Gene T10M13.6
Putative Identification hexose transporter
Position 24652 to 26360, from the initial methionine through the CDS to the termination codon
Strand -
EST match none
Database match hexose (glucose) transporter proteins from fission yeast (Q10710), castor bean (L08197), and sugar cane (L21753).

 

CDS:  The table below lists the coordinates for each exon of T10M13.6 and which gene predicition algorithms select the 3' or 5' exon splice site (GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene - selects splice sites only, not exons).

Exon Range 3' 5'
1 26216 to 26360 Gr, NPG Gr
2 25815 to 26134 GS, Gr, NPG GS, Gr, M, NPG
3 24652 to 25728 GS, Gr GS, Gr, M, NPG

Alternate exons not used in building the gene model:   MZEF selects exons from 24904 to 25728, from 26057 to 26134 and from 27046 to 27204. GenScan selects exons from 26400 to 26467, from 27043 to 27204 and from 27253 to 27368, this latter exon being an initial exon. The only splice site that NetPlantGene predicts with a high confidence score is a splice donor at 27046 (0.92).

Complete CDS of T10M13.6

ATGGCAGGAGGGTCGTTTGGTCCGACCGGTGTGGCTAAGGAGAGAGCAGAACAATACCAA
GGGAAAGTCACTAGTTATGTTATAATCGCTTGCCTTGTTGCAGCTATTGGTGGATCCATC
TTTGGATATGACATTGGAATCTCAGGAGGAGTTACATCGATGGATGAGTTTCTTGAGGAG
TTTTTCCACACGGTTTATGAGAAGAAGAAGCAAGCTCATGAAAGTAATTACTGCAAATAT
GATAATCAAGGCTTAGCTGCTTTCACTTCTTCACTCTACTTAGCTGGTTTGGTTTCGACT
CTCGTGGCTTCACCCATCACTAGGAACTACGGTAGGCGTGCGAGTATTGTTTGTGGTGGA
ATCAGCTTTCTTATTGGATCTGGTTTGAATGCTGGAGCTGTGAACTTAGCTATGCTTCTT
GCCGGACGGATCATGCTTGGTGTTGGTATTGGATTTGGAAATCAGGCGGTTCCTTTGTAT
TTATCAGAAGTGGCACCAACTCATCTACGAGGTGGTTTAAACATGATGTTTCAGCTAGCT
ACAACTATCGGGATCTTTACAGCGAACATGGTTAATTACGGTACTCAACAGCTTAAGCCT
TGGGGATGGAGACTCTCTCTTGGTTTAGCTGCTTTTCCAGCTCTGCTTATGACGCTTGGC
GGGTATTTCTTACCCGAGACACCAAACAGTTTGGTCGAAAGAGGATTAACAGAGAGAGGT
CGACGAGTCTTAGTGAAGCTAAGAGGAACAGAAAACGTCAACGCCGAGCTTCAAGACATG
GTAGATGCGAGTGAGCTTGCGAATTCCATCAAACATCCATTCAGAAACATCTTGCAGAAA
CGACACAGGCCTCAGCTAGTTATGGCTATTTGCATGCCAATGTTTCAGATACTCACAGGG
ATAAACTCCATTCTCTTCTACGCACCTGTTCTGTTCCAGACAATGGGGTTTGGAGGAAAC
GCATCTCTCTATTCATCAGCTTTAACAGGAGCTGTTCTTGTCCTATCAACATTTATTTCC
ATAGGATTAGTAGACAGATTGGGACGAAGAGCTCTTCTCATAACTGGAGGAATACAAATG
ATAATCTGTCAAGTCATAGTAGCAGTGATCTTAGGAGTGAAATTTGGAGACAACCAAGAG
CTATCAAAAGGGTACTCAGTGATCGTAGTCATCTTCATCTGCCTCTTTGTTGTAGCGTTC
GGATGGTCATGGGGTCCTCTAGGTTGGACCATACCTAGCGAAATCTTTCCGTTAGAGACT
CGTTCAGCGGGGCAGAGTATCACAGTAGCTGTAAATCTCCTCTTCACTTTCATCATAGCT
CAAGCTTTCCTTGGTCTCCTCTGTGCATTCAAGTTTGGAATCTTCCTCTTCTTTGCTGGT
TGGGTGACAGTAATGACTATCTTTGTGTATTTCCTGTTGCCTGAAACCAAAGGAGTTCCA
ATAGAAGAGATGACACTCTTATGGAGTAAACATTGGTTCTGGAAAAAAGTATTACCTGAT
GCAACAAATCTTGAAGATGAGAGCAAGAATGTATCTGTTTAA

