T10M13.17.1 and T10M13.18

This page contains the annotation of genes T10M13.17.1 and T10M13.18

Gene	T10M13.17.1
Putative Identification	hypothetical protein
Position	86229 to 89782, from the initial methionine to the termination codon
Strand	+
EST match	none
Database match	several Arabidopsis hypothetical proteins

CDS: The table below lists the coordinates of the T10M13.17.1 exons and which algorithms selected the given termini (GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene - selects splice sites only, not exons).

Exon	Range	5'	3'
1	86229 - 86321	GS, Gr	GS, Gr, NPG
2	86906 - 89782	GS, Gr, M, NPG	GS, Gr

Alternate exons not used in building the gene model: GRAIL does not select exon 1 as an initial exon. Rather, a splice site is predicted that coincides with the start methionine predicted by GenScan and this joins T10M13.17 and T10M13.17.1. Other GRAIL exons extend from 86906 to 87358, from 87788 to 88327, from 88805 to 88915, from 89145 to 89349 and from 89598 to 89782. MZEF predicts internal exons from 86906 to 87433, from 89188 to 89349 and from 90227 to 90297. NetPlantGene predicts several putative splice sites in this region. Notable sites include splice donors at 87433 (confidence score = 0.86), at 88391 (0.93) and at 90297 (0.96).

