Gene T13L16.16
Putative Identification histidine kinase
Position 76379 - 81208, from the initial methionine to the termination codon
Strand +
EST match none
Database match CKI1, A. thaliana histidine kinase homolog (see note)

 

Note:  The histidine kinase domain of T13L16.16 resides approximately from amino acid 490 to 1190. The N-terminal 480 amino acids have no match to any database entry.

 

CDS:  The table below lists the coordinates of the exons for T13L16.16 and which exon predicting algorithms selected the given termini (Fe = FEXA, Fg = FGENEA, GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene - selects splice sites only, not exons).

Exon

Range

5'

3'

1 76379 to 76582 Gr Fe, Fg, GS, N
2 76751 to 76993 Fe, Fg, GS, Gr, M, N Fe, Fg, GS, Gr, M, N
3 77072 to 77845 Fg, GS, Gr, N Fg, GS, Gr, N
4 77932 to 78179 Fe, Fg, GS, Gr, M, N Fe, Fg, GS, N
5 78281 to 78440 GS, Gr, M GS, Gr, M, N
6 78646 to 78772 GS, Gr, N GS, Gr, N
7 78870 to 78959 Fe, GS, Gr, M, N Fe, GS, Gr, M, N
8 79044 to 79238 GS, Gr, M, N Gr, M
9 79339 to 79408 Gr, M, N GS, Gr, M, N
10 79503 to 79545 Fg, GS, M, N Fg, GS, M, N
11 79629 to 79766 Fg, GS, Gr, M, N Fg, GS, Gr, M, N
12 79858 to 80901 Fe, Fg, GS, Gr, M, N Fe, Fg, GS, M, N
13 80972 to 81208 Fg, GS, Gr, N Fg, GS, Gr

Complete CDS of T13L16.16:  If the translation start at position 76328 is chosen (as predicted by Fe, Fg, and GS), the CDS contains the bracketed sequence

