Gene: T10P11.12
Putative Identification: Mlo homolog
Position: 63938 to 66953, from initial methionine to termination codon
Strand: -
EST match: T22145 and T22146
Database match: Barley Mlo protein, Z83834
CDS: exon terminus predicted by Gr=Grail
5' 3' M=MZEF
exon 1 66746 to 66953
exon 2 66024 to 66277 M
exon 3 65805 to 65921 M M
exon 4 65649 to 65709 M M
exon 5 65481 to 65578 M M
exon 6 65081 to 65219 EST,M M
exon 7 64927 to 64967 EST EST
exon 8 64618 to 64827 M EST,M
exon 9 64236 to 64370 M
ATGGGTCACGGAGGAGAAGGGATGTCGCTTGAATTCACTCCGACGTGGGTCGTCGCCGGA
GTTTGTACGGTCATCGTCGCGATTTCACTGGCGGTGGAGCGTTTGCTTCACTATTTCGGT
ACTGTTCTTAAGAAGAAGAAGCAAAAACCCCTTTACGAAGCCCTTCAAAAGGTTAAAGAA
GGTACCGTACACACACACACACACCAACAGCTGATGTTGTTAGGGTTTATATCGCTGTTA
CTGACGGTATTCCAAGGGCTCATTTCCAAATTCTGTGTGAAAGAAAATGTGCTTATGCAT
ATGCTTCCATGTTCTCTCGATTCAAGACGAGAAGCTGGGGCAAGTGAACATAAAAACGTT
ACAGCAAAAGAACATTTTCAGACTTTTTTACCTATTGTTGGAACCACTAGGCGTCTACTT
GCTGAACATGCTGCTGTGCAAGTTGGTTACTGTAGCGAAAAGGGTAAAGTACCATTGCTT
TCGCTTGAGGCATTGCACCATCTACATATTTTCATCTTCGTCCTCGCCATATCCCATGTG
ACATTCTGTGTCCTTACCGTGATTTTTGGAAGCACAAGGATTCACCAATGGAAGAAATGG
GAGGATTCGATCGCAGATGAGAAGTTTGACCCCGAAACAGCTCTCAGGAAAAGAAGGGTC
ACTCATGTACACAACCATGCTTTTATTAAAGAGCATTTTCTTGGTATTGGCAAAGATTCA
GTCATCCTCGGATGGACGACACATTGTAAGGGAAACCCCAAGCTTAATTTCCACAAGTAT
ATGATGCGCGCTCTAGAGGATGATTTCAAACAAGTTGTTGGTATTAGTTGGTATCTTTGG
ATCTTTGTCGTCATCTTTTTGCTGCTAAATGTTAACGGATGGCACACATATTTCTGGATA
GCATTTATTCCCTTTGCTTTGCTTCTTGCTGTGGGAACAAAGTTGGAGCATGTGATTGCA
CAGTTAGCTCATGAAGTTGCAGAGAAACATGTAGCCATTGAAGGAGACTTAGTGGTGAAA
CCCTCAGATGAGCATTTCTGGTTCAGCAAACCTCAAATTGTTCTCTACTTGATCCATTTT
ATCCTCTTCCAGAATGCTTTTGAGATTGCGTTTTTCTTTTGGATTTGGGGTCTTCATTCA
AGTGCTTTGCAGTTACAGTACACTGCCTCTTTACGCCATCGTCTCACAGGTAACAAGCTA
ATTTTTACTAAAAAGCTTGAGAATCTTTTGGTTTGGTTTTTTGGATTCTTTCTGAAAAGT
TGA
Protein Translation:
MGHGGEGMSLEFTPTWVVAGVCTVIVAISLAVERLLHYFGTVLKKKKQKPLYEALQKVKE
GTVHTHTHQQLMLLGFISLLLTVFQGLISKFCVKENVLMHMLPCSLDSRREAGASEHKNV
TAKEHFQTFLPIVGTTRRLLAEHAAVQVGYCSEKGKVPLLSLEALHHLHIFIFVLAISHV
TFCVLTVIFGSTRIHQWKKWEDSIADEKFDPETALRKRRVTHVHNHAFIKEHFLGIGKDS
VILGWTTHCKGNPKLNFHKYMMRALEDDFKQVVGISWYLWIFVVIFLLLNVNGWHTYFWI
AFIPFALLLAVGTKLEHVIAQLAHEVAEKHVAIEGDLVVKPSDEHFWFSKPQIVLYLIHF
ILFQNAFEIAFFFWIWGLHSSALQLQYTASLRHRLTGNKLIFTKKLENLLVWFFGFFLKS*
Alignment of T10P11.12.pep (top) and barley Mlo (Z83834, bottom). The two
sequences share 40% identity and 50% similarity.
. . . . .
1 ..MGHGGEGMSLEFTPTWVVAGVCTVIVAISLAVERLLHYFGTVLKKKKQ 48
| | ||.| || | .| :|. .| || | . : .
1 MSDKKGVPARELPETPSWAVAVVFAAMVLVSVLMEHGLHKLGHWFQHRHK 50
. . . . .
49 KPLYEALQKVKEGTVHTHTHQQLMLLGFISLLLTVFQG.LISKFCVKENV 97
| |:|||:|.| :|||.||||||| | | :|.| |: |.
51 KALWEALEKMK.........AELMLVGFISLLLIVTQDPIIAKICISEDA 91
. . . . .
98 LMHMLPCSLDSRREAGASEHKNVTAKEHFQTFLPIVGTTRRLLAEHAAVQ 147
| || || | ..:
92 ADVMWPCKR...........................GTEGRKPSKY.... 110
. . . . .
148 VGYCSEKGKVPLLSLEALHHLHIFIFVLAISHVTFCVLTVIFGSTRIHQW 197
| || | ||| |:| .|| ||:||||||: |||: |:|: :. |
111 VDYCPE.GKVALMSTGSLHQLHVFIFVLAVFHVTYSVITIALSRLKMRTW 159
. . . . .
198 KKWEDSIADEKFDPETALRKRRVTH....VHNH.............AFIK 230
|||| .: : | || | | || :
160 KKWETETTSLEYQFANDPARFRFTHQTSFVKRHLGLSSTPGIRWVVAFFR 209
. . . . .
231 EHFLGIGKDSVI...LGW.TTHCKGNPKLNFHKYMMRALEDDFKQVVGIS 276
: | : | : |. | | | .||||. |.:||||| |||||
210 QFFRSVTKVDYLTLRAGFINAHLSQNSKFDFHKYIKRSMEDDFKVVVGIS 259
. . . . .
277 WYLWIFVVIFLLLNVNGWHTYFWIAFIPFALLLAVGTKLEHVIAQLAHEV 326
|| :: | |.:|| | ||.||| :|| |||||| :| ::| |:
260 LPLWGVAILTLFLDINGVGTLIWISFIPLVILLCVGTKLEMIIMEMALEI 309
. . . . .
327 AEKHVAIEGDLVVKPSDEHFWFSKPQIVLYLIHFILFQNAFEIAFFFWIW 376
:: |.| ||.||.. ||| :| ||: || ||||||:.| | |
310 QDRASVIKGAPVVEPSNKFFWFHRPDWVLFFIHLTLFQNAFQMAHFVWTV 359
. . . . .
377 GLH..SSALQLQYTASLRHRLTGNKLIFTKKLENLLVWFFGFFLKS*... 421
| |: . | | | .: : |
360 ATPGLKKCYHTQIGLSIMKVVVGLALQFLCSYMTFPLYALVTQMGSNMKR 409
created 11 Aug 97
Larry Parnell