CDS:  The table below lists the coordinates of the T2H3.3 exons and which exon prediction algorithms selected the 5' and 3' termini (GS = GenScan, Gr = GRAIL, M = MZEF, NPG = NetPlantGene - selects splice sites only, not exons).

Alternate exons not used in building the gene model:   GRAIL does not predict any model exons in this region. Gene modeling was performed with shadow exons. GRAIL predicts exon 1 from 35988 to 36125. GRAIL splits intron 1 to give an exon from 36258 to 36282. NetPlantGene predicts several splice sites in this region but none have significant confidence scores.

Complete CDS of T2H3.3

ATGGTTTCGTTGAAGCTCCAGAAGCGTCTCGCATCCTCAGTACTCAAGTGCGGGAAGAGA
AAAGTATGGCTCGACCCGAACGAAGGAAGTGATATCTCCATGGCCAATTCGCGTCAAAAC
ATCAGGAAGCTTGTGAAGGATGGATTCATCATCAGGAAGCCAACGAAGATCCACTCACGA
TCCAGGGCACGTCAATTGAATATAGCCAAGAGAAAGGGACGTCACTCTGGTTATGGTAAG
AGGAAGGGTACCCGAGAGGCAAGGTTGCCAACAAAAGTTTTGTGGATGAGGAGGATGAGA
GTGCTGAGGCGTCTGTTGAAGAAGTATAGGGAGACCAAGAAGATTGACAGACATATGTAC
CATGACATGTACATGAAAGTCAAGGGAAACGTCTTCAAGAACAAGCGTGTGTTGATGGAA
AGTATTCACAAATCCAAGGCTGAGAAGGCTAGAGAGAAGACATTGTCTGACCAGTTTGAG
GCTAAGAGGGCTAAGAACAAGGCTAGCCGAGAGAGGAAACATGCTCGACGAGAGGAGCGT
CTTGCCAAGGGTCCTGGAGGAGACATACCTGCCGCTGCACCACCTGCACAAACCGCTGAG
GTACCTGCGAAAAAGTCTAAGAAGTGA

Protein translation of T2H3.3

MVSLKLQKRLASSVLKCGKRKVWLDPNEGSDISMANSRQNIRKLVKDGFIIRKPTKIHSR
SRARQLNIAKRKGRHSGYGKRKGTREARLPTKVLWMRRMRVLRRLLKKYRETKKIDRHMY
HDMYMKVKGNVFKNKRVLMESIHKSKAEKAREKTLSDQFEAKRAKNKASRERKHARREER
LAKGPGGDIPAAAPPAQTAEVPAKKSKK*

Multiple sequence analysis of L19 proteins

L19 sequences from several species are aligned here. Some of these sequences are partial. From top to bottom, the source organisms for the aligned sequences are rat, human, mouse, D. melanogaster, A. thaliana T2H3.3, previously described partial sequence of A. thaliana, Z. mays, D. discoideum, C. elegans, S. pombe, S. cerevisiae, Methanococcus vannielii, Mathanococcus jannaschii, Methanobacterium thermoautotrophicum, Archaeoglobus fulgidus, Pyrococcus horikoshii, Haloarcula marismortui, Sulfolobus acidocaldarius and A. thaliana L19-like.

