Gene T13L16.11
Putative Identification glycine-rich, zinc-finger DNA-binding protein
Position 59166 to 60258, from the initial methionine into the 3'-UTR
Strand +
EST match T76328
Database match glycine-rich DNA-binding proteins of Arabidopsis, tobacco, Xenopus, and S. pombe.

 

mRNA sequence:  Based a comparison to EST T76328, the 3'-UTR extends from position 60066 to 60258.

 

CDS:  The table below lists the coordinates of the single T13L16.11 exon and which exon predicting algorithms selected the given termini (Fe = FEXA, Fg = FGENEA, GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene - selects splice sites only, not exons).

Exon

Range

5'

3'

1 59166 - 60065 GS,Gr Fe,Fg,GS,Gr

Complete CDS of T13L16.11

ATGGAAGATCAATCGGCGGCGAGATCTATCGGAAAAGTTAGTTGGTTTAGCGATGGAAAA
GGCTACGGTTTCATTACTCCCGATGATGGCGGCGAAGAGCTTTTTGTTCATCAATCCTCA
ATTGTCTCCGATGGATTCCGTAGCTTGACTCTTGGTGAATCGGTTGAATACGAAATCGCG
CTTGGAAGCGACGGAAAGACTAAGGCCATTGAAGTTACTGCTCCCGGCGGCGGTTCACTT
AATAAGAAGGAAAACAGCTCCCGTGGATCCGGAGGTAATTGTTTTAATTGCGGTGAGGTT
GGTCATATGGCTAAGGATTGTGACGGTGGAAGTGGTGGGAAAAGCTTTGGAGGTGGTGGT
GGTCGTAGATCTGGCGGCGAAGGTGAATGTTACATGTGTGGTGATGTGGGACACTTCGCT
AGGGATTGTAGGCAAAGCGGTGGTGGAAACAGCGGCGGAGGAGGAGGAGGTGGTCGTCCG
TGTTACAGTTGCGGTGAGGTTGGTCATTTGGCAAAGGATTGTCGTGGTGGTTCTGGTGGA
AACAGATACGGAGGAGGTGGTGGTCGTGGATCTGGCGGTGATGGATGCTACATGTGTGGT
GGTGTGGGACATTTCGCTAGGGATTGTAGGCAAAACGGCGGTGGAAACGTTGGAGGTGGT
GGAAGCACTTGTTATACCTGTGGCGGAGTTGGCCACATTGCGAAAGTTTGCACCAGCAAG
ATACCTTCTGGTGGTGGTGGTGGTGGTCGTGCTTGTTACGAATGTGGAGGAACTGGCCAT
TTGGCTCGTGACTGTGATCGGAGAGGAAGTGGAAGCAGCGGAGGTGGCGGTGGCAGTAAC
AAGTGCTTCATCTGCGGCAAGGAAGGTCACTTTGCGAGGGAATGTACTTCGGTTGCTTAA

 

Protein sequence of T13L16.11

MEDQSAARSIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSDGFRSLTLGESVEYEIA
LGSDGKTKAIEVTAPGGGSLNKKENSSRGSGGNCFNCGEVGHMAKDCDGGSGGKSFGGGG
GRRSGGEGECYMCGDVGHFARDCRQSGGGNSGGGGGGGRPCYSCGEVGHLAKDCRGGSGG
NRYGGGGGRGSGGDGCYMCGGVGHFARDCRQNGGGNVGGGGSTCYTCGGVGHIAKVCTSK
IPSGGGGGGRACYECGGTGHLARDCDRRGSGSSGGGGGSNKCFICGKEGHFARECTSVA*

 

Alignment of glycine-rich proteins of Arabidopsis, tobacco, Xenopus, and S. pombe. The plant glycine-rich protein 2 (GRP2) sequences are described as components of the cell wall. The frog and yeast sequences contain DNA-binding zinc-finger motifs. A signature for cold-shock proteins is found in the plant sequences as are CCHC zinc-finger motifs.