 

Protein translation:

MAGGSFGPTGVAKERAEQYQGKVTSYVIIACLVAAIGGSIFGYDIGISGGVTSMDEFLEE
FFHTVYEKKKQAHESNYCKYDNQGLAAFTSSLYLAGLVSTLVASPITRNYGRRASIVCGG
ISFLIGSGLNAGAVNLAMLLAGRIMLGVGIGFGNQAVPLYLSEVAPTHLRGGLNMMFQLA
TTIGIFTANMVNYGTQQLKPWGWRLSLGLAAFPALLMTLGGYFLPETPNSLVERGLTERG
RRVLVKLRGTENVNAELQDMVDASELANSIKHPFRNILQKRHRPQLVMAICMPMFQILTG
INSILFYAPVLFQTMGFGGNASLYSSALTGAVLVLSTFISIGLVDRLGRRALLITGGIQM
IICQVIVAVILGVKFGDNQELSKGYSVIVVIFICLFVVAFGWSWGPLGWTIPSEIFPLET
RSAGQSITVAVNLLFTFIIAQAFLGLLCAFKFGIFLFFAGWVTVMTIFVYFLLPETKGVP
IEEMTLLWSKHWFWKKVLPDATNLEDESKNVSV*

 

Protein motifs:

     Two signatures for sugar transport proteins are found in T10M13.6. These are highlighted in the alignment below.

sequence:   340 SIGLVDRLGRRALLIT 355

sequence:   145 MLGVGIGFGNQAVPLYLSEVAPTHLR 170

 

Multiple sequence alignment of T10M13.6 with other hexose transporter proteins:

              1                                                         60
    S. pombe  MAGGSLAPAGVAKERAEQYQGKVTFAVFVACMVAAVGGSIFGYDIGISGGVISMDAFLEK 
 Castor bean  MAGGSLAPAGVAKERAEQYQGKVTFAVFVACMVAAVGGSIFGYDIGISGGVISMDAFLEK 
 Arabidopsis  MAGGSFGPTGVAKERAEQYQGKVTSYVIIACLVAAIGGSIFGYDIGISGGVTSMDEFLEE 
  Sugar cane  MAGGGMAALGVKTERAAQYKGRMTLAVAMTCLVAAVGGAIFGYDIGISGGVTSMDPFLEK 

              61                                                       120
    S. pombe  FFRSVYLKKKHAHENNYCKYDDQRLAAFTSSLYLAGLAASLVAGPITRIYGRRASIISGG 
 Castor bean  FFRSVYLKKKHAHENNYCKYDDQRLAAFTSSLYLAGLAASLVAGPITRIYGRRASIISGG 
 Arabidopsis  FFHTVYEKKKQAHESNYCKYDNQGLAAFTSSLYLAGLVSTLVASPITRNYGRRASIVCGG 
  Sugar cane  FFPVVFHRKNSGGKNNYCKYDNQGLAAFTSSLYLAGLVASLVASPVTRNYGRKASIVCGG 

              121                                                      180
    S. pombe  ISFLIGAALNATAINLAMLLLGRIMLGVGIGFGNQAVPLYLSEMAPTHLRGGLNIMFQLA 
 Castor bean  ISFLIGAALNATAINLAMLLLGRIMLGVGIGFGNQAVPLYLSEMAPTHLRGGLNIMFQLA 
 Arabidopsis  ISFLIGSGLNAGAVNLAMLLAGRIMLGVGIGFGNQAVPLYLSEVAPTHLRGGLNMMFQLA 
  Sugar cane  VSFLIGAALNVAAVNLAMLILGRIMLGVGIGFGNQAVPLYLSEMAPAHLRGGLNIMFQLA 