Complete CDS of T10M13.17.1

ATGGGTCTTATCAACCAATGGTTTCCACTGGACACTCTTGCAACTCCAGAGCGTATCAGG
ATGGCTTGTTTCGACGAGTTTACTTCTCCTTTTTATATGACCATGGATTCTGAGGGAGTG
TTAATGCCGTTGGTCCATGAACATCTTATGATGCCTTGGAATGATCTAAGGAAAGGTGAT
TGTTGTGGACTCTCGAAAGCTGTCAGTGTTGGTTATTATTGCAAAAGCTGCGATTTTTTC
GCCCACAAGAAATGTGGCGAGTCCTCCGAGTTTATCCAACATCCATCTCACCCCAATCAC
ACTCTTCAGCTTCGAAGTAGCGAAGGCTGTAATAGCTGCAATTTATGTGGAAGGACCATT
AGTGACCTATTCTATCGTTGTGATCTCTGTGATTTCGACGTGGATCTATATTGTGCCAAG
TATCCACCACCAGAGGTTATTGACATTCCTGAGACGCATCACCACAAGCTCAACCTTCAC
AAGGAGTTGATCGAGTTCAACTGTGATGCTAAATGTGGGAAGATTACTGGTGCTGAGTTT
CCTTACGTATGTTATGAATGTCAATTACCTTTCCATGTGGATTGCGTATGGCGGCCATCG
GAGTTAAAACACCCATCAGAGGTAAACCATTCTTACCACTCCTTACACCCTCTTAAGCTC
CTCTCAGGTCAATTACCGGATAATTGTGATGGAAAATGTCGTCTTTGCGCAAGAAAGATT
GATGATAGATTGTTCTATCATTGTTCTCCGTGCAACTTCACCTTGGATCTACGTTGTGTT
TTAAACCCACCACAACAATCTCTTCTGAATTTGAAAGCTCATGATCATCAACTCACCCTT
CTCCCAAGACTCTTGTCATTTACATGTAATGCTTGCGGGTTGAATGGAGATCGAAGCCCT
TACACTTGTGTTCAATGTGATTTCATGATTCATCAAGATTGTCTTGACTTACCACGCGTC
ATAAACATTAATCAGCATGATCACCGTGTTTCTCGAACTTCTGTTCCTGGTGTTGGAAAT
TCTAAGGGAGTGTTACTACCGTGGTTTCACAGGAAGCATATAATGATGCCTTGGAATGAT
ATGAGGAAAGGAGATTGTTGTGGAAGTTTGGAATCTGTCACTGACGGCTTCTATTGCAAA
AGCTGCGATATTTTCATCCACAAGAAATGTGGCGAGTCCTCCGGAATTATCGACCACTCA
TCTCACCCCGATCACACTCTTGAGCTTAACCGTTATCCTAATAAAAGTTGCAATTTATGT
GGAAGGTCCAAAGGTGTGAATGTATGCTATCGTTGTGATCACTGTTACTACCAATTGGAT
CTATATTGTGCCAAGTACCCACCACCAGAGTTTATTGACATTCCCGAAACGCATCACCAC
AAGCTCACCCTTCTCAAGGAGCGGATTGAGTTTGATTGTGATGCTAAATGTGGGGAGACT
GGAGATGGGTTTGCTTACAAATGTCCTGAATGTGATTTATTCTTCCATGTGGATTGCGTA
TGGCATCCGCCAGAAGTAAATCACCCTTTAGAGGTAAACCACTCTTACCACCCCTGGCAC
CCTCTTAAGCTCCACACAGGTCAACCACCACACTATTCTGATGGAACATGTCGTCTTTGT
GCAAGAAATATTGATGATAGATTGTTTTATCATTGCTCTCCGTGCAACTTCACATTGGAT
CTGCGTTGTGTTTTAAATCCACCGCCACAATCTCTTTTGAATTTGAAAGCTCATGACCAC
CAACTCATCCTTCTCCCAAGACTCCGTTCTTTTACATGTAACGCTTGCGGGTTGAGTGGT
GATCGAAGCCCTTACATATGTATTCAATGTGACTTTATGATCCATCAAGATTGTCTTGAC
CTTCCACGCCTCATAAACGTTAATCGTCATGATCACCGAGTTTCACGAACTTCTGTTCTT
GGTGTTGTAAATTCTGTATGTGGAGTTTGTCATCAGAAGGTGGATTGGACTTGTGGAGGT
TTTTCTTGTCAGAGGTGTTCTAGCTATGTTGTTCATTCGAAATGTGCTACCAGAAGAGAT
GTATGGAACGGGAAAGAACTGGAAGGAGTACCTGAAGAAATAGAAGATATAGAGCCATAT
GTGGTAATAGATGACAATACAATACAACATTTCAGCCACAAAGAGCATTACCTAAGACTC
CACGTTAATGATGTTCTATGTAACGACAACAAGCGGTGCAAGGCATGCACCCATCCCATC
TTTCTTCAATCCTTCTACAGTTGTATGGATTGTGACTTCCTTCTCCACCAAAATTGCGCT
GGATTTCCTAGAATGAAAAGGCATGTGCTACACAATGAGCGTCTTACTTTAGTTACTAAC
GAGACTAAACTCTTTCAGTGTGCTCCTTGTGATAGATGGTCCAATGGTTTCAGGTACCAA
CATGGATATAAGTCACTCGATCTTCAGTGTGGTTCAATATCCGAGCCATTTGTCCATCCA
AGCCATCCCGATCATCCCTTATATCACACTTTACTGGATGGAAGGAATGAGATTTGTGAT
GGCTGCAAAAAGAGTTGGTATTATGTGCTAAGTTGCATTGAAGATGATTGTAGATTTGTC
TTATGTTTCAAATGCCTCACTTTACCACAAGTGGTAAAGCATAGAGTTGACGATGATCCT
CTCTTGTTATGCTATGGCGAAAAGGCTTGTGGTAAATACTGGTGTGATATTTGTGAAAAG
GAAACCAATCCAGAGACATGGTTCTACACGTGTAAAGATCATCAATCTAGTATGCATACA
ATGTGTGTGCTTGGTGACTCTGTAGGGATCATGCCAAAAAGCACAATAATGGGTTGGTAC
AAATATTATGAGGTGGTGCTCAATAATAGTATATCTCGTCCAGTTTGCGAGATGTGCAAG
TCGCGTTGCATTTTTCCTACGATCATGAAGATTCTTGAAGCCTCAAATGAATACGTTTGC
TCTTTAGCATGCGTCCTAGAGAGGGGGTGA

