Gene T17A5.1
Putative Identification serine acetyltransferase
Position 75979 to 77758, from the initial methionine to the termination codon
Strand +
EST match T43736
Database match plant serine acetyltransferases

 

CDS:  The table below lists the coordinates of the T17A5.1 exons and which exon prediction program selected the 5' and 3' termini (GF = Genefinder, GS = GenScan, Gr = Grail, M = MZEF, NPG = NetPlantGene - selects splice sites, not exons). Splice junctions determined by identity to EST T43736 are designated by EST.

Exon Range 3' 5'
1 75979 to 76119 GF,GS ESTGF,GS,Gr,NPG
2 76377 to 76574 EST,GF,GS,Gr,M,NPG GF,GS,Gr,M,NPG
3 76648 to 76707 GF,GS,Gr,NPG GS,Gr
4 76793 to 76895 NPG GF
5 77141 to 77235 GF,GS,Gr,M,NPG GF,GS,Gr,NPG
6 77335 to 77501 GS,Gr,M,NPG GF,M,NPG
7 77587 to 77660 GF,M,NPG GF,GS,Gr,M,NPG
8 77724 to 77758 GF GF

Complete CDS of T17A5.1

ATGAATGGCGATGAGCTTCCTTTCGAGAGTGGTTTCGAGGTTTACGCTAAGGGAACTCAT
AAGTCAGAGTTTGACTCGAATTTGCTTGATCCTCGTTCTGATCCTATTTGGGATGCTATA
AGAGAAGAAGCTAAACTTGAGGCAGAGAAAGAGCCTATTTTGAGTAGCTTCTTGTATGCT
GGTATCTTAGCACATGATTGTTTAGAGCAAGCTTTAGGGTTTGTTCTAGCCAACCGTCTC
CAAAACCCAACCTTGTTGGCAACACAACTCTTGGATATATTTTATGGTGTTATGATGCAT
GACAAAGGTATTCAGAGTTCGATTCGCCATGATCTCCAGGCATTTAAAGATCGTGATCCT
GCTTGTCTGTCGTATAGTTCTGCTATTTTACATCTGAAGGGTTATCATGCGTTACAAGCA
TATAGGGTTGCGCATAAACTGTGGAATGAAGGGAGGAAACTATTAGCTCTTGCATTGCAA
AGCCGAATAAGCGAGGTAGTTGCGGCAAGAATTGGGGAGGGAATATTGTTGGATCATGGA
ACTGGAGTGGTCATTGGTGAGACCGCTGTGATAGGCAACGGTGTCTCGATCTTACATGGT
GTGACTTTAGGAGGAACCGGAAAGGAAACTGGCGATCGCCACCCAAAGATAGGTGAAGGT
GCATTGCTTGGAGCTTGTGTGACTATACTTGGTAACATAAGCATAGGTGCTGGAGCAATG
GTAGCTGCAGGTTCACTTGTGTTAAAAGACGTTCCTTCGCATAGTGTGGTGGCTGGAAAT
CCTGCAAAACTGATCAGGGTCATGGAAGAGCAAGACCCGTCTCTAGCAATGAAACACGTG
ACTGTTCTTGAACGAATATGCAGATGCTACTAA

 

Protein sequence:

MNGDELPFESGFEVYAKGTHKSEFDSNLLDPRSDPIWDAIREEAKLEAEKEPILSSFLYA
GILAHDCLEQALGFVLANRLQNPTLLATQLLDIFYGVMMHDKGIQSSIRHDLQAFKDRDP
ACLSYSSAILHLKGYHALQAYRVAHKLWNEGRKLLALALQSRISEVVAARIGEGILLDHG
TGVVIGETAVIGNGVSILHGVTLGGTGKETGDRHPKIGEGALLGACVTILGNISIGAGAM
VAAGSLVLKDVPSHSVVAGNPAKLIRVMEEQDPSLAMKHVTVLERICRCY*

 

Alignment of the best database matches to T17A5.1. The aligned sequences include serine acteyltransferases from watermelon, spinach, and Arabidopsis (clone #52). 