[ATGCGAGGAGATAGCTTCTCAATGAGTATTGAGAATCTTCCTGATTCTCCA]
ATGGGTTCGAGGAAGAAGAAGATGCAGATCAGGAAGGTGTTCGATAAAATGACTGAATGG
GTTACCCCTTGGAGAAGTAATTTGGAGAGTCCAAGAGAGATGATGATTTTGCGTGGAGAT
GTTGAACAAGATGAGTTTCAATACGCTAGTAGTCACTGTTTGTCTTCTTACTACAGTGTC
TTTGTTGTTCGCCTCGCTATAATGGTTATGTTAGCAATTTTGATAGGGCTTTTAACGGTT
CTAACATGGCATTTCACAAGGATTTATACAAAGCAGTCGCTTCAAACATTAGCGTATGGC
CTTAGATACGAGCTTCTTCAGCGTCCAGTCTTACGGATGTGGAGCGTTTTAAACACCACA
TCAGAGCTAACAACAGCTCAAGTCAAGTTATCTGAATATGTCATCAAAAAATATGACAAG
CCAACAACTCAAGAAGAACTTGTGGAGATGTATCAAGCAATGAAGGATGTGACATGGGCT
CTGTTTGCGAGTGCCAAAGCTCTAAATGCCATAACCATAAACTACAGAAATGGATTTGTC
CAAGCTTTTCACAGAGATCCAGCAAGTAGCAGCACATTCTACATCTTCTCCGATCTAAAA
AACTATTCCATCAGTGGAACAGGGCCTGAAGATGTAAGTGGGTGGAACAACAAGTCAATA
CACGGGAACATGTCTGCCATTTGGTATCAGCAACAGCTTGATCCAGTAACAGGGGAAAAT
TTGGGAAAGCCTCTTAAAATTCCACCGGATGATCTGATCAACATCGCAGGAATCTCACAA
GTGCCTGATGGTGAAGCTTCTTGGCATGTGACAGTGAGCAAATACATGGACTCTCCGCTT
CTCTCAGCGGCTTTGCCTGTCTTTGATGCATCAAACAAGAGTATTGTGGCTGTTGTGGGG
GTGACAACTGCACTTTACAGCGTAGGACAGTTGATGAGAGATCTTGTAGAAGTCCACGGT
GGACATATTTATTTGACATCTCAAGAAGGATACTTGCTTGCTACCTCCACAGATGGTCCT
CTTTTGAAAAATACATCAAACGGGCCTCAGCTAATGAAAGCCACTGATTCAGAAGAGTGG
GTTATAAAGTCAGGAGCTCAGTGGTTAGAGAAAACTTATGGAAGTAAGCGCCCTCACGTT
GTTCATGCTGAGAATGTAAAGCTTGGTGACCAACGATATTACATTGATTCATTCTATCTT
AATCTCAAGAGACTTCCAATTGTAGGTGTTGTCATTATTCCCAGGAAGTTCATTATGGGA
AAAGTGGATGAAAGGGCCTTCAAGACTTTGATTATTCTGATTTCTGCTTCTGTCTGCATC
TTCTTTATTGGATGTGTCTGCATTTTAATACTCACAAATGGAGTTTCAAAGGAGATGAAG
CTAAGAGCAGAACTGATAAGGCAACTGGATGCAAGAAGAAGAGCTGAAGCTTCAAGCAAC
TACAAAAGCCAGTTTTTGGCAAATATGAGTCATGAGTTGAGGACACCAATGGCTGCTGTA
ATTGGATTACTAGATATTCTTATATCGGATGATTGTCTTTCAAATGAGCAATACGCAACA
GTCACTCAGATCAGAAAGTGCTCTACAGCTCTCCTTCGGCTTCTCAATAATATACTGGAC
TTGAGCAAGGTTGAGTCAGGAAAGCTTGTTCTGGAAGAAGCTGAGTTTGATTTGGGGCGA
GAACTTGAAGGACTTGTTGACATGTTCTCGGTGCAGTGTATTAATCACAATGTAGAGACA
GTTCTAGACCTCTCTGATGATATGCCAGCATTAGTCAGAGGAGACTCAGCAAGACTTGTT
CAAATATTTGCAAATCTTATAAGCAATTCTATAAAGTTTACAACAACGGGTCACATTATT
CTTCGTGGATGGTGCGAGAACATAAATTCTCTACATGATGAGATGAGCGTATCTGTTGAC
AGAAGGAAACCATGGGCTCCAATGAAGACAAAACAGGTGCAACACAGAAATCACTTGCAG
AAGTCTTGTAAGAACGCGAACAAAATGGTTCTTTGGTTTGAGGTTGATGATACTGGATGT
GGAATAGATCCAAGCAAATGGGACTCTGTTTTTGAGAGCTTTGAGCAAGCTGATCCTTCT
ACCACTCGGACGCACGGAGGAACTGGACTTGGACTATGTATTGTGCGAAACTTGGTTAAC
AAAATGGGTGGAGAAATCAAAGTAGTGCAGAAGAATGGCTTAGGGACTCTTATGAGACTG
TACTTGATTTTGAGTACTCCTGACACTGTAGATCAAAACATCCAGCCAGATTTCTCCAAA
TACGGTCTCGTGGTTATGCTTTCCATGTATGGAAGTACAGCAAGAATGATCACATCAAAG
TGGTTGCGAAAACACGGCATTGCAACTGTTGAAGCATCAGACTGGAACGAGCTGACTCAG
ATCATTAGAGACCTCTTGGAGACGGGAAGCCGTGACAACAGCTTTGATTCACAGCACAAT
ATATCTGATCCACTGAGAGCAGAGCTTTCAAATATAGTAGAAATCAAGAACCCTGTGTTT
GTCATAGTGGTGGATATCGGAGTACTTGATCTCACCACAAATATATGGAAGGAACAGCTT
AACTACCTCGACAGATTCTCCAACAAAGCAAAGTTTGCTTGGCTACTGAAGCATGACACC
TCCAATACAGTTAAAACAGAACTTAGACGAAAAGGGCATGTCATGATGGTTAACAAACCG
TTGTACAAGGCCAAAATGATCCAGATTCTGGAGGCTGTAATAAAAAACCGGAAGAGAGGC
TTGTGTAATGACTTAAGAAACAGAGGAAACGGAAGTGATGAATCCCACGATTGCTTAGAA
ATTGATCCAACACAATTCGACACTTGCAGTTCCGATGATTCCTCGGAGACATCTGGCGAG
AAACAAGTAGATAAATCTGTTAAACCAAGCACCTTGCACTCTCCAGTGCTTAAGAACTAT
CTCATCGATGCCACTACCAGCAATGATGACTCAACATCTGCAAGTATGACTCAAAAGAAC
CCAGAAGAAGAAGATTGGAAGGATCGGCTGTATTCAGGAATTGCGTTGGATGGAAAGAAT
CAGAAATCTCTTGAAGGTATAAGGATCTTGCTGGCAGAGGATACACCCGTTCTTCAACGA
GTAGCCACCATAATGCTTGAGAAAATGGGAGCAACTGTAACTGCAGTCTGGGATGGACAA
CAAGCAGTTGATTCCCTCAACTACAAGTCTATCAATGCTCAAGCTCCAACTGAAGAACAC
AAATCGTTCGAAGAAGAAACCGCTAACAAAGTTACAACTCGGGAGACTAGTTTACGGAAT
TCCTCGCCTTATGACTTGATCCTCATGGACTGCCAGATGCCAAAAATGGATGGATATGAG
GCAACAAAGGCGATAAGGAGAGCAGAGATTGGCACAGAGCTGCACATTCCAATCGTGGCG
TTGACAGCGCATGCAATGTCTTCTGATGAAGCGAAATGCTTGGAGGTTGGAATGGATGCT
TATCTCACAAAGCCTATTGATCGAAAGCTTATGGTCTCTACCATTTTGTCACTCACTAAA
CCATCAGCATTCCAAACTTCATTGTCCGCTTGA