Protein sequence

MGLINQWFPLDTLATPERIRMACFDEFTSPFYMTMDSEGVLMPLVHEHLMMPWNDLRKGD
CCGLSKAVSVGYYCKSCDFFAHKKCGESSEFIQHPSHPNHTLQLRSSEGCNSCNLCGRTI
SDLFYRCDLCDFDVDLYCAKYPPPEVIDIPETHHHKLNLHKELIEFNCDAKCGKITGAEF
PYVCYECQLPFHVDCVWRPSELKHPSEVNHSYHSLHPLKLLSGQLPDNCDGKCRLCARKI
DDRLFYHCSPCNFTLDLRCVLNPPQQSLLNLKAHDHQLTLLPRLLSFTCNACGLNGDRSP
YTCVQCDFMIHQDCLDLPRVININQHDHRVSRTSVPGVGNSKGVLLPWFHRKHIMMPWND
MRKGDCCGSLESVTDGFYCKSCDIFIHKKCGESSGIIDHSSHPDHTLELNRYPNKSCNLC
GRSKGVNVCYRCDHCYYQLDLYCAKYPPPEFIDIPETHHHKLTLLKERIEFDCDAKCGET
GDGFAYKCPECDLFFHVDCVWHPPEVNHPLEVNHSYHPWHPLKLHTGQPPHYSDGTCRLC
ARNIDDRLFYHCSPCNFTLDLRCVLNPPPQSLLNLKAHDHQLILLPRLRSFTCNACGLSG
DRSPYICIQCDFMIHQDCLDLPRLINVNRHDHRVSRTSVLGVVNSVCGVCHQKVDWTCGG
FSCQRCSSYVVHSKCATRRDVWNGKELEGVPEEIEDIEPYVVIDDNTIQHFSHKEHYLRL
HVNDVLCNDNKRCKACTHPIFLQSFYSCMDCDFLLHQNCAGFPRMKRHVLHNERLTLVTN
ETKLFQCAPCDRWSNGFRYQHGYKSLDLQCGSISEPFVHPSHPDHPLYHTLLDGRNEICD
GCKKSWYYVLSCIEDDCRFVLCFKCLTLPQVVKHRVDDDPLLLCYGEKACGKYWCDICEK
ETNPETWFYTCKDHQSSMHTMCVLGDSVGIMPKSTIMGWYKYYEVVLNNSISRPVCEMCK
SRCIFPTIMKILEASNEYVCSLACVLERG*

Gene	T10M13.18
Putative Identification	hypothetical protein
Position	93637 to 95616, from the initial methionine to the termination codon
Strand	+
EST match	none
Database match	several Arabidopsis hypothetical proteins

CDS: The table below lists the coordinates of the T10M13.18 exons and which algorithms selected the particular termini (GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene).

Exon	Range	5'	3'
1	93637 - 95616	GS, Gr	GS

Alternate exons not used in building the gene model: The initial exon predicted by GRAIL extends from 93637 to 94468. Other exons extend from 94842 to 95004 and from 95034 to 95308. MZEF, not designed to predict terminal exons, locates no internal exons in this region. NetPlantGene predicts putative splice sites throughout this region. Notable site include splice acceptors at and splice donors at 93832 (0.88), at 93853 (0.92) and at 95512 (0.99).

