Gene:   T10P11.11
Putative Identification:   tryptophan synthase alpha 1-like
Position:   61877 to 63457, initial methionine to termination codon
Strand:   -
EST match:   F20005
Database match:   A. thaliana tryptophan synthase alpha chain (TSA1, U18993)

T10P11.11 is also very similar to B. rapa EST L38526


mRNA:   Based on a comparison to EST F20005, the 5'-UTR sequence is from
        63433 to 63457.

CDS:				exon terminus predicted by	Gr=Grail
					5'		3'	M=MZEF
	exon 1	63358 to 63432		EST,Gr		EST,Gr
	exon 2	63114 to 63260		M		EST
	exon 3	62978 to 63043		M,Gr		M
	exon 4	62716 to 62824		M,Gr		M,Gr
	exon 5	62566 to 62582				Gr
	exon 6	62334 to 62486		Gr		Gr
	exon 7	61877 to 62014		Gr		Gr

ATGGATCTTCTCAAGACTCCTTCCTCCACGGTTGGTCTATCAGAAACATTCGCTAGGTTA
AAGTCACAAGGCAAAGTGGCTCTGATTCCATATATCACAGCTGGTGATCCTGATCTTTCT
ACAACAGCGAAAGCTCTTAAAGTGCTTGATTCGTGTGGCTCCGACATAATCGAACTCGGT
GTTCCATACTCTGATCCATTAGCTGATGGTCCAGCAATCCAGGCTGCTGCGAGACGTTCG
TTACTTAAAGGAACCAACTTTAACTCCATTATCTCTATGCTTAAAGAGGTTATTCCTCAG
TTATCTTGTCCAATTGCATTGTTTACATATTACAATCCAATCCTGCGACGAGGAGTAGAG
AACTACATGACTGTTATAAAGAATGCTGGAGTTCATGGGCTTCTGGTTCCTGATCTGTCA
ATAAAAGATAGCATCTTTCACAGCAAACTGTTTTCTTTCCTTTACCATCTTTACTATGTT
GTCCTCAAGGTACTGCTCACAACACCCACAACTCCAAAAGAACGAATGAATGCCATTGTT
GAAGCCTCTGAAGGATTTATCTATCTCGTAGCTGAATGGGGAGCAGACGGAGTCATTGTT
GGTAGCGCGATGGTTAAGATCTTGGGCGAGTCTGAATCACCTGAGCAAGGACTTAAGGAG
CTAGAATTCTTCACTAAATCTTTGAAGTCTGCTCTTGTCTCTTGA


Protein Translation:

MDLLKTPSSTVGLSETFARLKSQGKVALIPYITAGDPDLSTTAKALKVLDSCGSDIIELG
VPYSDPLADGPAIQAAARRSLLKGTNFNSIISMLKEVIPQLSCPIALFTYYNPILRRGVE
NYMTVIKNAGVHGLLVPDLSIKDSIFHSKLFSFLYHLYYVVLKVLLTTPTTPKERMNAIV
EASEGFIYLVAEWGADGVIVGSAMVKILGESESPEQGLKELEFFTKSLKSALVS*


Protein motif:

Tryptophan synthase alpha chain signature is found from residues 57 to 70.

sequence:   57  IELGVPYSDPLADG  70


Alignment of TSA1 (top) and T10P11.11

                  .         .         .         .         .
       1 MAIAFKSGVFFLQSPKSQIGFRHSSPPDSSLSFKRFTPMASLSTSSPTLG 50
                                               |  | | | |.|
       1 ......................................MDLLKTPSSTVG 12
                  .         .         .         .         .
      51 LADTFTQLKKQGKVAFIPYITAGDPDLSTTAEALKVLDACGSDIIELGVP 100
         |.:|| .|| ||||| |||||||||||||||.||||||.|||||||||||
      13 LSETFARLKSQGKVALIPYITAGDPDLSTTAKALKVLDSCGSDIIELGVP 62
                  .         .         .         .         .
     101 YSDPLADGPVIQAAATRSLERGTNLDSILEMLDKVVPQISCPISLFTYYN 150
         ||||||||| ||||| ||| :||| .||: || .|:||:||||.||||||
      63 YSDPLADGPAIQAAARRSLLKGTNFNSIISMLKEVIPQLSCPIALFTYYN 112
                  .         .         .         .         .
     151 PILKRGLGKFMSSIRAVGVQGLVVPDVPLEETEMLRK.....EALNNDIE 195
         |||:||.  :|. |:  || ||.|||. :.:.    |       |   : 
     113 PILRRGVENYMTVIKNAGVHGLLVPDLSIKDSIFHSKLFSFLYHLYYVVL 162
                  .         .         .         .         .
     196 LVLLTTPTTPTERMKLIVDASEGFIYLVSSIGVTG.....ARSSVSGKVQ 240
          ||||||||| |||  ||:|||||||||.  |  |     |   : |. :
     163 KVLLTTPTTPKERMNAIVEASEGFIYLVAEWGADGVIVGSAMVKILGESE 212
                  .         .         .         .         .
     241 SLLKDIKEATDKPVAVGFGISKPEHVKQIAGWGADGVIVGSAMVKLLGDA 290
         |  . :||      ..   :                              
     213 SPEQGLKELEFFTKSLKSALVS*........................... 235

created 11 Aug 97
Larry Parnell