAGCTCCGGAGTATTGT
CACGCGAATTTCACGAAATACTTCACCGGAGGATTCTGGTCGGAGGAGAGATTCTCCGGT
ACCTTTAGAGGGAGGAAGCCATGTTACTTCAACACAGGTGTGATGGTGATAGATCTTAAG
AAATGGAGAAGAGGTGGTTACACGAAACGTATCGAGAAATGGATGGAGATTCAGAGAAGA
GAGAGGATTTACGAACTAGGCTCGCTTCCACCGTTTCTTCTAGTTTTCTCCGGTCACGTG
GCTCCCATCTCTCACCGGTGGAACCAGCATGGACTTGGTGGTGACAATGTTAGAGGTAGC
TGTCGTGATTTGCATCCTGGTCCTGTGAGTTTGCTGCATTGGTCTGGTAGTGGCAAGCCC
TGGATAAGACTCGATTCCAAACGGCCTTGTCCCTTAGACGCATTATGGACGCCTTACGAC
TTGTATCGACATTCGCATTGA

 

Protein sequence

MLWITRFAGLFSAAMAVIVLSPSLQSFPPAAAIRSSPSPIFRKAPAVFNNGDECLSSGGV
CNPSLVHVAITLDVEYLRGSIAAVNSILQHSVCPESVFFHFIAVSEETNLLESLVRSVFP
RLKFNIYDFAPETVRGLISSSVRQALEQPLNYARSYLADLLEPCVNRVIYLDSDLVVVDD
IAKLWKTSLGSRIIGAPEYCHANFTKYFTGGFWSEERFSGTFRGRKPCYFNTGVMVIDLK
KWRRGGYTKRIEKWMEIQRRERIYELGSLPPFLLVFSGHVAPISHRWNQHGLGGDNVRGS
CRDLHPGPVSLLHWSGSGKPWIRLDSKRPCPLDALWTPYDLYRHSH*

 

Alignment of selected glycosyl transferases from Neisseria gonorrheae lgtC (U14554), Neisseria meningitidis lgtC (U65788), Bacillus subtilis General Stress Protein A (P25148), and E. coli RfaJ (M80599). Because this alignment indicates significant differences between the bacterial glycosyl transferases and the peptide encoded by T10M13.14, the A. thaliana sequence is considered to have an uncharacterized specificity.

           1                                                         60
 NgonlgtC  ............................................................
 NmenlgtC  ............................................................
BsubGSP-A  ...........................................................M
  EcoRfaJ  ......................................MDSFPAIEIDKVKAWDFRLANI
 A. thal.  MLWITRFAGLFSAAMAVIVLSPSLQSFPPAAAIRSSPSPIFRKAPAVFNNGDECLSSGGV

           61                                                       120
 NgonlgtC  .....MDIVFAADDNYAAYLCVAAKS.VEAAHPDTEIRFHVLDAGISEENRAAVAANLR.
 NmenlgtC  .....MDIVFAADDNYAAYLCVAAKS.VEAAHPDTEIRFHVLDAGISEANRAAVAANLRG
BsubGSP-A  RKDEIMHIVSCADDNYARHLGGMFVSLLTNMDQEREVKLYVIDGGIKPDNKKRLEETTLK
  EcoRfaJ  NTSECLNVAYGVDANYLDGVGVSITSIVLN.NRHINLDFYIIADVYNDGFFQKIAKLAEQ
 A. thal.  CNPSLVHVAITLDVEYLRGSIAAVNSILQHSVCPESVFFHFIAVSEETNLLESLVRSVFP

           121                                                      180
 NgonlgtC  GGGNIRFIDVNPEDFAGFPLNIRHISIT...TYARLKLGEYIAD..CDKVLYLDTDVLVR
 NmenlgtC  GGGNIRFIDVNPEDFAGFPLNIRHISIT...TYARLKLGEYIAD..CDKVLYLDIDVLVR
BsubGSP-A  FGVPIEFLEVDTNMY.EHAVESSHITKA...AYYRISIPDLIKDESIKRMIYIDCDALVL
  EcoRfaJ  NQLRITLYRINTDKLQCLP.CTQVWSRA...MYFRL.FAFQLLGLTLDRLLYLDADVVCK
 A. thal.  .RLKFNIYDFAPETVRGLISSSVRQALEQPLNYARSYLADLL.EPCVNRVIYLDSDLVVV

           181                                                      240
 NgonlgtC  DGLKPLWDTDLGGNWVGACIDLFVE..........RQEGYKQKIGMADGEYYFNAGVLLI
 NmenlgtC  DSLTPLWDTDLGDNWLGACIDLFVE..........RQEGYKQKIGMADGEYYFNAGVLLI
BsubGSP-A  EDISKLWDLDIAPYTVAAVED..AG..........QHERLKE.MNVTDTGKYFNSGIMII
  EcoRfaJ  GDISQLLHLGLNGAVAAVVKDVEPM..........QEKAVSRLSDPELLGQYFNSGVVYL
 A. thal.  DDIAKLWKTSLGSRIIGAPEYCHANFTKYFTGGFWSEERFSGTF.RGRKPCYFNTGVMVI

           241                                                      300
 NgonlgtC  NLKKWRRHDIFKMSCEWVEQY..KDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFM
 NmenlgtC  NLKKWRRHDIFKMSCEWVEQY..KDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFM
BsubGSP-A  DFESWRKQNITEKVINFINEHPDEDFLVLHDQDALNAILYDQWYELHPRWN....AQTYI
  EcoRfaJ  DLKKWADAKLTEKALSIL..MSKDNVYKYPDQDVMNVLLKGMTLFLPREYNTIYT....I
 A. thal.  DLKKWRRGGYTKRIEKWMEIQRRERIYELGSLPPFLLVFSGHVAPISHRWN.........

           301                                                      360
 NgonlgtC  ANGFASRHTDPLYLDRTNTAMPVAVSHYCGSAKPWHRDCT.VWGAERFTELA.GSLTTVP
 NmenlgtC  ANWFASRHTDPLYRDRTNTVMPVAVSHYCGSAKPWHRDCT.AWGAERFTELG.GSLTTVP
BsubGSP-A  MLKLKTPSTLLGRKQYNETRENPAIVHFCGGEKPWNSNTKHPYRDEYFHYMSYTKWNTI.
  EcoRfaJ  KSELKDKTHQNYKKLITESTL...LIHYTGATKPWHK..WAIYPSVKYYKIALENSPWKD
 A. thal.  QHGLGGDNVRGSCRDLHPG..PVSLLHWSGSGKPWIR.......................

           361                                       405
 NgonlgtC  EEWRGKLAVP....PTKCMLQRWRKK.........LSARFLRKIY
 NmenlgtC  EEWRGKLAVPHRMFSTKRMLQRWRRK.........LSARFLRKIY
BsubGSP-A  ....GNPAINQ..................................
  EcoRfaJ  DSPRDAKSIIEFKKRYKHLLVQHHYISGIIAGVCYLCRKYYRK..
 A. thal.  ..LDSKRPCPLDALWTPYDLYRHSH....................

 

written 30 Jul 97
updated 3 Aug 98
Larry Parnell