Complete CDS of T10M13.18

ATGGATTCTGAGGGAGTGTTATTGCGGTTGATCCACGAGCATTCTATGATCCCTTGTAAT
CATCTGAGGAAAGGTGATTGTTGTGGACGCTTTGAAGCTATAAGCGATGGCTACTACTGC
AAAACATGCGATTTTTTCGTCCACAAGAAATGTGTCGATGAGGCCTCTGAATCTATCGAG
CATCCATCTCATCCCGGTCACACTCTTCAGCTTCTAAGTAAACAAAAATATCGTTATTGT
AATTTATGTGGAAGGGACATTAAGGATCTATGCTATCATTTTGGTAACTTCGACGTGGAT
CTATATTGTGCAAAGTACCCACCACCAGAGGTTATTGAAAGTTCTGAGACACCATGCCAC
AAGCGCACCCTTCTCAAGGAGAAGATCGAGTTTCATTGTGATGCTAAATGTGAGGAGATT
GGTTATGGCTTTCCTTACAAATGTCTTGAATGTGGTTTGACCTTTCATATCGACTGTGTT
TTGCATCCATCCGGGGTAAAACACCTGCCAGAGGTAATCCACCCGCTAGACGTAAAGAAT
CACTCTTGCCACCCCTTGCACCCTCTTAAGCTTGTTCTCACAGGTCAACTACCAGACTAT
TCTGATGGAAAATGTCGTCTTTGCGAAAAAAAGATCGATAGCCCTTTGTTCTATCATTGT
TCTCCATGTAACTTCACCTTGGATATGCGTTGTGCTTTAAACCCGCCATCAATATCTTTC
GAGGATTCGAAAACTCATGATCACCAACTCACTCTTCTCCCAAGACTCGACTCTTTTACA
TGTAATGCTTGTGGGCTGAAGGGAGATCGAAGCCCTTACATATGTGTTCAATGTAATTTC
ATCATCCACCAAGAGTGTCTTACCCTTCCACGCCTTATAAACATAAATCGGCATGATCAC
CGTGTTGCTCGCACTAATCTTCTGGGTCTTGTATATTCTGTGTGTGGAGTTTGTCGCCAA
AAAGTGGATTGGACTTGGGGAGGTTATTCTTGTCAAAGGTGTTCTAACTATGTCGTTCAT
TCCAAATGTGCTACAAGAAAAGATGTGTGGAACGGGAAAGAACTCGAAGGAGTTATTGAA
GAAACAGAAGATATAGAGCCGTATGTGGTAATAGATGACAACACAATACAACATTTCAGC
CACGAAGAGCATTACCTAAAACTCGACGACAATGGTGTTTTATGTGATGAAAACAAGCGG
TGCAGCGCATGCACCCATTCCGTTTGCCTCGAATCCTTCTATGGTTGTATGGATTGTGAC
TTCATTCTTCACCAAAACTGTGCTAAATTTCCTAAAAGGAAACGACATGTGCTACACAAT
GAACGGCTTACTTTATTTACACGCGAGGCTGGTCACTTTTGGTGTAATGTTTGTGGTAGA
ATCTCCAATGGTTTCAGTTACCAATATGGGGATATGAAATTAGATGTCATATGTTGTTCA
GTGTTGGAGCCATTTGTCCATCCAAGTCATCCCGATCATCCCTTATATTACATTTCACCA
GAGATGGAAGAAGTCTGCAATGGTTGTAACATGTCGGGAACTAGAATGCTCAGGTGCATT
GAAGATGGTTGTGGATTTGTCTTATGCTTCAAATGCGCCACTTTACCGCGAGTGTTAAAG
CATAGAGTTGACGATTATCCTCTCTTACTATGCTATGGCGAAAAGGCAAATGGTATATAT
TGGTGTGAGATTTGTGAGAAAAAAATGAATCCAGAGAAATGGTTCTACACATGTAAAGAT
CAATGGGCTAGTTTGCACACAGAGTGTGTAGTCGGAGACTTTTCAGGGCTCATGCCAGGA
AGCGTAGTAAAGGCTGAGACGGGATCATATGAGGTTGTGCTCAACAAGAATGTATCTCGC
CCGTTTTGCAGGCAGTGTAAGTCGCATTGCATGTACCCTATCATCTATAAGATACCTGAA
ACCTCAGTTTCTTATCTTTGCTCTGATATTTGCATAAAGCGTTTTACAAAGAGAGACTGA

Protein sequence

MDSEGVLLRLIHEHSMIPCNHLRKGDCCGRFEAISDGYYCKTCDFFVHKKCVDEASESIE
HPSHPGHTLQLLSKQKYRYCNLCGRDIKDLCYHFGNFDVDLYCAKYPPPEVIESSETPCH
KRTLLKEKIEFHCDAKCEEIGYGFPYKCLECGLTFHIDCVLHPSGVKHLPEVIHPLDVKN
HSCHPLHPLKLVLTGQLPDYSDGKCRLCEKKIDSPLFYHCSPCNFTLDMRCALNPPSISF
EDSKTHDHQLTLLPRLDSFTCNACGLKGDRSPYICVQCNFIIHQECLTLPRLININRHDH
RVARTNLLGLVYSVCGVCRQKVDWTWGGYSCQRCSNYVVHSKCATRKDVWNGKELEGVIE
ETEDIEPYVVIDDNTIQHFSHEEHYLKLDDNGVLCDENKRCSACTHSVCLESFYGCMDCD
FILHQNCAKFPKRKRHVLHNERLTLFTREAGHFWCNVCGRISNGFSYQYGDMKLDVICCS
VLEPFVHPSHPDHPLYYISPEMEEVCNGCNMSGTRMLRCIEDGCGFVLCFKCATLPRVLK
HRVDDYPLLLCYGEKANGIYWCEICEKKMNPEKWFYTCKDQWASLHTECVVGDFSGLMPG
SVVKAETGSYEVVLNKNVSRPFCRQCKSHCMYPIIYKIPETSVSYLCSDICIKRFTKRD*

Comparison of T10M13.17.1 and T10M13.18. There are numerous CxxC peptide elements repeated in these proteins. Protein T10M13.17.1 from residues 48 to 344 is 75% identical to residues 353 to 649. The two proteins from T10M13 are aligned to three other hypothetical proteins.

