Gene T13L16.14
Putative Identification physical impedence protein of uncharacterized function
Position 66543 - 68418, from the initial methionine to the termination codon
Strand +
EST match T43413
Database match Z. mays physical impedence protein IIG2

 

CDS:  The table below lists the coordinates of the exons for T13L16.14 and which exon predicting algorithms selected the given termini (Fe = FEXA, Fg = FGENEA, GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene - selects splice sites only, not exons). Splice junctions delineated by identity to EST T43413 are designated by EST.

Exon

Range

5'

3'

1 66543 - 67471 Fe, Fg, Gr EST, GS, Gr, M, NPG
2 67805 - 67971 EST, GS, Gr, M, NPG Gr, GS, NPG
3 68059 - 68143 Fe, GS, Gr, M Fe, GS, Gr, M, NPG
4 68241 - 68418 Gr, NPG GS, Gr

Complete CDS of T13L16.14

ATGGAATCCTCCGGCGACAAGCAAACTTCTTCTCTTTACCCTACTGTTGACACATCCAAT
CCCGAAGCTCCTATAAACCCTAGCTCATCATCTTCTACTAACAATCTCTATCCTTCTCTC
GATATGAATGATCTCGCTCGTAATCTCTTCCCTGAACAACCAGAGACGAGTTCTATTCCG
GTTTCAGCTCCTCCCGCTGCGACGGAGGAAGTAATTTTGAAAATCTCCGGCGCGATTCTT
CATTTAATCGACAAATCTTACAGCGTCGAGCTCGCTTGCGGTGATCTTGAGATCATCCGT
ATTGTTCAAGGAGAGAATGTTGTCGCTGTTCTCGCTAGTGTCTCCGATGAGATTCAATGG
CCGTTGACTAAGGATGAGAACTCTGTCAAGGTCGATGAGTCTCATTACTTCTTCACGCTT
CGTCCTACCAAAGAGATCTCACATGATTCAAGCGACGAAGAAGACGGAGATGGAGGGAAA
AACACTAACGAGATGTTGAACTACGGACTTACAATTGCTTCTAAAGGTCAAGAGCATTTA
CTGGTGGAGCTTGAGAAGATCTTGGAGGATTATAGTAGTTTCTCTGTGCAGGAGGTGTCA
GAGGAAGCGAAGGAAGCAGGTGAAAAGGTTTTGGATGTGACGGTGGCGAGAGAGACGTCT
CCGGTGGAGCTTACTGGGGAAAGGAAAGAGATTGTGGAGAGGCAATGTTCTGCTTATTGG
ACTACTTTGGCTCCGAATGTGGAGGATTATAGCGGGAAGGCGGCGAAGCTGATTGCAACT
GGCTCAGGACATTTGATAAAAGGGATTCTTTGGTGTGGAGATGTGACTATGGATAGGCTT
ATTTGGGGAAATGGTTTCATGAAAAGGAGGTTGTCAAAGGCTGAGAAGGAGAGTGAAGTT
CACCCTGACACCTTGAAAAGAATCAGAAGAGTGAAGAGGATGACCAAAATGACAGAGAGT
GTAGCAAACAGCATACTGTCTGGAGTCCTTAAAGTTTCTGGATTCTTCACAAGTTCAGTG
GCAAACACCAAAGTAGGAAAGAAATTCTTCAGCCTTCTTCCTGGAGAAGTCATCCTTGCA
TCTCTTGACGGATTCAATAAGGTCTGTGACGCTGTTGAAGTAGCTGGGAGGAATGTAATG
TCAACCTCTTCCACTGTTACAACCGAACTCGTTGATCACAAGTATGGTGGTAAAGCGGCT
GAGGCGACAAACGAAGGGCTCGATGCAGCAGGATATGCGCTTGGCACAGCCTGGGTTGCT
TTCAAAATAAGAAAGGCTATTAACCCGAAGAGTGTACTCAAGCCTTCGACGCTTGCAAAA
ACCGCCATTAGATCTGCAGCTTCACAAAAAAAAGCCTAG

 

Protein sequence of T13L16.14

MESSGDKQTSSLYPTVDTSNPEAPINPSSSSSTNNLYPSLDMNDLARNLFPEQPETSSIP
VSAPPAATEEVILKISGAILHLIDKSYSVELACGDLEIIRIVQGENVVAVLASVSDEIQW
PLTKDENSVKVDESHYFFTLRPTKEISHDSSDEEDGDGGKNTNEMLNYGLTIASKGQEHL
LVELEKILEDYSSFSVQEVSEEAKEAGEKVLDVTVARETSPVELTGERKEIVERQCSAYW
TTLAPNVEDYSGKAAKLIATGSGHLIKGILWCGDVTMDRLIWGNGFMKRRLSKAEKESEV
HPDTLKRIRRVKRMTKMTESVANSILSGVLKVSGFFTSSVANTKVGKKFFSLLPGEVILA
SLDGFNKVCDAVEVAGRNVMSTSSTVTTELVDHKYGGKAAEATNEGLDAAGYALGTAWVA
FKIRKAINPKSVLKPSTLAKTAIRSAASQKKA*

 

Comparison of T13L16.14 (top) to Z. mays IIG2 physical impedence protein (bottom). Admittedly, it is possible that the initial methionine begins at position 67401 and that the resulting peptide begins with MKRRLSK. However, because the name of the maize protein is based upon a process and not its biochemical role and because incorporation of purely hypothetical alternate exons does not extend the similarity shown, the model of gene T13L16.14 is as indicated above.

                  .         .         .         .         .
     251 SGKAAKLIATGSGHLIKGILWCGDVTMDRLIWGNGFMKRRLSKAEKESEV 300
                                                 :   :  .||
       1 ........................................MQPGDANAEV 10
                  .         .         .         .         .
     301 HPDTLKRIRRVKRMTKMTESVANSILSGVLKVSGFFTSSVANTKVGKKFF 350
          |: |:||:| ||.....| ||  |||||.||.|:||||.||.| |||||
      11 SPEMLRRIKRAKRVSQISEKVATGILSGVVKVTGYFTSSLANSKAGKKFF 60
                  .         .         .         .         .
     351 SLLPGE.VILASLDGFNKVCDAVEVAGRNVMSTSSTVTTELVDHKYGGKA 399
           .     :||||||| .        ||           :        : 
      61 QHVAWRGSVLASLDGFGEDLATPVEGGRKERFVHVVNCDDRASISQVRRQ 110
                  .         .         .         .         .
     400 AEATNEGLDAAGYALGTAWVAFKIRKAINPKSVLKPSTLAKTAIRSAASQ 449
         .   ||       |     |  ::.     .   :| |     :     .
     111 SRRRNERRAGRRRARHRDGVG.RVQDPAGLEPQERPQTHGAGHVHHQGQR 159

 

written 19 Dec 97
Larry Parnell