               1                                                         60
WatermelonSAT  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MPVGELR 
   SpinachSAT  MLAVSYAIPSCCINRFYIREFSSSLQPALHHPWYKKRFGCCSTNQENCSLGSPTHLPRMP 
      AtSAT52  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MPPAGELRHQSPSKEKLSSVTQSD 
      T17A5.1  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MNGDELPFESGFEVY 

               61                                                       120
WatermelonSAT  FSSQSSTTVVESTTNNDETWLWGQIKAEARRDAESEPALASYLYSTILSHSSLERSLSFH 
   SpinachSAT  GGDLSVAPSVGHLTANNEAWLWDQIKGEARRDADSEPALASYLYSTILSHSSLERSLSFH 
      AtSAT52  EAEAASAAISAAAADAEAAGLWTQIKAEARRDAEAEPALASYLYSTILSHSSLERSISFH 
      T17A5.1  AKGTHKSEFDSNLLDPRSDPIWDAIREEAKLEAEKEPILSSFLYAGILAHDCLEQALGFV 

               121                                                      180
WatermelonSAT  LGNKLCSSTLLSTLLYDLFLNAFSTDYCLRSAVVADLQAARERDPACVSFSHCLLNYKGF 
   SpinachSAT  LGNKLCSSTLLSTLLYDLFLNILSSDSSLLDAVVADLRAARVRDPACVSFSHCLLNYKGF 
      AtSAT52  LGNKLCSSTLLSTLLYDLFLNTFSSDPSLRNATVADLRAARVRDPACISFSHCLLNYKGF 
      T17A5.1  LANRLQNPTLLATQLLDIFYGVMMHDKGIQSSIRHDLQAFKDRDPACLSYSSAILHLKGY 

               181                                                      240
WatermelonSAT  LACQAHRVAHKLWNQSRRPLALALQSRIADVFAVDIHPAARIGKGILFDHATGVVVGETA 
   SpinachSAT  LACQSHRVAHKLWNQDRRPLALALHSRISDVFAVDIHPAARIGKGILFDHATGVVIGETA 
      AtSAT52  LAIQAHRVSHKLWTQSRKPLALALHSRISDVFAVDIHPAAKIGKGILLDHATGVVVGETA 
      T17A5.1  HALQAYRVAHKLWNEGRKLLALALQSRISEVV......AARIGEGILLDHGTGVVIGETA 

               241                                                      300
WatermelonSAT  VIGNNVSILHHVTLGGTGKMCGDRHPKIGDGVLIGAGATILGNVKIGEGAKIGAGSVVLI 
   SpinachSAT  IIGDNCSILHHVTLGGTGKAGGDRHPKVGDGVLIGAGATILGNVRIGDGAKIGAGSVVLI 
      AtSAT52  VIGNNVSILHHVTLGGTGKACGDRHPKIGDGCLIGAGATILGNVKIGAGAKVGAGSVVLI 
      T17A5.1  VIGNGVSILHGVTLGGTGKETGDRHPKIGEGALLGACVTILGNISIGAGAMVAAGSLVLK 

               301                                           349
WatermelonSAT  DVPPRTTAVGNPARLVGGKEKPS.QLEDIPGESMDHTSFISEWSDYII*
   SpinachSAT  DVPPRTTAVGNPARLIGGKEKPS.QNSDVPGESMDHTSFISEWSDYII*
      AtSAT52  DVPCRGTAVGNPARLVGGKEKPTIHDEECPGESMDHTSFISEWSDYII*
      T17A5.1  DVPSHSVVAGNPAKLIRVMEEQDPSLAMKHVTVLERICRCY*~~~~~~~

 

created 1 Nov 97
Larry Parnell