             1                                                         60
T10M13-17.1  MGLINQWFPLDTLATPERIRMACFDEFTSPFYMTMDSEGVLMPLVHEHLMMPWNDLRKGD 
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 

             61                                                       120
T10M13-17.1  CCGLSKAVSVGYYCKSCDFFAHKKCGESSEFIQHPSHPNHTLQLRSSEGCNSCNLCGRTI 
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 

             121                                                      180
T10M13-17.1  SDLFYRCDLCDFDVDLYCAKYPPPEVIDIPETHHHKLNLHKELIEFNCDAKCGKITGAEF 
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 

             181                                                      240
T10M13-17.1  PYVCYECQLPFHVDCVWRPSELKHPSEVNHSYHSLHPLKLLSGQLPDNCDGKCRLCARKI 
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 

             241                                                      300
T10M13-17.1  DDRLFYHCSPCNFTLDLRCVLNPPQQSLLNLKAHDHQLTLLPRLLSFTCNACGLNGDRSP 
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 

             301                                                      360
T10M13-17.1  YTCVQCDFMIHQDCLDLPRVININQHDHRVSRTSVPGVGNSKGVLLPWFHRKHIMMPWND 
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MDSEGVLLRLIH.EHSMIPCNH 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MATTAVNLP.IH.EHPLYPSAR 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MASLRKKLPLNR 

             361                                                      420
T10M13-17.1  MRKGDC..CGSLESVTDGFYCKS..CDIFIHKKC.GESSGIIDHSSHPDHTLE.LNRYPN 
  T10M13-18  LRKGDC..CGRFEAISDGYYCKT..CDFFVHKKCVDEASESIEHPSHPGHTLQLLSKQKY 
    F2N1.27  CIIGECDGCHVNGYMYNGYFCNEPFCFIWFHKECA.EAPAEISHSSHHQHPLLLTNDSID 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  FRRRLDKLRSRHNIRSVRQRFGNGGNTQQQRPPTKHLCPFSDPVEPHKLQRRRLLSQSQL 

             421                                                      480
T10M13-17.1  KSCNLCGRSKGVNVCYRCDHCYYQLDLYCAKYPPPEFIDIPETHHHKLTLLKERIEFDCD 
  T10M13-18  RYCNLCGRDI.KDLCYHFGN..FDVDLYCAKYPPPEVIESSETPCHKRTLLKEKIEFHCD 
    F2N1.27  GPCDLCGQKL.LPPCYSCSTCEFKVDLTCAMKPSPPAIEHPLCHHHSLVFLKKREEKVSC 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSKLMLPFHEHPMTISNLYNHAC 
  F21P24.16  SECFGCKGKHLEGKRYYYFCPLCNLEFHRGCHVLPQQMKHPLHLSHPLTLISLDPNFDT. 

             481                                                      540
T10M13-17.1  AKC.GETGDGFAYKCPECDLFFHVDCVWHP......PEVNHPLEV.NHSYHPWHPLKL.H 
  T10M13-18  AKC.EEIGYGFPYKCLECGLTFHIDCVLHPSGVKHLPEVIHPLDVKNHSCHPLHPLKLVL 
    F2N1.27  ELC.KDSIAGPSYSCLECDVYFHVNCI......QLSKEVNHP.......CHSAHPLKLIP 
  T19K24.12  DFCPGRPKPGTIFFCEKCDLDLHKGCA..GKFLNNSSRCNHSLKIFQGR........... 
  F21P24.16  SKIPQEWGESSS.SDSEGSFDSDDEFGGRRRLLRRRARFFSPFFDVEDDDDDDVDLDDDD 

             541                                                      600
T10M13-17.1  TGQPPHYSD......GTCRLCARNIDDRLFYHCSPCNFTLDLRCVLNPPPQSLLNLKAHD 
  T10M13-18  TGQLPDYSD......GKCRLCEKKIDSPLFYHCSPCNFTLDMRCALNPPSISFEDSKTHD 
    F2N1.27  FESLTDDAE......TTCLLCGKKPAENMLYHCSVCNFTSCLGCTKNPPLLVIEHIKTHK 
  T19K24.12  FGQFGEDDD......YQCHYCQQNVGFH.FARCTICNISIDEKCLRNPPPLTIFQPKHHK 
  F21P24.16  DDDDDDDNDLSDIYYGKCKCCQKRFED.IYYHCSVCNFSLNFTCTIKPPPLTITHLKSHS 