 

Protein sequence of T13L16.16:  If the predicted translation start at 76328 is chosen, the leader peptide contains the bracketed sequence

[MRGDSFSMSIENLPDSP]
MGSRKKKMQIRKVFDKMTEWVTPWRSNLESPREMMILRGDVEQDEFQYASSHCLSSYYSV
FVVRLAIMVMLAILIGLLTVLTWHFTRIYTKQSLQTLAYGLRYELLQRPVLRMWSVLNTT
SELTTAQVKLSEYVIKKYDKPTTQEELVEMYQAMKDVTWALFASAKALNAITINYRNGFV
QAFHRDPASSSTFYIFSDLKNYSISGTGPEDVSGWNNKSIHGNMSAIWYQQQLDPVTGEN
LGKPLKIPPDDLINIAGISQVPDGEASWHVTVSKYMDSPLLSAALPVFDASNKSIVAVVG
VTTALYSVGQLMRDLVEVHGGHIYLTSQEGYLLATSTDGPLLKNTSNGPQLMKATDSEEW
VIKSGAQWLEKTYGSKRPHVVHAENVKLGDQRYYIDSFYLNLKRLPIVGVVIIPRKFIMG
KVDERAFKTLIILISASVCIFFIGCVCILILTNGVSKEMKLRAELIRQLDARRRAEASSN
YKSQFLANMSHELRTPMAAVIGLLDILISDDCLSNEQYATVTQIRKCSTALLRLLNNILD
LSKVESGKLVLEEAEFDLGRELEGLVDMFSVQCINHNVETVLDLSDDMPALVRGDSARLV
QIFANLISNSIKFTTTGHIILRGWCENINSLHDEMSVSVDRRKPWAPMKTKQVQHRNHLQ
KSCKNANKMVLWFEVDDTGCGIDPSKWDSVFESFEQADPSTTRTHGGTGLGLCIVRNLVN
KMGGEIKVVQKNGLGTLMRLYLILSTPDTVDQNIQPDFSKYGLVVMLSMYGSTARMITSK
WLRKHGIATVEASDWNELTQIIRDLLETGSRDNSFDSQHNISDPLRAELSNIVEIKNPVF
VIVVDIGVLDLTTNIWKEQLNYLDRFSNKAKFAWLLKHDTSNTVKTELRRKGHVMMVNKP
LYKAKMIQILEAVIKNRKRGLCNDLRNRGNGSDESHDCLEIDPTQFDTCSSDDSSETSGE
KQVDKSVKPSTLHSPVLKNYLIDATTSNDDSTSASMTQKNPEEEDWKDRLYSGIALDGKN
QKSLEGIRILLAEDTPVLQRVATIMLEKMGATVTAVWDGQQAVDSLNYKSINAQAPTEEH
KSFEEETANKVTTRETSLRNSSPYDLILMDCQMPKMDGYEATKAIRRAEIGTELHIPIVA
LTAHAMSSDEAKCLEVGMDAYLTKPIDRKLMVSTILSLTKPSAFQTSLSA*

 

Comparison of T13L16.16 and CKI1:  From the alignment present below it is clear that there are some regions of T13L16.16 and CKI1 that are highly conserved. 

