CDS:  The table below lists the coordinates of the T17A5.12 exons and which exon prediction algorithms selected the 3' and 5' termini (GF = Genefinder, GS = GenScan, Gr = Grail, M = MZEF, NPG =NetPlantGene - selects splice sites, not exons).

Complete CDS of T17A5.12

ATGAGATTCTTGTTAACCAAAAGAGCATTCAGAATCTTCAACCCACGTTTCCAGAGACTG
TGGTTAACTTCTTCTCCCTTCTCCTCAACCTCAAATTCCGGCGGATTTCCCGACGATTCC
GAGCCGGAATCATGGAGAACTATAGAGGGTCTTCTCCGTTCCCCTGCAAATTTCTCTCCT
TTATCTCCAATCACGTTCTTGGAGCGATCCGCTAAGGTTTACAGAGACAGAACCTCTCTT
GTGTTTGGTTCCGTTAAACACACTTGGTTCCAAACTTATCAACGTTGTCTCCGTCTTGCC
TCTGCTCTTACCAATCTCGGAATCTCTCGTGGCGATGTGGTTGCAGCTTTAGCTCCGAAT
GTTCCAGCTATGCATGAGCTTCATTTCGCTGTTCCTATGGCTGGTTTGATTCTTTGTCCG
CTTAATACTCGACTTGATCCTTCCACATTGTCGGTTTTGTTAGCACACTCCGAGGCCAAA
ATCCTCTTTGTTGATCATCAGTTACTTGAGATTGCTCATGGAGCTCTTGATCTTCTTGCT
AAATCAGATAAAACTAGAAAAAGTCTGAAGCTTGTGTTGATCTCTCAGTCTAATGATGAT
GATGATAGTGATGAAGATAGCTCATCTACCTTTGCTTCAAAGTACTCTTTTGATTACGAA
TATGAAACTCTGCTTAAATCCGGAGATAGCGAGTTTGAGATAATCAAACCGAGATGCGAA
TGGGATCCTATTAGTATAAACTACACTTCAGGGACGACTTCGAGACCTAAGGGTGTAGTG
TATAGCCATAGAGGAGCTTATCTCAATTCTCTTGCTACAGTCTTTCTTCACCAGATGTCT
GTTTATCCGGTGTATTTATGGACAGTGCCGATGTTTCACTGTAACGGATGGTGTCTTGTT
TGGGGAGTAGCAGCTCAAGGTGGTACTAATATCTGTCTCAGGAAAGTCTCTCCTAAGATG
ATCTTTAAGAACATTGCTATGCATAAAGTGACTCACATGGGAGGAGCCCCAACTGTGTTG
AATATGATTGTGAACTATACTGTGACTGAACATAAACCGCTTCCTCACAGGGTTGAGATC
ATGACAGGTGGGTCACCGCCTCTACCGCAGATCCTGGCTAAGATGGAAGAATTAGGTTTC
AATGTGTCTCATCTTTATGGTTTGACAGAGACATACGGTCCAGGGACACATTGCGTGTGG
AAACCTGAATGGGATTCACTTTCGTTGGAGGAGAGAACTAAGTTGAAGGCTAGACAAGGA
GTGCAGCATTTGGGTCTAGAAGGGCTCGATGTGAAAGATCCGCTGACAATGGAGACTGTC
CCTGATGATGGTTTAACCATGGGTGAGGTTATGTTCAGAGGAAACACTGTGATGAGTGGA
TATTTCAAGGACATAGAGGCAACACGAAAAGCTTTCGAGGGAGATTGGTTCCACAGTGGT
GATCTCGCTGTTAAGTATCCAGACGGGTACATAGAGATAAAAGACCGGTTAAAAGATGTG
ATCATCTCAGGAGGAGAAAACATAAGCTCAGTGGAGGTTGAGAGAGTTTTGTGCAGCCAT
CAAGCGGTTCTTGAAGCTGCTGTTGTAGCACGTCCAGATCATCACTGGGGACAGACTCCT
TGCGGTTTTGTAAAGCTGAAAGAAGGATTTGATACCATCAAACCCGAGGAGATTATCGGG
TTCTGTCGAGATCATTTGCCGCATTACATGGCTCCAAAGACTATAGTTTTCGGGGACATA
CCTAAAACCTCGACGGGGAAAGTACAAAAGTATCTTCTGAGGAAGAAAGCTGATGAAATG
GGTAGCTTGTAA

Protein sequence:

MRFLLTKRAFRIFNPRFQRLWLTSSPFSSTSNSGGFPDDSEPESWRTIEGLLRSPANFSP
LSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLGISRGDVVAALAPN
VPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIAHGALDLLA
KSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEFEIIKPRCE
WDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLWTVPMFHCNGWCLV
WGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEHKPLPHRVEI
MTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKLKARQG
VQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKAFEGDWFHSG
DLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAAVVARPDHHWGQTP
CGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQKYLLRKKADEM
GSL*

Alignment of T17A5.12 and B. napus AMP-binding acyl-CoA synthetase ACSF8. The two proteins are 50% identical. The AMP-binding site is highlighted.

          1                                                         60
 BnACSF8  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MELLLPHPSNSSP 
T17A5.12  MRFLLTKRAFRIFNPRFQRLWLTSSPFSSTSNSGGFPDDSEPESWRTIEGLLRSPANFSP 

          61                                                       120
 BnACSF8  LTVLGFLDRAAAVYGDSPSLLHDTTTHTWSETHSRCLRVASTLSSSSLGINRGQVVSVIG 
T17A5.12  LSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASAL..TNLGISRGDVVAALA 

          121                                                      180
 BnACSF8  PNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVVLRHSESKLVFVDHHSSSLVLEALSF 
T17A5.12  PNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIAHGALDL 

          181                                                      240
 BnACSF8  LPKNEKPR....LVLLQDDSNISGLTNMSASSSADVDFLD.TYEGVMERGDPRFKWVRPN 
T17A5.12  LAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEFEIIKPR 

          241                                                      300
 BnACSF8  NEWNPMVLNYTSGTTSSPKGVVLSHRAVFMSTVNSLLYWSMPNRPVYLWTLPMFHANGWG 
T17A5.12  CEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLWTVPMFHCNGWC 

          301                                                      360
 BnACSF8  YTWATAAVGATNICVRRVDAPTIFELIDKHQVTHMCAAPMVLNMLTNY..PSRKQLKRPV 
T17A5.12  LVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEHKPLPHRV 

          361                                                      420
 BnACSF8  QIMTAGAPPPATVISSAESLGFDVAHGYGLTETGGLVVTCAWKPEWDGLGQDERARLKSR 
T17A5.12  EIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKLKAR 

          421                                                      480
 BnACSF8  QGIRTAVFAEADVLDTLTGKSVKHDGVTVGEIVFRGGCVMLGYYNDPEGTAASMREDGWF 
T17A5.12  QGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKAFEGD.WF 

          481                                                      540
 BnACSF8  YTGDIGVMHPDGYLEVKDRSKDVIICGGENISSTEVETVLYTNPAVKEAAVVAKPDKMWG 
T17A5.12  HSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAAVVARPDHHWG 

          541                                                      600
 BnACSF8  ETPCAFVSLKCSDRGSVTEREVREFCKKKLPKYMVPRNVVFMEELPKTSTGKIQKFLLRQ 
T17A5.12  QTPCGFVKLK.EGFDTIKPEEIIGFCRDHLPHYMAPKTIVF.GDIPKTSTGKVQKYLLRK 

          601  
 BnACSF8  MAKSLP*~~
T17A5.12  KADEMGSL*

created 1 Nov 97
Larry Parnell