             601                                                      660
T10M13-17.1  HQLILLPRLRSFTCNACGLSGDRS...PYICIQCDFMIHQDCLDLPRLINVNRHDHRVSR 
  T10M13-18  HQLTLLPRLDSFTCNACGLKGDRS...PYICVQCNFIIHQECLTLPRLININRHDHRVAR 
    F2N1.27  HPLILFPRRIPCICDICGKKCEFA...VYVCPQCDFVTARGCIDLARVININRHDHRIYL 
  T19K24.12  HSLFLLGRLVAFTCNACGVEGDRN...PYICIECNLMLHKECTDLPRVISINRHEHRISH 
  F21P24.16  HILTLFPRRIPLPCDVCGLSLNDAHDPVYACLPCNYMVHRACINLPRVIKITRHQHRLSL 

             661                                                      720
T10M13-17.1  TSVLGVVNSV..CGVCHQKVDWTCGGFSCQRC.SSYVVHSKCATRRDVWNGKELEGVPEE 
  T10M13-18  TNLLGLVYSV..CGVCRQKVDWTWGGYSCQRC.SNYVVHSKCATRKDVWNGKELEGVIEE 
    F2N1.27  THHLGTGYSK..CGVCHENVNQYKGAYSCSVC.PHYAVHSTCAVRTDVWDGVELEGTTEI 
  T19K24.12  TFHLGKGEGVWECGVCRKTIDCVYGAFICSRCPSSYAVHSRCATRKEVWDGIELEDVPEE 
  F21P24.16  ISSL..ISGDFSCGVCRKTIDISYGHYSCIK.GCCYGAHSKCATNKNVWDGKDLEDVPEE 

             721                                                      780
T10M13-17.1  IEDIE.PYVVIDD.NTIQHFSHKEHYLRLHVNDV.LCNDNKRCKACTHPIFL.QSFYSCM 
  T10M13-18  TEDIE.PYVVIDD.NTIQHFSHEEHYLKLDDNGV.LCDENKRCSACTHSVCL.ESFYGCM 
    F2N1.27  TEDNS.PFKVVGD.NLICHFSHEEHPLRFQKDDVFIHDEHKRCAACIHPLGF.GSIYCCE 
  T19K24.12  DEELEDPFKVINEKGDIVHFSHEEHVLRLDVNYVH.DNDTMCCGGCILPIND.DPCYKCV 
  F21P24.16  PEEVIEPFVRIDEE.TIHHFTHSCH.LKLQGKGSHR.GEKKFCKACCFPISIYDTFYTCL 

             781                                                      840
T10M13-17.1  DCDFLLHQNCAGFPRMKRHVLHNERLTLVTNETKLF............QCAPCDRWSNGF 
  T10M13-18  DCDFILHQNCAKFPKRKRHVLHNERLTLFTREAGHF............WCNVCGRISNGF 
    F2N1.27  TCPFILHEKCANLPMKKRLVFGTRPYTLMKETTQLT............DCTLCGIFSDGF 
  T19K24.12  ECDFCLHKGCASLPRKKAHFLHNHKISLRAHKDDVY..........VDKCGACKTYSNGF 
  F21P24.16  DCDFVLHEACACLPRIQHHPLHKHPLILNKSDPSLQLYVDGYSVEGMFACSACRRHSTGF 

             841                                                      900
T10M13-17.1  RYQ.......HGYKSLDLQCGSISEPFVHPSHPDHPLYHTLLDGRNEICDGCKKS.WYYV 
  T10M13-18  SYQ.......YGDMKLDVICCSVLEPFVHPSHPDHPLYY.ISPEMEEVCNGCNMS.GTRM 
    F2N1.27  AYS.......SRWLDVDVHCGSLNEPLVHDGH.IHPLNFDKMEG..HFCDGCKKNIDDYM 
  T19K24.12  KYECHDKSSCRGNIKYDIRCSSISEPFHHDDLHPHPLYWTLEETKQ..CKACGTEI.LKP 
  F21P24.16  VYSCNVEFC...DFQVDVKCAFLPDTSVHESHPNHPIYINLTKG...ACMGCSNACSRKY 

