| Gene | T10M13.7 |
| Identification | PROLIFERA, PRL |
| Position | 27653 to 31744, 5'-UTR through coding region to 3'-UTR |
| Strand | + |
| EST match | none |
| Database match | A. thaliana cv Landsberg erecta PROLIFERA cDNA (L39954) |
| Genetic mapping | PROLIFERA has been mapped to genetic marker GT148, at a position of 11.6 cM from the top arm of chromosome IV. |
Research published by Springer PS, et al. (1995, Science 268:877-880) describes the PROLIFERA (PRL) gene as required for megagametophyte and embryo development. PRL was identified by gene trap transposon mutagenesis and is related to the MCM2-3-5 family of yeast genes required for the initiation of DNA replication. Heterozygotes for disruption of PRL are semi-sterile; siliques contain mutant embryo sacs arrested at various stages: unfertilized ovules and aborted embryos. This suggested reduced viability of the megagametophyte and was confirmed by reciprocal crosses to wild-type plants. A 2.7 kb transcript was detected in RNA from roots, buds, and flowers, while GUS staining indicated that PRL is expressed in dividing cells throughout the plant. For further details, please see the article by Springer PS et al. (1995).
Graphical view of the exon structure of the PROLIFERA gene.
mRNA: Based on a comparison of T10M13 and PROLIFERA cDNA L39954, the 5'-UTR sequence extends from 27653 to 27748 and the 3'-UTR extends from 31563 to 31744.
CDS: The table below lists the coordinates of the 15 exons encoding the PROLIFERA gene and which gene predicting algorithms selected the 5' and 3 termini of each exon (Fe = FEXA, Fg = FGENEA, GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene - selects splice sites only, not exons)
| Exon | Range | 5' | 3' |
|---|---|---|---|
| 1 | 27749 to 27779 | Fe, Fg, GS, Gr | Fe, Fg, GS, Gr, NPG |
| 2 | 27871 to 27938 | Fe, Fg, GS, Gr, M, NPG | Fe, Fg, GS, Gr, M, NPG |
| 3 | 28042 to 28101 | Fe, Fg, Gr, M, NPG | Fe, Fg, Gr, M, NPG |
| 4 | 28190 to 28413 | GS, Gr, M, NPG | GS, M, NPG |
| 5 | 28501 to 28678 | GS, Gr, M | Fe, Fg, GS, Gr, M, NPG |
| 6 | 28766 to 28888 | Gr, NPG | Gr |
| 7 | 28969 to 29058 | Fe, Fg, GS, Gr, M, NPG | Fe, Fg, GS, Gr, M, NPG |
| 8 | 29318 to 29460 | GS, Gr, M, NPG | GS, Gr, M |
| 9 | 29611 to 29788 | GS, Gr, M, NPG | GS, Gr, M, NPG |
| 10 | 30082 to 30273 | Fe, Fg, GS, Gr, M, NPG | Fe, Fg, GS, M, NPG |
| 11 | 30367 to 30539 | Fe, Fg, GS, Gr, M, NPG | Fe, Fg, GS, Gr, M, NPG |
| 12 | 30633 to 30836 | Fe, Fg, GS, M, NPG | Fe, Fg, GS, Gr, M, NPG |
| 13 | 30913 to 31078 | GS, Gr, M, NPG | GS, M, NPG |
| 14 | 31157 to 31378 | Fe, GS, Gr, M, NPG | Fe, GS, Gr, M, NPG |
| 15 | 31464 to 31562 | Gr | Gr |
Complete CDS of PROLIFERA
