Gene T10M13.4
Putative Identification predicted protein of unknown function
Position 18527 to 23440, from the initial methionine to the termination codon
Strand +
EST match N65123
Database match C. elegans protein B0414.8

 

CDS:  The table below lists the coordinates of the exons of T10M13.4 and which gene modeling algorithm selected the 5' and 3' termini for each exon (GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene - selects splice sites only, not exons). Splice sites of the CDS determined by comparison to EST N65123 are designated by EST and those determined by similarity to EST AA660863 are designated by est. Alignment with Medicago truncatula EST AA660863 is not at perfect identity to T10M13.4, but can aid in suggesting splice sites. Interestingly, the first ATG codon for which the alignment to the EST is perfect is at position 18569-18571 and this may represent an alternate choice for the initial methionine. This codon and its methionine are highlighted below.

Exon Range 5' 3'
1 18527 - 18697 Gr est, GS, Gr, NPG
2 19163 - 19302 est, GS, Gr, M, NPG est, GS, Gr, M, NPG
3 19428 - 19482 est, GS, Gr, NPG est, GS, Gr, NPG
4 19571 - 19678 est, GS, Gr, M, NPG est, GS, Gr, M, NPG
5 19786 - 19884 est, Gr, NPG Gr, NPG
6 20015 - 20092 GS, Gr, M, NPG GS, Gr, M, NPG
7 20247 - 20324 Gr, M, NPG Gr, NPG
8 20463 - 20615 GS, Gr, M, NPG GS, M, NPG
9 20856 - 20956 Gr, M, NPG Gr, M, NPG
10 21395 - 21461 Gr, NPG Gr
11 21869 - 22123 GS, M, NPG EST, GS, M, NPG
12 22204 - 22320 EST, GS, NPG EST, GS, Gr, NPG
13 22397 - 22468 EST, M, NPG EST, M, NPG
14 22549 - 22599 EST, Gr, M, NPG EST, Gr, NPG
15 22685 - 22921 EST, GS, Gr, M, NPG GS, Gr, M
16 23016 - 23192 GS, Gr, NPG GS, NPG
17 23315 - 23440 GS, Gr, NPG GS, Gr

Alternate exons not used in building the gene model: GenScan concatenates T10M13.3 and T10M13.4 and so does not predict an initial exon for T10M13.4. Alternate GenScan exons are from 18350 to 18481, from 18489 to 18697 and from 18947 to 19078. GRAIL predicts exons from 20463 to 20690, 21237 to 21334, from 21656 to 21787, from 22274 to 22320 and from 23016 to 21395. MZEF predicts exons from 20247 to 20311 and from 22549 to 22566. NetPlantGene does not predict any potential splice sites above a confidence score of 0.90 that were not used in building the gene model.

