CDS:  The table below lists the coordinates of the single T13L16.12 exon and which exon predicting algorithms selected the given termini (Fe = FEXA, Fg = FGENEA, GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene - selects splice sites only, not exons).

Complete CDS of T13L16.12

ATGGATTTGGCTAAAGTCTCTTGCTTTCTCTTAGCGATTATGTTTTTCATCAATGGTGGT
TCCTCCACCACGTTCACCATCGTTAACCAGTGTAACTACACCGTCTGGCCGGGACTTCTC
TCCGGCGCCGGCACCGCTCCATTACCCACCACTGGTTTCTCCCTCAACTCCTTTGAGTCA
CGACTCATCTCCATACCCGCCGCTTGGTCCGGCCGCATATGGGGACGCACGCTCTGCAAC
CAAAACGAAATCACCGGAATATTCACTTGCGTCACCGGCGACTGCGGCTCATCCCAAATC
GAATGCTCTGGCGCAGGAGCAATCCCTCCAGCTACACTAGCCGAATTCACACTCAACGGC
GCCGAAAATCTAGATTTCTACGACGTTAGCCTCGTCGATGGTTACAACATCCCAATGACG
ATCGTTCCTTACGGCGGAGCTATCGGAGTCGGTAAGTGTAACGCAACGGGTTGCGCGGCG
GATCTCAACGGCTTATGTCCGGAGCAGCTGAAAGTAACGGTTGAAGCGGCTGGAACGGGT
GCTGTGGCGTGCAAGAGCGCTTGTGAGGCGTTTGGAACGCCGGAGTTTTGTTGTAACGGC
GCGTTTGGAACGCCGGACACGTGTCAGCCGAGTGAGTACTCTGTTTTCTTTAAGAAAACT
TGTCCGACGGCTTATAGTTACGCTTATGACGACGGCACAAGCACTTTTACTTGCTCCGGT
GCTGATTACGTCATCACTTTCTGTCCTTCTCCTTCAAGGTAA

Protein sequence of T13L16.12

MDLAKVSCFLLAIMFFINGGSSTTFTIVNQCNYTVWPGLLSGAGTAPLPTTGFSLNSFES
RLISIPAAWSGRIWGRTLCNQNEITGIFTCVTGDCGSSQIECSGAGAIPPATLAEFTLNG
AENLDFYDVSLVDGYNIPMTIVPYGGAIGVGKCNATGCAADLNGLCPEQLKVTVEAAGTG
AVACKSACEAFGTPEFCCNGAFGTPDTCQPSEYSVFFKKTCPTAYSYAYDDGTSTFTCSG
ADYVITFCPSPSR*

Alignment of selected pathogenesis-related proteins. There is significant sequence conservation between members of the pathogenesis-related protein family. The alignment below demonstrates this for zeamatin from Z. mays, permatin from oat, Arabidopsis thaumatin-like proteins, and C. elegans protein F28D1.4.

             1                                                         60
 ZmZeamatin  ~~~~~~~~~~MAGSVAIVGIFVALLAV...AGEAAVFTVVNQCPFTVWAASVPVGG.... 
OatPermatin  ~~~~~~~~~~MASSSVLQLLPLLMLAITIATTDAATITVVNKCSYTVWPGALP.GG.... 
  T13L16.12  ~~~~~~~~~~~~MDLAKVSCFLLAIMFFINGGSSTTFTIVNQCNYTVWPGLLSGAGTAPL 
  AtT19F6.2  MIITVLHSHVSFYFIILSFLFFHAKALHLVGSDGATITIVNRCSFTVWPGILSNSGSGDI 
      AtLP3  ~~~~~~~~~~~MAKISSIHILFFVFITSGIADSATVFTLQNSCAYTVWPGTLSG.NSITL 
  CeF28D1.4  ~~~~~~~~~~~~~~~MAFIKLTLAVILMSLGAESRTITILNKCKFTIWPGIL...GPGNP 

             61                                                       120
 ZmZeamatin  ...GRQLNRGESWRITAPAGTTAARIWARTGCKFDA.SGRGSCRTGDCG.GVLQCTGYGR 
OatPermatin  ...GVRLDPGKSWALNMPAGTRGARVWPRTGCTFDA.SGRGHCVTGDCG.GALACRVSGQ 
  T13L16.12  PTTGFSLNSFESRLISIPAAW.SGRIWGRTLCNQNEITGIFTCVTGDCGSSQIECSGAGA 
  AtT19F6.2  GTTGFELVPGGSRSFQAPASW.SGRFWARTGCNFNSDTGQGTCLTGDCGSNQVECNGAGA 
      AtLP3  GDGGFPLTPGASVQLTAPTGW.SGRFWARTGCNFDA.SGHGTCVTGDCG.GVLKCTG.GG 
  CeF28D1.4  AEGGFKLNAGEPRDIDVDDGWTAGRIWARTGCNEN.....FDCDTGSCGVKLRACNGAGG 

             121                                                      180
 ZmZeamatin  .APNTLAEYALKQFNN.....LDFFDISLIDGFNVPMSFLPDGGS...GCSRGPRCAVDV 
OatPermatin  .QPATLAEYTLGKGGA.....KDFFDLSVIDGFNVPMSFQPVGGA...AC.RGATCAADI 
  T13L16.12  IPPATLAEFTLNG.....AENLDFYDVSLVDGYNIPMTIVPYGGAIGVGKCNATGCAADL 
  AtT19F6.2  KPPATLAEFTIGSGPADPARKQDFYDVSLVDGYNVPMLVEASGGS..EGTCLTTGCVTDL 
      AtLP3  VPPATLAEFTVGSSNAG....MDFYDVSLVDGYNVKMGIKPQGG...FGNCKYAGCVSDI 
  CeF28D1.4  VPPASLAEFTLNGDGG.....KDFYDVSLVDGYNLPVLIDPNDGS...GCDRAGGCVKDM 

             181                                                      240
 ZmZeamatin  NARCPAELRQDG........VCNNACPVFKKDEYCCVGSAA..NDCHPTNYSRYFKGQCP 
OatPermatin  TKQCPQELKVAG........GCASACGKFGGDTYCCRGQFT..DKCPPTNYSKFFKGKCP 
  T13L16.12  NGLCPEQLKVTVEAAGTGAVACKSACEAFGTPEFCCNGAFGTPDTCQPSEYSVFFKKTCP 
  AtT19F6.2  NQKCPTELRF.....GSGS.ACKSACEAFGSPEYCCSGAYASPTECKPSMYSEIFKSACP 
      AtLP3  NEICPSELRIMDPNSGSVA.ACKSAC.AFSSPEFCCTGAHATPQTCSPTYYSSMFKNACP 
  CeF28D1.4  NAECPADLAVQGSNGNT..VACKFGCVGYNTDEECCRGVRNTPETCDRSTTAQKFKDACP 

             241                          272
 ZmZeamatin  DAYSYPKDDATSTFTCPAGTNYKVVFCP*~~~~
OatPermatin  DAYSYAKDDQTSTFTCPVGTNYQIVLCP*~~~~
  T13L16.12  TAYSYAYDDGTSTFTC.SGADYVITFCPSPSR*
  AtT19F6.2  RSYSYAFDDATSTFTC.TDADYTITFCPSLPR*
      AtLP3  SAYSYAYDDASSTFTC.TGSNYLITFCPTQS*~
  CeF28D1.4  TAYSYAYDDKSSTFTCQTGATYTVQFC*~~~~~

written 23 Nov 97
Larry Parnell