Gene T17A5.2
Putative Identification protein kinase
Position 56927 to 61991, from the initial methionine to the termination codon
Strand +
EST match T43472 and T20930
Database match soybean PK6

 

CDS:  The table below lists the coordinates of the T17A5.2 exons and which exon prediction algorithm selected the 5' or 3' terminus (GF = Genefinder, GS = GenScan, Gr = Grail, M = MZEF, NPG = NetPlantGene - selects splice sites, not exons). Splice sites determined by identity to an EST are designated as EST.

Exon Range 5' 3'
1 56927 to 57129 GS,Gr GS,NPG
2 57441 to 57555 GS,Gr,M,NPG GS,Gr,M,NPG
3 57634 to 57713 GS,Gr,M,NPG GS,Gr,M,NPG
4 57811 to 57921 GS,M,NPG GS,M,NPG
5 58036 to 58090 GS,Gr,M,NPG EST,GF,GS,Gr,NPG
6 58237 to 58347 EST,GF,GS,Gr,M,NPG EST,GF,GS,Gr,M,NPG
7 58450 to 58500 EST,GF,Gr,M,NPG EST,GF,Gr,M,NPG
8 58652 to 58824 EST,GF,GS,Gr,M,NPG EST,GF,GS,Gr,M,NPG
9 58913 to 59020 EST,GF,Gr,M,NPG EST,M
10 59251 to 59324 EST,GS,NPG GS,NPG
11 59406 to 59581 NPG GF,Gr,NPG
12 59700 to 59830 GS,NPG GS,Gr,M,NPG
13 59955 to 60035 GS,Gr GF,GS,Gr,NPG
14 60128 to 60181 GS,Gr,M,NPG GS,Gr,M,NPG
15 60275 to 60409 GF,GS,Gr,M,NPG GS,Gr,M
16 61154 to 61254 GS,Gr,M,NPG GS,Gr,M,NPG
17 61359 to 61535 GS,Gr,M,NPG GS,Gr,M,NPG
18 61610 to 61991 GS,Gr,M,NPG GS