Complete CDS of T10M13.4

ATGGCGACGGAGGCAGCTCCGATGGACGAAAAGGCGAAGAGAATGAGAGATCTATTGTCG
AGCTTCTACGCACCGGATCCTTCAATCTCGACGAGTGGTTCCTCCATCAACGCCTCTTTC
GATAACATCAACAGCACTTCCTTTGATGCTGATCAGTACATGGATCTCATGATCAAAAAG
TCGAATTTGGAGGTGCTTCTGCAAAGACATGTTCAAATGGCTGCTGAGATTAAAAATCTC
GACACAGACTTGCAAATGCTAGTCTATGAAAATTACAACAAGTTTATCAGTGCAACAGAT
ACAATCAAAAGGATGAAGAGTAATATTTTCGGGATGGAAGGCAATATGGACCAGCTTCTT
CAGAAGATAATGTCAGTACAATCAAAGAGTGATGGGGTCAACACTTCTCTTTTCGAAAAG
AGAGAACATATAGAGAAACTGCACCGGACTCGTAATCTTCTTCGTAAAGTTCAGTTCATC
TATGATTTACCTGCAAGACTGCAAAAATGTATCAAGTCAGAAGCCTATGGCGATGCTGTC
AGGTTCTATACTGGAGCAATGCCAATTCTCAAGGTATATGGCGATACATCATTCCAAGAC
TGCAGGCGAGCTTCCGAAGAAGCTATAGAAATTATCATAAAGAACTTGCAGACGAAGCTA
TTTTCAGATTCAGAATCCATACAGGCGAGAGCTGAAGCTGCAGTGCTTCTTAAGCAGTTA
GATGTCCCTGTTGATAGCTTAAAGGCTAAACTGTTGGAAAAACTGGAACAGTCTCTTGAT
GGTCTTCAAATAAAGCCTGAGGAGGCAAGTACACTTGTAGAGGACGATGATTCATCTAAC
GATACAGAAAGCAATGACCAACATCCTGCTAAAATTCATGAGGATGCCGTACGTGGATTT
TCTGAGGCCATACGTGCTTATCGAGAAATATTCCCCGACTCAGAAGAAAGACTTTTCAAA
CTCGCGAGAGCCTTAACAGCAATGCTGCCCAGGTTACTCTTAAGCAATTTGTTGCGAGAA
TGTTTTCTCATCTTCAACAGGATATATCAGGACTTCCGTCAGCTTCTTGATGAAAAAACT
GGAATATTTATAAAGATGAAGGATTTAATCAGTGGTTGGATTCAGAAAGGATCTCAAGAC
TTCTTCAGGTCACTAGAAGCTCAATTTCTAGTGCTTTCTGGAAAAACTAGCTCATCAAAC
GATATAGAGGGAAAATCGAGTGACAAAATTCATGCTGGTCTCATTCTTGTATTGGCGCAG
CTCTCTGTCTTCATCGAACAAAAGGTCATCCCGAGAGTTACTGAGGAAATAGCTGCTTCC
TTCTCTGGTGGAAATTCCCAAGCCTTTGAGAATGGACCTGCTTTTATTCCTGGAGAACTT
TGCCGGGTCTTCCACGCAGCCAGCGAGAAACTTCTCCAGCATTACATAGACACAAGAACA
CAGAAGGTATCAGTTTTACTGAGAAAAAGGTTCAAGACACCTAACTGGGTTAAGCACAAG
GAGCCACGAGAGGTTCACATGTATGTCGATATGTTTCTTCACGAGTTAGAAGAAGTTGGT
AAAGAAGTGAAACAAGTTTTACCTCAAGGGACTTTCCGTAAGCACAAAAGAACAGACAGC
AACGGAAGTAACACTACAACCTCATCACGAAGCAATACCCTCCATAATGATAAGATGGCA
CGGTCAAATTCACAAAGAGCTCGTAGTCAGCTTTTCGAGACACATCTTGCAAAACTGTTC
AAGCAAAAAGTAGAAATATTCACCAAGGTTGAATTTACCCAGGAATCCGTTGTTACAACA
ACAGTAAAACTTTGTCTGAAGAGTTTGCAAGAATATGTCCGTCTCCAAACGTTTAACCGG
AGCGGGTTCCAGCAGATTCAGCTAGACATTCAGTTCTTAAAAGCTCCATTGAAGGAAGCA
GTTGAGGACGAAGCTGCAATAGACTTCTTGCTCGACGAGGTGATCGTTGCGGCTTCGGAG
AGATGTCTTGATGTGATTCCATTGGAGCCACCGATCTTGGACAAACTCATACAAGCTAAA
CTCGCTAAATCAAAGGAGCACAACAACAACACAGTTTCTTCTTAA

 

Protein translation of T10M13.4

MATEAAPMDEKAKRMRDLLSSFYAPDPSISTSGSSINASFDNINSTSFDADQYMDLMIKK
SNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNMDQLL
QKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAYGDAV
RFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVLLKQL
DVPVDSLKAKLLEKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDAVRGF
SEAIRAYREIFPDSEERLFKLARALTAMLPRLLLSNLLRECFLIFNRIYQDFRQLLDEKT
GIFIKMKDLISGWIQKGSQDFFRSLEAQFLVLSGKTSSSNDIEGKSSDKIHAGLILVLAQ
LSVFIEQKVIPRVTEEIAASFSGGNSQAFENGPAFIPGELCRVFHAASEKLLQHYIDTRT
QKVSVLLRKRFKTPNWVKHKEPREVHMYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDS
NGSNTTTSSRSNTLHNDKMARSNSQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTT
TVKLCLKSLQEYVRLQTFNRSGFQQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASE
RCLDVIPLEPPILDKLIQAKLAKSKEHNNNTVSS*