             1                                                         60
     RatL19  ~~MSMLRLQKRLASSVLRCGKKKVWLDPNETNEIANANSRQQIRKLIKDGLIIRKPVTVH 
   HumanL19  ~~MSMLRLQKRLASSVLRCGKKKVWLDPNETNEIANANSRQQIRKLIKDGLIIRKPVTVH 
   MouseL19  ~~MSMLRLQKRLASSVLRCGKKKVWLDPNETNEIANANSRQQIRKLIKDGLIIRKPVTVH 
 DmelanoL19  ~~MSSLKLQKRLAASVLRCGKKKVWLDPNEINEIANTNSRQNIRKLIKDGLIIKKPVVVH 
     T2H3.3  ~~MVSLKLQKRLASSVLKCGKRKVWLDPNEGSDISMANSRQNIRKLVKDGFIIRKPTKIH 
   AthalL19  ~~~~~LKLQKRLRSSVLKCGKRKVWLDPNEGSDISMANSRQNIRKLVKDGFIIRKPTKIH 
   ZmaysL19  ~~~~~LKLQKRLAASYLKCGKGKVWLDPNEVSEISMANSRQNIRKLVKDGFIIKKPHKIH 
DdiscoidL19  ~~MVSLKLQKRLAASILKCGKGRVWIDPNEIADVAMANSRDNVRRLIATGFIMRKPVVVH 
CelegansL19  ~~MSNLRLQKRLASAVLKCGKHRVWLDPNEVSEISGANSRQSIRRLVNDGLIIRKPVTVH 
  SpombeL19  ~~MANLRTQKRLAASVLKCGKRKVWMDPNEISEISNANSRQNVRKLIKDGLVIRKPNLMH 
ScerevisL19  ~~MANLRTQKRLAASVVGVGKRKVWLDPNETSEIAQANSRNAIRKLVKNGTIVKKAVTVH 
  MvanniL19  ~~~MDVSTQRRIAAAVLDCGIDRVWVDPENLEKVKMAITKDDIRLLINDGIIVKKQEKGI 
  MjannaL19  MIIMDVSVQRRMAAEILKCGIERVWIDPTQLDRVKMAMSKDDIRALIKEGVIKKKQKKGI 
 MthermoL19  ~~~MNLTTQKRLAADILKVGVNRIWIDPERIDEVSRAITRDGVKQLIKDGAIKAKPKKGI 
  AfulgiL19  MMVMDLSLQRRLAASVLKCGENRVWFDPAALEDIATAATKQDIRELIEQGVIKRKPVNGV 
 PhorikoL19  ~~MNTLKMQRRIAAELLKCGENRVWIDPEKVDEVASAITREDIRRLIKEGVIRKKPIEGQ 
  HmarisL19  ~~MTDLSAQKRLAADVLDVGKNRVWFNPERQGDIADAITREDVRELVDEGAIQAKDKKGN 
  SacidoL19  ~~MPEFQLQRRLAADIAGVGLNNIKFNPERLEEVEEALTREDIKKLIKERAVIVNPKRGI 
  AthalL19a  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 

             61                                                       120
     RatL19  SRARCRKNTLARR.KGRHMGIGKRKGTANARMPEKVTWMRRMRILRRLLRRYRESKKIDR 
   HumanL19  SRARCRKNTLARR.KGRHMGIGKRKGTANARMPEKVTWMRRMRILRRLLRRYRESKKIDR 
   MouseL19  SRARCRKNTLARR.KGRHMGIGKRKGTANARMPEKVTWMRRMRILRRLLRRYRESKKIDR 
 DmelanoL19  SRYRVRKNTEARR.KDRHCGFGKRKGTANARMPTKLLWMQRQPFCRRLLKKYRDSKKIDR 
     T2H3.3  SRSRARQLNIAKR.KGRHSGYGKRKGTREARLPTKVLWMRRMRVLRRLLKKYRETKKIDR 
   AthalL19  SRSRARQLNIAKR.KGRHSGYGKRNGTREARLPTKVLWMRRMRVLRRLLKKYRETKKIDK 
   ZmaysL19  SRSRCKK~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
DdiscoidL19  SRSRAREHNAAKR.LGRHRGAGNRLGTREARLPSKILWIRRIRVLRRLLKKYREAKKIDK 
CelegansL19  SRFRAREYEEARR.KGRHTGYGKRRGTANARMPEKTLWIRRMRVLRNLLRRYRDAKKLDK 
  SpombeL19  SRFRIRKTHAAKR.LGRHTGYGKRKGTAEARMPSAVVWMRRQRVLRRLLRKYRESGKIDK 
ScerevisL19  SKSRTRAHAQSKR.EGRHSGYGKRKGTREARLPSQVVWIRRLRVLRRLLAKYRDAGKIDK 
  MvanniL19  SSARKKEVQEQKR.KGKRKGPGSRRGAKGARTPKKEKWMNTIRPLRTLLKELRENEKIER 
  MjannaL19  SSARVKKLKEQRK.KGRRRGPGSRRGAAGARTPPKERWMATIRALRKTLKQLRDSGKIDR 
 MthermoL19  SSYRSKKIAQQKK.KGRRRGPGSIKGAKGARRPKKDEWMTTIRALRKDLKEMRDNREINK 
  AfulgiL19  SRARINKRKLQKR.KGRRRGHGSRKGAKGARMPRKRMWILRIRALRKALRQMKAEGVVDR 
 PhorikoL19  SRYRARIRHEQKK.KGRHRGPGSRKGKKTARMGKKELWIKTIRALRRELRKLKEQKKIDR 
  HmarisL19  SRGRARERQKKRA.YGHQKGAGSRKGKAGARQNSKEDWESRIRAQRTKLRELRDEGTLSS 
  SacidoL19  SSGRLKERKHKRRSKGEGRKHGSRKGKSGARTGDKEIWINKIRKIRRYIRWLRDNNVIDK 
  AthalL19a  ~~~~~~~~~~~~~~~~~~~~~~~~~~~MEALRRIMKRLTRRLKMLKRLLKKFCWNKKIDK 

