The putative zinc-finger protein encoded by T13L16.6 and the putative reverse transcriptase encoded by T13L16.7 may together comprise a putative non-LTR retrotransposon similar to Tal-1.

CDS:  The table below lists the coordinates of the exons for T13L16.6 and which exon predicting algorithms selected the given termini (GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene - selects splice sites only, not exons).

Note:  Translation start at 41533 is based on identity to T32G6.11. The splice site at 40561 is purely conjectural and is selected to maintain the open reading frame, to lengthen the intron of 34 nt (as predicted by GenScan) to 155 nt, and to minimize the amount of protein coding sequence for which there is no computer-based prediction.

Complete CDS of T13L16.6

ATGGCGGATGAGCTTTGGGACGAAATACAGAATTTGGAGTTGAGACAGGAGGGTCCTTCT
TTGTTCATCCCAAATGAAGCATACATCATGGTTGCAGGCCGGAACAGACTTAGCATCATT
GCTCGACCTCTGAATCCAAGGGTCCAAAACCTTCAGGCTATTATTACTGCTCTACCTAGA
GCCTGGGGCTTAACAGCTCATGTTCATGGCAGGATTATAGATGATACATATGTGCAATTC
CTCTTTCAGTCGGAAATGGACCTGCTATCAGTTCAAAGGAGGGAACCCTGGCTTTTCAAC
AATTGGTTTGTCGCATCTCAACGTTGGCAGCCTGCTCCTGCTTTGAACTTTGTGACAACC
ATTGATCTCTGGGTTCAGATGAGAGGTATTCCCTTCCTTTATGTATCTGAAGAAACTGCT
TTAGAAATAGCTCAAGAGATTGGTGCAATCATTTCCTTGGACTTTCATGATACTACATCA
ACTCAGATTGCTTATATTAGAGTTCGAGTTCGTGTTGGTATTACAGACAGTTTAAGGTTC
TTTCAACGCATCACGTTTGAGTCGGGAGAATCAGCTTTAATCAGGTTTCAGTATGAACGT
TTAAGGCGTATTTGTAGTAACTGTTTCAGGTTTACTCACAACAGAAACTACTGTCCTTAC
CGTCCAAGGGTTCAGATTCTAGACAGAGAGAGAGCAGCTTTACATGACAGTGTATTAAGA
TCAAGCAACAACTCTCAGTCTCAGATGACTGAAAGTTCTTTCCCTGCTCCTTTAACCCCT
CCTCCACGAGTGCCTGCACCACCACTGAATCACCAAGAACGGGTTTATGAAACAGGAGAA
TCATCAAGACGTGTTGAGGATAGGGAATCCAACAATAACCTGCACCTTGGCGACTCATCC
AAACGCAAAAACATGATAATCCTACAATGTGAAAAGGATTCACAAGAGAACAAGCAACAA
GAAACAGATCTAAACATGGGAGGTATTCCGAAACCTCCAAAGAAACGCTAA

Protein sequence of T13L16.6

MADELWDEIQNLELRQEGPSLFIPNEAYIMVAGRNRLSIIARPLNPRVQNLQAIITALPR
AWGLTAHVHGRIIDDTYVQFLFQSEMDLLSVQRREPWLFNNWFVASQRWQPAPALNFVTT
IDLWVQMRGIPFLYVSEETALEIAQEIGAIISLDFHDTTSTQIAYIRVRVRVGITDSLRF
FQRITFESGESALIRFQYERLRRICSNCFRFTHNRNYCPYRPRVQILDRERAALHDSVLR
SSNNSQSQMTESSFPAPLTPPPRVPAPPLNHQERVYETGESSRRVEDRESNNNLHLGDSS
KRKNMIILQCEKDSQENKQQETDLNMGGIPKPPKKR*

BLAST output demonstrating identity to T32G6.11

 gi|2618694 (AC002510) putative zinc-finger protein [Arabidopsis thaliana]
           Length = 367
           
 Score =  444 bits (1131), Expect = e-124
 Identities = 229/367 (62%), Positives = 265/367 (71%), Gaps = 31/367 (8%)

Query: 1   MADELWDEIQNLELRQEGPSLFIPNEAYIMVAGRNRLSIIARPLNPRVQNLQAIITALPR 60
           M+DELW+EIQNLEL QE P+LFIP+EAY+MV   NRLS+IARPLNPRVQNL +++ ALPR
Sbjct: 1   MSDELWNEIQNLELGQEDPALFIPHEAYVMVEATNRLSMIARPLNPRVQNLNSVVVALPR 60

Query: 61  AWGLTAHVHGRIIDDTYVQFLFQSEMDLLSVQRREPWLFNNWFVASQRWQPAPALNFVTT 120
            WGLT  VHGRI+D TYVQFLFQ+E+DL+ VQR+EPWLFNNWFVA+ RW+ APA NFVTT
Sbjct: 61  TWGLTNQVHGRILDATYVQFLFQNEIDLMMVQRKEPWLFNNWFVAATRWEVAPAHNFVTT 120

Query: 121 IDLWVQMRGIPFLYVSEETALEIAQEIGAIISLDFHDTTSTQIAYIRVRVRVGITDSLRF 180
           IDLWVQ+RGIP  YVSEET +EIAQ++G ++ LD+HDTTS QIAYIRVRVR GITD LRF
Sbjct: 121 IDLWVQIRGIPLPYVSEETVMEIAQDLGEVLMLDYHDTTSIQIAYIRVRVRFGITDRLRF 180

Query: 181 FQRITFESGESALIRFQYERLRRICSNCFRFTHNRNYCPYRPRVQILDRERAALHDSVLR 240
           FQRI F+SGE+A IRFQYERLRRICS+CFRFTHNR YCPYRPR   + RERA   DSV R
Sbjct: 181 FQRIVFDSGETATIRFQYERLRRICSSCFRFTHNRAYCPYRPRPLSIARERALFRDSVHR 240

Query: 241 SSNNSQSQMTESSFPAPLTPPPRVPAPPLNHQERVYE--------------TGESSRRVE 286
           SS NSQSQMT+SSFP P TPPPR+  PPLNH E V                 GESS   +
Sbjct: 241 SSMNSQSQMTDSSFPIPQTPPPRISHPPLNHDEFVAAYPHLDSGRNDHIRCEGESSNFRQ 300

Query: 287 DRESNNN----------------LHLGDSSKRKNMIILQCEKDSQEN-KQQETDLNMGGI 329
           D  S +N                   G SS+R ++  L+   +   N  QQ      GGI
Sbjct: 301 DLSSASNSITPRREPQYLTDRRHFEPGQSSRRHDVRDLRRGSERIGNLNQQNYVQRSGGI 360

Query: 330 PKPPKKR 336
            KPPKKR
Sbjct: 361 LKPPKKR 367

written 29 Dec 97
Larry Parnell