Complete CDS of T17A5.2

ATGACGATCAAAGATGAGTCGGAGAGTTGCGGTAGCAGAGCCGTCGTTGCTTCGCCGTCA
CAAGAAAACCCTAGACATTACCGGATGAAACTTGATGTCTATAGTGAGGTTTTACAGCGA
CTTCAAGAATCTAATTACGAAGAAGCCACTCTTCCTGATTTCGAGGATCAACTTTGGCTC
CATTTCAATCGTCTTCCTGCTCGATATGCTCTTGATGTTAAAGTCGAGAGAGCGGAAGAT
GTTCTCACACATCAGAGATTGCTAAAATTGGCTGCAGATCCTGCTACTAGGCCTGTCTTT
GAAGTTCGAAGTGTACAGGTTTCTCCCAGAATCTCTGCTGACTCTGACCCTGCGGTGGAG
GAAGATGCTCAAAGCTCTCACCAACCAAGCGGACCGGGGGTTCTTGCTCCTCCAACTTTT
GGTTCTTCTCCAAATTTTGAGGCTATTACTCAGGGAAGTAAAATTGTTGAAGATGTTGAT
AGTGTTGTGAATGCAACATTGTCTACACGACCGATGCACGAGATCACTTTTTCAACCATT
GATAAACCGAAACTCCTTAGCCAGCTAACTTCCCTGCTTGGTGAGCTTGGACTGAATATA
CAAGAGGCTCATGCTTTTTCCACTGTAGATGGTTTCTCTTTAGATGTCTTTGTAGTTGAC
GGTTGGTCTCAGGAGGAAACTGATGGCCTAAGAGATGCATTGAGCAAAGAAATACTGAAG
CTTAAGGATCAACCTGGTTCAAAACAGAAATCTATTTCTTTCTTTGAGCATGACAAATCA
AGCAATGAGCTTATACCCGCCTGCATTGAAATACCCACGGATGGAACTGATGAGTGGGAA
ATCGACGTGACTCAGCTCAAAATTGAAAAGAAAGTGGCATCTGGTTCATATGGGGATCTG
CATAGAGGCACTTATTGCAGTCAGGAAGTAGCTATCAAATTTCTCAAGCCTGATCGTGTA
AACAATGAGATGCTGAGAGAATTTTCTCAAGAAGTTTTTATAATGAGGAAAGTTCGACAC
AAAAACGTCGTTCAATTTTTGGGTGCATGCACAAGATCTCCAACCCTCTGTATAGTGACT
GAGTTTATGGCTCGAGGGAGCATATATGATTTTCTTCACAAACAGAAATGCGCTTTCAAA
CTTCAAACTTTACTCAAAGTTGCACTTGATGTCGCAAAAGGAATGAGCTATTTGCATCAA
AACAACATTATTCACAGGGACCTTAAGACTGCGAATCTTCTTATGGATGAACATGGACTT
GTCAAGGTTGCTGATTTCGGAGTTGCCAGAGTGCAGATTGAATCAGGGGTCATGACTGCT
GAAACTGGGACATACCGGTGGATGGCTCCAGAGGTCATTGAGCACAAACCTTACAATCAC
AAGGCAGATGTGTTCAGTTATGCGATAGTGCTATGGGAACTTCTGACTGGTGACATCCCA
TATGCTTTCTTGACTCCACTACAAGCAGCTGTTGGCGTTGTCCAAAAGGGGCTTCGACCC
AAAATCCCAAAGAAAACACACCCAAAAGTGAAGGGGCTTCTAGAGAGATGCTGGCATCAA
GACCCAGAACAGAGACCACTGTTTGAGGAAATCATAGAAATGCTACAACAGATAATGAAA
GAGCCGGTGACTGTTTTTGGGTCTGCTTCAATTGCAGTGGAAGAGATGGTGTTCTTGAGT
TGGGGCCGTCCATCTTCCGAGCAACAGCAACAAGTCATCAACAAAACAGGAACCTTCAAT
TATGACAACAAGTACAGAGGAGTTTCGTCTAGGTCTATTGCTAAACTTAAGGAAGATTCA
GAGATTGATAAAGATGGATTCTTGATCAATCATGCTCGTGTTTTAGTCGGTTCTGGTAGA
GAGAGTTATGAGAAGGGCAAAAAAGCTCTCCAGAATTGGAAGCATTTTGGTATGGATTGG
GCATTTGTTGATCCTGCAACACCTGTTGAAACCGGTAAGAAGTTTTGCATTTGCGTGAAA
GAAGTTCTTCCATGGGTGATGCTTCCTCTACAAGTGGTTTATGTTGACGAAAGCCGGAAA
TCAAGAAAAGGCCCTGCGCATTTCGGTTACGGAAGCGGTACTCTTCAGGGACATTTACTC
GCTGGAGAAGAAAAGTTTTCGATAGAGCTTGATGGAAATGGTGAGGTATGGTATGAGATA
ACGTCTTTCTCAAAGCCTGCTCATTTCTTGTCGTTCCTCGGGTATCCTTATGTGAAGCTA
AGGCAGAAGCACTTTGCTCGTCATTCTTCTGAAGCTGTGCTGAAACATGTCAATGCTTCG
TGA

 

Protein sequence:

MTIKDESESCGSRAVVASPSQENPRHYRMKLDVYSEVLQRLQESNYEEATLPDFEDQLWL
HFNRLPARYALDVKVERAEDVLTHQRLLKLAADPATRPVFEVRSVQVSPRISADSDPAVE
EDAQSSHQPSGPGVLAPPTFGSSPNFEAITQGSKIVEDVDSVVNATLSTRPMHEITFSTI
DKPKLLSQLTSLLGELGLNIQEAHAFSTVDGFSLDVFVVDGWSQEETDGLRDALSKEILK
LKDQPGSKQKSISFFEHDKSSNELIPACIEIPTDGTDEWEIDVTQLKIEKKVASGSYGDL
HRGTYCSQEVAIKFLKPDRVNNEMLREFSQEVFIMRKVRHKNVVQFLGACTRSPTLCIVT
EFMARGSIYDFLHKQKCAFKLQTLLKVALDVAKGMSYLHQNNIIHRDLKTANLLMDEHGL
VKVADFGVARVQIESGVMTAETGTYRWMAPEVIEHKPYNHKADVFSYAIVLWELLTGDIP
YAFLTPLQAAVGVVQKGLRPKIPKKTHPKVKGLLERCWHQDPEQRPLFEEIIEMLQQIMK
EPVTVFGSASIAVEEMVFLSWGRPSSEQQQQVINKTGTFNYDNKYRGVSSRSIAKLKEDS
EIDKDGFLINHARVLVGSGRESYEKGKKALQNWKHFGMDWAFVDPATPVETGKKFCICVK
EVLPWVMLPLQVVYVDESRKSRKGPAHFGYGSGTLQGHLLAGEEKFSIELDGNGEVWYEI
TSFSKPAHFLSFLGYPYVKLRQKHFARHSSEAVLKHVNAS*

 

Alignment of T17A5.2 and SoyPK6. There is significant sequence conservation within elements of the general kinase motif (noted by Roman numerals, after Hanks et al., 1988), but notable differences between the two sequences indicate that these two kinases likely have distinct biochemical properties. 