ATGAAAGACCACGATTTCGACGGCGACAAAGGGCTTGCCAAGGGATTTCTCGAGAATTTC GCCGACGCTAATGGCAGATCTAAGTACATGGAGATTCTCCAAGAGGTTTCAAATCGTAAG ATTCGAGCTATTCAAGTCGATCTCGATGACTTATTCAATTATAAAGATGAAAGTGAGGAG TTTCTCGGACGGTTGACGGAAAACACTCGCCGATATGTTTCAATTTTCTCCGCCGCTGTT GACGAGTTGTTACCCGAACCAACGGAAGCGTTTCCAGATGATGATCATGATATTCTCATG ACTCAGAGAGCAGATGATGGGACTGATAATCCAGATGTTTCTGATCCTCATCAGCAGATT CCTTCTGAGATTAAAAGATATTATGAGGTTTATTTTAAAGCTCCTTCAAAGGGAAGGCCA TCTACCATCAGGGAGGTTAAGGCTTCACACATTGGGCAACTTGTTAGGATATCTGGTATT GTGACCCGTTGTTCCGATGTCAAGCCATTGATGGCGGTTGCTGTTTACACATGTGAAGAT TGTGGTCATGAGATTTACCAGGAAGTTACATCTAGAGTCTTCATGCCTTTGTTTAAGTGT CCTTCTAGTCGCTGTCGTCTTAATAGTAAAGCCGGGAATCCTATTCTTCAGCTCAGAGCA TCAAAGTTTCTAAAGTTTCAGGAGGCCAAGATGCAAGAGTTGGCTGAACATGTTCCCAAA GGGCATATTCCGAGGTCTATGACTGTTCATCTAAGAGGAGAGTTGACAAGAAAGGTATCA CCAGGTGATGTTGTTGAGTTCTCAGGGATTTTTCTTCCCATTCCTTACACTGGGTTTAAG GCACTTCGAGCTGGCTTAGTAGCAGACACCTATCTGGAAGCAACGTCTGTTACTCATTTC AAGAAGAAATATGAGGAATATGAGTTTCAGAAAGATGAGGAAGAACAGATTGCACGTTTG GCTGAGGATGGTGACATATACAATAAGCTGTCGCGATCCCTGGCCCCTGAGATTTATGGA CATGAAGATATTAAGAAAGCTTTGCTTCTCCTTCTTGTGGGTGCTCCTCACAGGCAATTA AAGGATGGAATGAAGATTAGAGGAGATGTGCATATCTGTCTGATGGGTGATCCTGGAGTT GCCAAGAGTCAGCTTCTCAAGCACATTATAAATGTTGCTCCAAGGGGAGTCTACACAACA GGCAAAGGTAGCAGTGGAGTTGGTCTAACTGCCGCTGTTATGAGAGATCAAGTAACAAAT GAGATGGTTTTAGAAGGAGGAGCACTGGTGCTGGCTGACATGGGAATCTGTGCAATTGAT GAGTTTGATAAGATGGATGAATCAGATCGTACTGCTATTCATGAGGTCATGGAGCAGCAA ACTGTAAGCATTGCAAAAGCTGGGATTACCACGTCTTTGAATGCTCGAACAGCTGTTCTT GCTGCAGCCAATCCAGCCTGGGGAAGATATGATTTGCGAAGAACTCCAGCTGAAAACATT AATCTTCCACCGGCCCTTCTTTCAAGATTCGATCTGCTGTGGTTGATCTTAGATCGAGCT GACATGGACAGTGACCTTGAATTGGCAAAGCATGTGCTTCACGTACATCAGACAGAAGAA TCTCCTGCGCTTGGTTTCGAGCCTCTGGAACCCAACATTCTCCGTGCATACATTTCAGCT GCAAGGAGATTATCACCATACGTCCCAGCAGAGTTGGAGGAATATATAGCAACTGCTTAT TCCAGCATCAGGCAAGAAGAAGCGAAATCAAATACTCCTCATTCTTACACAACAGTGAGG ACACTGCTCAGCATTCTGCGCATATCCGCTGCATTAGCAAGGTTGAGATTTTCAGAATCA GTGGCACAGAGTGATGTGGATGAGGCGCTTAGACTAATGCAGATGTCGAAAATATCATTG TATGCTGATGACCGACAAAAAGCAGGACTAGATGCAATATCAGACACATACTCAATCATA AGAGACGAAGCTGCAAGATCAAAAAAGACCCATGTGAGCTACGCAAATGCCCTGAACTGG ATCTCCAGAAAGGGATACAGTGAGGCTCAGCTAAAGGAGTGTTTGGAGGAATATGCAGCT TTGAATGTTTGGCAGATTGATCCTCACACCTTTGACATCCGTTTTATCTGA
Protein translation:
MKDHDFDGDKGLAKGFLENFADANGRSKYMEILQEVSNRKIRAIQVDLDDLFNYKDESEE FLGRLTENTRRYVSIFSAAVDELLPEPTEAFPDDDHDILMTQRADDGTDNPDVSDPHQQI PSEIKRYYEVYFKAPSKGRPSTIREVKASHIGQLVRISGIVTRCSDVKPLMAVAVYTCED CGHEIYQEVTSRVFMPLFKCPSSRCRLNSKAGNPILQLRASKFLKFQEAKMQELAEHVPK GHIPRSMTVHLRGELTRKVSPGDVVEFSGIFLPIPYTGFKALRAGLVADTYLEATSVTHF KKKYEEYEFQKDEEEQIARLAEDGDIYNKLSRSLAPEIYGHEDIKKALLLLLVGAPHRQL KDGMKIRGDVHICLMGDPGVAKSQLLKHIINVAPRGVYTTGKGSSGVGLTAAVMRDQVTN EMVLEGGALVLADMGICAIDEFDKMDESDRTAIHEVMEQQTVSIAKAGITTSLNARTAVL AAANPAWGRYDLRRTPAENINLPPALLSRFDLLWLILDRADMDSDLELAKHVLHVHQTEE SPALGFEPLEPNILRAYISAARRLSPYVPAELEEYIATAYSSIRQEEAKSNTPHSYTTVR TLLSILRISAALARLRFSESVAQSDVDEALRLMQMSKISLYADDRQKAGLDAISDTYSII RDEAARSKKTHVSYANALNWISRKGYSEAQLKECLEEYAALNVWQIDPHTFDIRFI*
Protein motifs:
The MCM2/3/5 family signature is found in proteins required for the initiation of DNA replication in eukaryotes.
sequence: 439 IDEFDKM 445
written 29 Jul 97
updated 30 Jul 97
updated 18 Nov 97
Larry Parnell