Gene T13L16.13
Putative Identification senescence-associated rhodanese-like protein
Position 63632 - 64539, from the initial methionine to the termination codon
Strand +
EST match none, but T44949 is similar
Database match senescence-associated proteins of Arabidopsis and R. sativus

 

CDS:  The table below lists the coordinates of the exons for T13L16.13 and which exon predicting algorithms selected the given termini (Fe = FEXA, Fg = FGENEA, GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene - selects splice sites only, not exons). Splice sites suggested by EST T44949 are indicated by est.

Exon

Range

5'

3'

1 63632 - 63714 Fg,Gr est,GS,NPG
2 63811 - 63961 est,M,NPG est,FG,GS,Gr,M,NPG
3 64080 - 64133 est,GS,NPG GS,NPG
4 64232 - 64320 Fe,Fg,GS,Gr,M,NPG Fg,Gr,M,NPG
5 64464 - 64539 NPG  

Complete CDS of T13L16.13

ATGTCAGAACCAAAAGTCATTACCATCGATGTAAACCAAGCTCAGAAACTTCTTGATTCT
GGCTACACGTTTCTTGATGTCAGAACTGTTGAAGAGTTCAAGAAAGGCCATGTTGATTCA
GAAAATGTCTTCAATGTTCCTTACTGGTTATATACTCCACAAGGTCAAGAGATTAACCCT
AATTTCTTGAAACATGTTTCATCTCTCTGTAACCAAACCGATCATCTCATCCTGGGATGT
AAATCTGGAGTTAGATCATTACATGCTACCAAATTTCTTGTTTCTTCCGGCTTCAAGACT
GTAAGAAACATGGACGGTGGTTACATTGCTTGGGTCAACAAGAGATTCCCAGTTAAAGTG
GAACACAAGGAACTCAAAGAGATGAAAAAATGGGCAGACCGACCAATGAAGCTCCAGCAT
CGACAATGTATGTATTCCCGGAGATATATTTAG

 

Protein sequence of T13L16.13

MSEPKVITIDVNQAQKLLDSGYTFLDVRTVEEFKKGHVDSENVFNVPYWLYTPQGQEINP
NFLKHVSSLCNQTDHLILGCKSGVRSLHATKFLVSSGFKTVRNMDGGYIAWVNKRFPVKV
EHKELKEMKKWADRPMKLQHRQCMYSRRYI*

 

Multiple sequence analysis of senescence-associated proteins. The R. sativus dark-inducible, senescence-associated DIN1 gene encodes a chloroplast protein (RsatDIN1). The Arabidopsis AtSAP gene also encodes a senescence-associated protein and is highly regulated during leaf senescence. The Arabidopsis AtKetoRes gene was cloned by complementation of a yeast erg3 sterol mutant and subsequent selection on ketoconazole. Rhodanese proteins catalyze the conversion of thiosulfate and cyanide to sulfite and thiocyanate. Residues shared by T13L16.13 and bovine rhodanese thiosulfate sulfurtransferase, (BovTHTR, EC 2.8.1.1) are highlighted. The structure for bovine mitochondrial thiosulfate sulfurtransferase has been solved. The enzyme consists of two domains that share little sequence similarity but have the same structure. The second of these domains, from residues 159 to 296 [SLLKT..KGGKA], contains the active site.

           1                                                         60
  BovTHTR  VHQVLYRALVSTKWLAESVRAGKVGPGLRVLDASWYSPGTREARKEYLERHVPGASFFDI

           61                                                       120
 RsatDIN1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MESALNTTARIRSWSSVISPPLQV
    AtSAP  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~METTAFNTTSRIGNWSSAISPPLQT
AtKetoRes  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
T13L16.13  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  BovTHTR  EECRDKASPYEVMLPSEAGFADYVGSLGISNDTHVVVYDGDDLGSFYAPRVWWMFRVFGH

           121                                                      180
 RsatDIN1  CESFKWKLPKATRRVVSVADRQNSNFRWRK.VTTGRANVAAEAARVPTSVPVRVARELAQ
    AtSAP  CGSFKCQLP..TRRGVIVADLRNSNFRWRKATTTSRGNVAAEAVKIPTSVPVRVARELAQ 
AtKetoRes  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MADESRVPSSVSVTVAHDLLL 
T13L16.13  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MSEPKVIT.IDVNQAQKLLD 
  BovTHTR  RTVSVLNGGFRNWLKEGHPVTSEPSRPEPAIFKATLNRSLLKTEYQVLENLESKRFQLVD

           181                                                      240
 RsatDIN1  AGYKYLDVRTPDEFSIGH.PCSAI....NVPYMYR.VGSGMVKNPSFLRQV..SSHFRKH 
    AtSAP  AGYRYLDVRTPDEFSIGH.PTRAI....NVPYMYR.VGSGMVKNPSFLRQV..SSHFRKH 
AtKetoRes  AGHRYLDVRTPEEFSQGHVPVGSI....NVPYMNR.GASGMSKNPDFLEQV..SSHFGQS 
T13L16.13  SGYTFLDVRTVEEFKKGHVDSENV...FNVPYWLY.TPQGQEINPNFLKHV..SSLCNQT 
  BovTHTR  SRAQGRYLGTQPEPDAVGLDSGHIRGSVNMPFMNFLTEDGFEKSPEELRAMFEAKKVDLT

           241                                                      300
 RsatDIN1  DEIIIGCESGERSLMASTELLTAGFTGVTDIAGGYVAWTENELPVEE*~~~~~~~~~~~~ 
    AtSAP  DEIIIGCESGQMSFMASTDLLTAGFTAITDIAGGYVAWTENELPVEE*~~~~~~~~~~~~ 
AtKetoRes  DNIIVGCQSGGRSIKATTDLLHAGFTGVKDIVGGYSAWAKNGFLQKLDDSPFTKHLSLSL 
T13L16.13  DHLILGCKSGVRSLHATKFLVSSGFKTVRNMDGGYIAWVNKRFPVKVEHKELKEMKKWAD 
  BovTHTR  KPLIATCRKGVTACHIALAAYLCGKPDVAIYDGSWFEWFHRAPPETWVSQGKGGKA*~~~

           301           317
 RsatDIN1  ~~~~~~~~~~~~~~~~~~
    AtSAP  ~~~~~~~~~~~~~~~~~~
AtKetoRes  RLLLLQ*~~~~~~~~~~~
T13L16.13  RPMKLQHRQCMYSRRYI*
  BovTHTR  ~~~~~~~~~~~~~~~~~~

 

written 24 Nov 97
Larry Parnell