 

Alignment of T10M13.4 and C. elegans protein B0414.8 

           1                                                         60
 T10M13-4  MATEAAPMDEKAKRMRDLLSSFYAPDPSISTSGSSINASFDNINSTSFDADQYMDLMIKK 
CeB0414.8  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSSVLDVTKPDFDVEAFVVKLLRE 

           61                                                       120
 T10M13-4  SNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNMDQLL 
CeB0414.8  KSLDGLVKEEEEMVSAVRRLDSDVHQIVYENYNKFLTATNTVRKIQDEFTQLDSEMKSLS 

           121                                                      180
 T10M13-4  QKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAYGDAV 
CeB0414.8  RSMSTISTLIGNLDGVLGEKRDDILQLGSSYKVVNSLKHIFDLPHVLRSEFDERNYGEVL 

           181                                                      240
 T10M13-4  RFYTGAMPILKVYGDT.SFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVLLKQ 
CeB0414.8  RMFKLAEESLSQYKDVPTVQLVLQKSKKIYDMTENQLMDQLRNPASGAELVSEAVDLLLT 

           241                                                      300
 T10M13-4  LDVPVDSLKAKLLEKLEQSL..DGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDAV 
CeB0414.8  IGRDEDEVQKVLLTCSEQSLRVDLKELSANHSDVLDLVDKASESFIPNLTLIATTHDRLF 

           301                                                      360
 T10M13-4  RGFSEAIRAYREIFPDSEERLFKLARALTAMLPRLLLSNLLRECFLIFNRIYQ....... 
CeB0414.8  EDKREDLITVLKTEMNSLHAL..VSKVFLSSSDAKDCSIVVRALDRYFRKISTCRYVIPG 

           361                                                      420
 T10M13-4  .DFRQLLDEKTGIFIKMK.DL....ISGWIQKGSQDFFRSLEAQFLVLSGKTSSSNDIEG 
CeB0414.8  LDFLPLTIELINAVSKHEIDLSLTRIKEELKNGLNEVRKALINEEKDLSALASKIEQVFV 

           421                                                      480
 T10M13-4  KSSDKIHAGLILVLAQLSVF.......IEQKVIPRVTEEIAASFSGGNSQ...AFENGPA 
CeB0414.8  HQVKTALANLLLFTASDVTFANLPPDEFRQSFSFNAHERLLVQAFHRFSELADEYESGAG 

           481                                                      540
 T10M13-4  FIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFK............TPNWVKHKEP 
CeB0414.8  EIRFVDPRV.HLVFAVALQHLSNKSAVYLLNLCREQFSLSPDDGLTDITVVMSEVKTRAQ 

           541                                                      600
 T10M13-4  REVHMYVDMFLHELEE..VGKEVKQVLPQGT......FRKHKRTDSNGSNTTTSSRSNTL 
CeB0414.8  KLVRCYAEKTGLSMGETLIKGCAMLVQPAATPSAKFDFRASVRRLVEEMNTCDSELTLLL 

           601                                                      660
 T10M13-4  HNDKMARSN..SQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEY 
CeB0414.8  GGDSKPKDSRVSRRPITTALDAARDSLWCERIDFHLQIHFNRASIITVIVKVVLKIFIES 

           661                                                      720
 T10M13-4  VRLQTFNRSGFQQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPI 
CeB0414.8  IRLQTYSKFGVEQVQVDCYYLQRCLAALVSDEVVVNSMVDQALSSALKRCQDPVLVHPSR 

           721                 743
 T10M13-4  LDKLIQAKLAKSKEHNNNTVSS*
CeB0414.8  LAQLCEQPPANRPSSQASSLGY*

written 29 Jul 97
updated 31 Jul 98
Larry Parnell