              181                                                      240
    S. pombe  TTSGIFTANMVNYGTHKLESWGWRLSLGLAAAPALLMTIGGLLLPETPNSLIEQGLHEKG 
 Castor bean  TTSGIFTANMVNYGTHKLESWGWRLSLGLAAAPALLMTIGGLLLPETPNSLIEQGLHEKG 
 Arabidopsis  TTIGIFTANMVNYGTQQLKPWGWRLSLGLAAFPALLMTLGGYFLPETPNSLVERGLTERG 
  Sugar cane  TTLGIFTANLINYGTQNIKPWGWRLSLGLAAAPALLMTLAGLFLPETPNSLIERGRVEEG 

              241                                                      300
    S. pombe  RNVLEKIRGTKHVDAEFQDMLDASELANSIKHPFRNILEKRNRPQLVMAIFMPTFQILTG 
 Castor bean  RNVLEKIRGTKHVDAEFQDMLDASELANSIKHPFRNILEKRNRPQLVMAIFMPTFQILTG 
 Arabidopsis  RRVLVKLRGTENVNAELQDMVDASELANSIKHPFRNILQKRHRPQLVMAICMPMFQILTG 
  Sugar cane  RRVLERIRGTADVDAEFTDMVEASELANTIEHPFRNILEPRNRPQLVMAVCMPAFQILTG 

              301                                                      360
    S. pombe  INIILFYAPPLFQSMGFGGNAALYSSAVTGAVLCSSTFISIATVDRLGRRFLLISGGIQM 
 Castor bean  INIILFYAPPLFQSMGFGGNAALYSSAVTGAVLCSSTFISIATVDRLGRRFLLISGGIQM 
 Arabidopsis  INSILFYAPVLFQTMGFGGNASLYSSALTGAVLVLSTFISIGLVDRLGRRALLITGGIQM 
  Sugar cane  INSILFYAPVLFQSMGFGGNASLYSSVLTGAVLFSSTLISIGTVDRLGRRKLLISGGIQM 

              361                                                      420
    S. pombe  ITCQVIVAIILGVKFGDNQQLSKSFSVLVVIMICLFVLAFGWSWGPLGWTVPSEIFPLET 
 Castor bean  ITCQVIVAIILGVKFGDNQQLSKSFSVLVVIMICLFVLAFGWSWGPLGWTVPSEIFPLET 
 Arabidopsis  IICQVIVAVILGVKFGDNQELSKGYSVIVVIFICLFVVAFGWSWGPLGWTIPSEIFPLET 
  Sugar cane  IVCQVIVAVILGAKFGADKQLSRSYSIAVVVVICLFVLAFGWSWGPLGWTVPSEIFPLET 

              421                                                      480
    S. pombe  RSAGQSITVAVNLFFTFVIAQSFPSLLCAFKFGIFLFFAGWVTVMTAFVYIFLPETKGVP 
 Castor bean  RSAGQSITVAVNLFFTFVIAQSFPSLLCAFKFGIFLFFAGWVTVMTAFVYIFLPETKGVP 
 Arabidopsis  RSAGQSITVAVNLLFTFIIAQAFLGLLCAFKFGIFLFFAGWVTVMTIFVYFLLPETKGVP 
  Sugar cane  RSAGQSITVAVNLLFTFAIAQAFLSLLCAFKFGIFLFFAGWITVMTVFVCVFLPETKGVP 

              481                                    522
    S. pombe  IEEMIFLWRKHWFWKKIVPGQPEVDDSRESMEMGEAVASRIK 
 Castor bean  IEEMIFLWRKHWFWKKIVPGQPEVDDSRESMEMGEAVASRIK 
 Arabidopsis  IEEMTLLWSKHWFWKKVLPDATNLEDESKNVSV 
  Sugar cane  IEEMVLLWRKHWFWKKVMPVDMPLEDGWGAAPASNNHK

written 29 Jul 97
updated 29 Jul 98
Larry Parnell