           1                                                         60
   AtGRP2  MSGDNGGGERRKGSVKWFDTQKGFGFITPDDGGDDLFVHQSSIRSEGFRSLAAEEAVEFE 
  TobGRP2  ~~MAEESGQRAKGTVKWFSDQKGFGFITPDDGGEDLFVHQSGIRSEGFRSLAEGETVEFE 
T13L16.11  ~~MEDQSAARSIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSDGFRSLTLGESVEYE 
   XlCNBP  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
   SpBYR3  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 

           61                                                       120
   AtGRP2  VEIDNNNRPKAIDVSGPDGAPV..QGNSGGGSSG...GRGGFGGGR....GGGRGSGGGY 
  TobGRP2  VESGGDGRTKAVDVTGPDGAAV..QGGRGGGGGGGGRGGGGYGGGSGGYGGGGRGGSRGY 
T13L16.11  IALGSDGKTKAIEVTAPGGGSLNKKENSSRGSGGNCFNCGEVGHMAKDCDGG..SGGKSF 
   XlCNBP  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
   SpBYR3  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 

          121                                                      180
  AtGRP2  GGGGGGYGGRGGGGRGGSDCYKCGEPGHMARDCSEGGGGYGGGGG.....GY..GGGGGY 
 TobGRP2  GGGDGGYG..GGGGYGGGSRYGGGGGGY.GGGGGYGGGGSGGGSG.....CFKCGESGHF 
T13L16.11  GGG....GGRRSGGEG..ECYMCGDVGHFARDCRQSGGGNSGGGGGGGRPCYSCGEVGHL 
  XlCNBP  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSSNECFKCGRTGHW 
  SpBYR3  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MESESVPTVPQTTRPGPRCYNCGENGHQ 

           181                                                      240
   AtGRP2  .......GGG..GGGYGGGGRGGGGGGGSCYSCGESGHFARDCTSGGR*~~~~~~~~~~~ 
  TobGRP2  ARDCSQSGGG..GGGGRFGGGGGGGGGGGCYKCGEDGHFARECTSGGR*~~~~~~~~~~~ 
T13L16.11  AKDC..RGGS..GGNRYGGGGGRGSGGDGCYMCGGVGHFARDCRQNGGGNVGGGGSTCYT 
   XlCNBP  ARECPTGGGRGRGGGRGRGRGGFSSSRDICYRCGESGHLAKDC........DLQEDACYN 
   SpBYR3  ARECTKG....................SICYNCNQTGHKASECTE......PQQEKTCYA 

           241                                                      300
   AtGRP2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  TobGRP2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
T13L16.11  CGGVGHIAKVCTSKIPSGGGGGGRACYECGGTGHLARDCDRRGSGSSGGGGG..SN.KCF 
   XlCNBP  CGRGGHIAKDC..KEPRKER..EQCCYNCGKPGHLARDC............DHADEHRCY
   SpBYR3  CGTAGHLVRDCP.SSPNPRQ..GAECYKCGRVGHIARDCRTNGQQSGGRFGGHRSNMNCY

           301                                                      360
   AtGRP2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  TobGRP2  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
T13L16.11  ICGKEGHFARECTSVA*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
   XlCNBP  SCGEFGHIQKDCTK.VKCYRCGETGHVAINCSKTSEVN.CYRCGESGHLARECTIEATA*~
   SpBYR3  ACGSYGHQARDCTMGVKCYSCGKIGHRSFECQQASDGQLCYKCNQPGHIAVNCTSPVIEA*

 

Protein

cold-shock signature

CCHC zinc-finger signature

AtGRP2

yes

2 copies

TobGRP2

yes

2 copies

T13L16.11

yes

7 copies

XlCNBP

no

6 copies

SpBYR3

no

6 copies

 

written 23 Nov 97
Larry Parnell