                         .         .         .         .         .
  T13L16.16   1 MGSRKKKMQIRKVFDKMTEWVTPWRSNLESPREMMILRGDVEQDEFQYAS 50

                         .         .         .         .         .
  T13L16.16  51 SHCLSSYYSVFVVRLAIMVMLAILIGLLTVLTWHFTRIYTKQSLQTLAYG 100
                                      .:: .  ..      ::   .   . : 
     AtCKI1   1                       MMVKVTKLVASRPIVVFCVLAFLVVVFE 28
                         .         .         .         .         .
  T13L16.16 101 LRYELLQRPVLRMWSVLNTTSELTTAQVKLSEYVIKKYDKPTTQEELVEM 150
                  :    |      ...   .  |          |.   | |  .  .  
     AtCKI1  29 CIWISNWRTTTE..NLVKEVASFTEDLRTSLVSEIENIGKFTYAKTNLST 76
                         .         .         .         .         .
  T13L16.16 151 YQAMKDVTWALFASAKALNAITINYRNGFVQAFHRDPASSSTFYIFSDLK 200
                    : :   :  .      |          |:      |   ||  |  
     AtCKI1  77 IGLARVIDSYITNNDTGFTEIQTQIAPLLFVAYSTILQVSQVSYISRDGL 126
                         .         .         .         .         .
  T13L16.16 201 NYSISGTGPEDVSGWNNKSIHGNM.SAIWYQQQLDPVTGENLGKPLKIPP 249
                 :|        |. . | | . .     || | .| .||   |   |   
     AtCKI1 127 MFSYIAESNTSVAVFANSSSNSSRGDYTWYTQTVDQLTGRLNGNSTKSQS 176
                         .         .         .         .         .
  T13L16.16 250 DDLINIAGISQVPDGEASWHVTVSKYMDSPLLSAALPVFDASNKSIVAVV 299
                 |. .            .     .        .    |    .|    .|
     AtCKI1 177 LDVTHTDWFQAAQSNNYTTAFVGTSLGGEDNETLIQSVVSLYSKK..GLV 224
                         .         .         .         .         .
  T13L16.16 300 GVTTALYSVGQLMRDLVEVHGGHIYLTSQEGYLLATSTDGPLLKNTSNGP 349
                 .   . .. :.:  |  .||  :|: ..:| .|            ||| 
     AtCKI1 225 SLGFPVKTLTEVLNSL.NLHGEELYMWTKDGTVLVREGSLNDSFFISNGS 273
                         .         .         .         .         .
  T13L16.16 350 QLMKATDSEEWVIKSGAQWLEKTYGSKRPHVVHAENVKLGDQRYYIDSFY 399
                       .  |     .| : .   |          |.:   ||      
     AtCKI1 274 ICFGRESNSLW.....SQCIPENCSSS......GYEVEIKRLRYQAFCSV 312
                         .         .         .         .         .
  T13L16.16 400 LNLKRLPIVGVVIIPRKFIMGKVDERAFKTLIILISASVCIFFIGCV.CI 448
                : .  .|:   .. | |    ::  .| |    ||   : : |   |  :
     AtCKI1 313 IEVSGVPLRYTLMFPNKGGATRIKHQAEKAKYQLIVVMIFLGFGWPVWFV 362
                         .         .         .         .         .
  T13L16.16 449 LILTNGVSKEMKLRAELIRQLDARRRAEASSNYKSQFLANMSHELRTPMA 498
                  :     :|| :|| || |::| ..||  |  |||  || ||::|  :|
     AtCKI1 363 WFMMQATRREMHMRATLINQMEATQQAERKSMNKSQAFANASHDIRGALA 412
                         .         .         .         .         .
  T13L16.16 499 AVIGLLDILISDDCLSNEQYATVTQIRKCSTALLRLLNNILDLSKVESGK 548
                 . ||:||        .:   |. |:  |.  |. |||.:||:||:||||
     AtCKI1 413 GMKGLIDICRDGVKPGSDVDTTLNQVNVCAKDLVALLNSVLDMSKIESGK 462
                         .         .         .         .         .
  T13L16.16 549 LVLEEAEFDLGRELEGLVDMFSVQCINHNVETVLDLSDDMP...ALVRGD 595
                : | | :|.| : || .:| :    .   |: |||  |      . ||||
     AtCKI1 463 MQLVEEDFNLSKLLEDVIDFYHPVAMKKGVDVVLDPHDGSVFKFSNVRGD 512
                         .         .         .         .         .
  T13L16.16 596 SARLVQIFANLISNSIKFTTTGHIILRGWCENINSLHDEMSVSVDR.... 641
                | || ||  ||:||.:|||  ||| .| | :   |    .  |  :    