             901                                                      960
T10M13-17.1  LSCIEDDCRFVLCFKCLTLPQVVKHRVDDDPLLLCYGEK..ACGKYWCDICEKETNPETW 
  T10M13-18  LRCIEDGCGFVLCFKCATLPRVLKHRVDDYPLLLCYGEK..ANGIYWCEICEKKMNPEKW 
    F2N1.27  LRC..KACDFDLCLYCATLPKKIWHRNDGHPLTLCCEEKEEASDKYWCDICEKELDP... 
  T19K24.12  LSCLV..CEYALCIRCATLPKKVKHRCDDHFLSLCH.DAGNSSGDLWCDICETKTDPSVC 
  F21P24.16  LECLI..CDSFLGVECATLPSVAHYKHDSHPLTLCYGEKGTTSGQYWCEICESKLDASEW 

             961                                                     1020
T10M13-17.1  FYTCKDHQSSMHTMCVLGDSVGIMPKSTIMGWYKYYEVVLNNSISRPVCEMCKSRCIFPT 
  T10M13-18  FYTCKDQWASLHTECVVGDFSGLMPGSVVKAETGSYEVVLNKNVSRPFCRQCKSHCMYPI 
    F2N1.27  MCIRRFLTSRARTKLLVWGKSQVWGGS*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  YYTCDKCGVSLHINCVLGDLYYTKVGLVYPG....LEVLPNNGATRPFCNSCKVRCKFSF 
  F21P24.16  FYTCDSCRVTLHLKCLLGNDMYIKPQHFV.GKEGEIEIALNDGNTRPFCSVCHVRCTDIS 

             1021                                                    1080
T10M13-17.1  IMK.ILEASNEYVCSLACVLERG*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T10M13-18  IYK.IPETSVSYLCSDICIKRFTKRD*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  LMKQTLGDLVRYFCSIRCIPRAR*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  VFKY....QDNVYCDLGCVRKVKIYESRGSELFSSQTAQLKSLKLIRLLDRARKLKDSQR 

             1081                                                    1140
T10M13-17.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  EFKFPLILSLHTKEENLRQDEIIWQREEVSVSGISFSEIPTTRRSVNDETDSLDHVTSQI 

             1141                                                    1200
T10M13-17.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  YNNIKRTLITLDSSDSQGLVHVLMWNDQGLSWNPRVFYLPNFATKQQCEAVIDMAKPKLK 

             1201                                                    1260
T10M13-17.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  PSTLALRKETKHFQMQYRSLHQHTDEDESGVLAAIEEKIALATRFPKDYYESFNILRYQL 

             1261                                                    1320
T10M13-17.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  GQKYDSHYDAFHSAEYGPLISQRVIDSKLPSVDPPLMAQHMNAGRDFPIVLIECRRRWRD 

             1321                                                    1380
T10M13-17.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  F21P24.16  DVPIRGNRDIDKNVETKKKMKKLELGSQIKVSITTPIDPKFTGKSAGERSKRTNKKTQLR 

             1381                   1407
T10M13-17.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~
  T10M13-18  ~~~~~~~~~~~~~~~~~~~~~~~~~~~
    F2N1.27  ~~~~~~~~~~~~~~~~~~~~~~~~~~~
  T19K24.12  ~~~~~~~~~~~~~~~~~~~~~~~~~~~
  F21P24.16  RREFEILTDMFEFTTTQLIAKDVYQI*

Amino acid composition of T10M13.17.1 and T10M13.18

amino acid	T10M13.17.1 # %	T10M13.18 # %
alanine	23 2.3%	15 2.3%
arginine	49 5.0%	31 4.7%
asparagine	46 4.7%	26 3.9%
aspartic acid	71 7.2%	41 6.2%
cysteine	86 8.7%	59 9.0%
glutamine	27 2.7%	13 2.0%
glutamic acid	47 4.8%	43 6.5%
glycine	49 5.0%	37 5.6%
histidine	63 6.4%	38 5.8%
isoleucine	41 4.1%	33 5.0%
leucine	100 10.1%	60 9.1%
lysine	51 5.2%	44 6.7%
methionine	20 2.0%	11 1.7%
phenylalanine	41 4.1%	27 4.1%
proline	62 6.3%	35 5.3%
serine	66 6.7%	42 6.4%
threonine	38 3.8%	26 3.9%
tryptophan	14 1.4%	7 1.1%
tyrosine	39 3.9%	30 4.6%
valine	56 5.7%	41 6.2%
calculated pI	6.89	6.82

written 30 Jul 97
updated 5 Aug 98
Larry Parnell