                  .         .         .         .         .
T17A5.2     101 EVRSVQVSPRISADSDPAVEEDAQSSHQPSGPGVLAPPTFGSS..PNFEA 148
                                              |   :    |  .     : 
 SoyPK6       1 ..........................MGEDGNSWIRRTNFSHTVCHRLDP 24
                  .         .         .         .         .
T17A5.2     149 ITQGSKIVEDVDSVVNATLSTRPMHEITFSTIDKPKLLSQLTSLLGELGL 198
                   ||  :       .   |    | .|:       |    | |      
 SoyPK6      25 ARLGSIPISVQSEQKSRPSSKAQRHPMTYKQRSLSPLPE..TYLSEAFRE 72
                  .         .         .         .         .
T17A5.2     199 NIQEAHAFSTVDGFSLDVFVVDGWSQEETDGLRDALSKEILKLKDQPGSK 248
                   |   ||| .       .    ..:  :    .          |  ||
 SoyPK6      73 ARLEQKRFSTPNPRREKRIMGKLLNKDSRETKESSSKSPSRSPNRQVKSK 122
                  .         .         .         .         .
T17A5.2     249 QKSISFFEHDKSSNELIPACIEIPTDGTDEWEIDVTQLKIEKKVASGSYG 298
                 :  | .     .       :|      :|| :|..||    | | |.: 
 SoyPK6     123 NRKDSAWTKLLDNGGGKITAVET....AEEWNVDMSQLFFGLKFAHGAHS 168

                  .         II         .         .    III     .
T17A5.2     299 DLHRGTYCSQEVAIKFLK.PDRVNNEML.....REFSQEVFIMRKVRHKN 342
                 |: | |  : ||:| :  |:   |  |     ::| .|| :: :. |.|
 SoyPK6     169 RLYHGVYKDEAVAVKIIMVPEDDGNGALASRLEKQFIREVTLLSRLHHQN 218

                  .         .  IV       .         .         .    V
T17A5.2     343 VVQFLGACTRSPTLCIVTEFMARGSIYDFLHK.QKCAFKLQTLLKVALDV 391
                |:.|  || : |  ||:||::| ||:  :||| :     || |:  |||:
 SoyPK6     219 VIKFSAACRKPPVYCIITEYLAEGSLRAYLHKLEHQTISLQKLIAFALDI 268

                  .         . VI        .         .VII         .
T17A5.2     392 AKGMSYLHQNNIIHRDLKTANLLMDEHGLVKVADFGVARVQIESGVMTAE 441
                |:|| |:|   :||||||  |:|..|   .|:||||:|  :    .:  :
 SoyPK6     269 ARGMEYIHSQGVIHRDLKPENILINEDNHLKIADFGIACEEASCDLLADD 318

                  . VIII        .         IX         .         .
T17A5.2     442 TGTYRWMAPEVIEHKPYNHKADVFSYAIVLWELLTGDIPYAFLTPLQAAV 491
                 |||||||||.|. | |  | ||:|: ::|||:||| |||  : |:||| 
 SoyPK6     319 PGTYRWMAPEMIKRKSYGKKVDVYSFGLILWEMLTGTIPYEDMNPIQAAF 368

                  .         .         .         .XI         .
T17A5.2     492 GVVQKGLRPKIPKKTHPKVKGLLERCWHQDPEQRPLFEEIIEMLQQIMKE 541
                 || |  || ||    | .: |:|.||   |:.|| | :::..|:|  . 
 SoyPK6     369 AVVNKNSRPIIPSNCPPAMRALIEQCWSLQPDKRPEFWQVVKILEQ.FES 417
                  .         .         .         .         .
T17A5.2     542 PVTVFGSASIAVEEMVFLSWGRPSSEQQQQVINKTGTFNYDNKYRGVSSR 591
                 .   |. |:          |     |.   :..        |:      
 SoyPK6     418 SLASDGTLSLVPNPCWDHKKGLLHWIQKLGPLHQNSGPVPKPKFT*.... 463

 

created 31 Oct 97
Larry Parnell