     AtCKI1 513 SGRLKQILNNLVSNAVKFTVDGHIAVRAWAQRPGSNSSVVLASYPKGVSK 562
                         .         .         .         .         .
  T13L16.16 642 ..RKPWAPMKTKQVQHRNHLQKSCK.NANKMVLWFEVDDTGCGIDPSKWD 688
                  :  .   | .   :   :  | : ||| |   ||||||| ||      
     AtCKI1 563 FVKSMFCKNKEESSTYETEISNSIRNNANTMEFVFEVDDTGKGIPMEMRK 612
                         .         .         .         .         .
  T13L16.16 689 SVFESFEQADPSTTRTHGGTGLGLCIVRNLVNKMGGEIKVVQK..NGLGT 736
                ||||.: |    | . | |||||| ||..||  |||||::  |     ||
     AtCKI1 613 SVFENYVQV.RETAQGHQGTGLGLGIVQSLVRLMGGEIRITDKAMGEKGT 661
                         .         .         .         .         .
  T13L16.16 737 LMRLYLILST...PDTVDQNIQPDFSKYGLVVMLSMYGSTARMITSKWLR 783
                  .  .:|.|   |   |  :. :    |  |     | |        : 
     AtCKI1 662 CFQFNVLLTTLESPPVSDMKVRQEIEAGGDYVSTPNLGLTINTSLGGSMN 711
                         .         .         .         .         .
  T13L16.16 784 KHGIATVEASDWNELTQIIRDLLETGSRDNSFDSQHNISDPLRAELSNIV 833
                   :.    .  .   .     .    :.                   :|
     AtCKI1 712 IRNLSPRFNNCLSSSPKQEGSRVVLLLKNEERRRVTEKYIKNLGIKVTVV 761
                         .         .         .         .         .
  T13L16.16 834 EIKNPVFVIVVDIGVLDLTTNIWKEQLNYLDRFSNKAKFAWLLKHDTSNT 883
                |    .   .  :      ... : : .     | .  |  :   |. . 
     AtCKI1 762 EKWEHLSYALERLFGFSPQSSMGRAECSLSCPSSRELPFIGMDGIDSRSQ 811
                         .         .         .         .         .
  T13L16.16 884 VKTELRRKGHVMMVNKPLYKAKMIQILEAVIKNRKRGLCNDLRNRGNGSD 933
                .          ...     |      |  ::|  :||| . :  :    .
     AtCKI1 812 LPKRRSISFSAVVLLVIDAKTGPFFELCDIVKQFRRGLPHGISCKVVWLN 861
                         .         .         .         .         .
  T13L16.16 934 ESHDCLEIDPTQFDTCSSDDSSETSGEKQVDK......SVKPSTLHSPVL 977
                ||   .        .     |      | . .         |. |    |
     AtCKI1 862 ESSTRVSERGDISCSRPLHGSRLMEVLKMLPEFGGTVLKEPPTELQRESL 911
                         .         .         .         .         .
  T13L16.16 978 KNYLIDATTSNDDSTSASMTQKNPEEEDWKDRLYSGIALDGKNQKSLEGI 1027
                  :   |  |               ..  |  : |  .      ||.   
     AtCKI1 912 LRHSFVAERSPKHKVQEEGPSSMFNKKLGKRIMASTDSESETRVKSVRTG 961
                         .         .         .         .         .
  T13L16.16 1028 RILLAEDTPVLQRVATIMLEKMGATVTAVWDGQQAVDSLNYKSINAQAPT 1077
                 |  :       :       | :      | |       .    :     .
     AtCKI1  962 RKPIGNPEDEQETSKPSDDEFLRGKRVLVVDDNFISRKVATGKLKKMGVS 1011
                         .         .         .         .         .
  T13L16.16 1078 EEHKSFEEETANKVTTRETSLRNSS......PYDLILMDCQMPKMDGYEA 1121
                 |  .    . | :. |   . |         |:| | ||||||.||||||
     AtCKI1 1012 EVEQCDSGKEALRLVTEGLTQREEQGSVDKLPFDYIFMDCQMPEMDGYEA 1061
                         .         .         .         .         .
  T13L16.16 1122 TKAIRRAEIGTELHIPIVALTAHAMSSDEAK.CLEVGMDAYLTKPIDRKL 1170
                 |: ||: |    .  ||:|.. |   |:||:  :: ||||:| | :..  
     AtCKI1 1062 TREIRKVEKSYGVRTPIIAVSGHDPGSEEARETIQAGMDAFLDKSLNQLA 1111
                         .         . 
  T13L16.16 1171 MVSTILSLTKPSAFQTSLSA* 1191
                  |   :   :           
     AtCKI1 1112 NVIREIESKRH*          1123

 

written 25 Nov 97
Larry Parnell