             121                                                      180
     RatL19  HM.YHSLYLKVKGNVFKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEARKRREER 
   HumanL19  HM.YHSLYLKVKGNVFKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEARKRREER 
   MouseL19  HM.YHSLYLKVKGNVFKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEARKRREER 
 DmelanoL19  HL.YHDLYMKCKGNVFKNKRVLMEYIHKKKAEKQRSKMLADQAEARRQKVREARKRREER 
     T2H3.3  HM.YHDMYMKVKGNVFKNKRVLMESIHKSKAEKAREKTLSDQFEAKRAKNKASRERKHAR 
   AthalL19  HM.SMTMYMS~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
   ZmaysL19  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
DdiscoidL19  HS.YRELYLKAKGNVFKNKRTLIEYIVKSKTEKLREKLIQDQAQARRNKNKSIKDRRAAK 
CelegansL19  HL.YHELYLRAKGNNFKNKKNLIEYIFKKKTENKRAKQLADQAQARRDKNKESRKRREER 
  SpombeL19  HL.YHTLYLEAKGNTFKHKRALIEHIQRAKAEANRTKLIQEQQDARRARAKAARQRRAKA 
ScerevisL19  HL.YHVLYKESKGNAFKHKRALVEHIIQAKADAQREKALNEEAEARRLKNRAARDRRAQR 
  MvanniL19  SS.YRKLYRMAKGGAFRSRNHMKLYMK.EHGILAE~~~~~~~~~~~~~~~~~~~~~~~~~ 
  MjannaL19  KV.YRKLYRMAKGGAFRSRSHLFLYMR.EHELLK~~~~~~~~~~~~~~~~~~~~~~~~~~ 
 MthermoL19  ST.YRKLYKMAKGGAFKSKSYMKTYAR.DHDMLR~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  AfulgiL19  RT.YRILYRKAKGGEFRSVAHLKIYVE.QMKR*~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
 PhorikoL19  KT.YRMLYIRAKGGQFKNKHQLYLFLE.EHGLLKK*~~~~~~~~~~~~~~~~~~~~~~~~ 
  HmarisL19  SQ.YRDLYDKAGGGEFDSVADLERYIDANHGDA~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  SacidoL19  HT.YRLLYKRAKGNYFKNLSDVKSYLRQMGHKV~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  AthalL19a  LVYYHDMFMKVKGKVYKNKCVLMESMHKSSRERKFSGSEMRLALVTIKSCFIKICSEPEG 

             181                           213
     RatL19  LQAKKEEIIKTLSKEEETKK*~~~~~~~~~~~~
   HumanL19  LQAKKEEIIKTLSKEEETKK*~~~~~~~~~~~~
   MouseL19  LQSKKEEIIKTLSKEEETKK*~~~~~~~~~~~~
 DmelanoL19  IATKKQELIALHAKEDEIAAKAATAGH*~~~~~
     T2H3.3  REERLAKGPGGDIPAAAPPAQTAEVPAKKSKK*
   AthalL19  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
   ZmaysL19  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
DdiscoidL19  SAAKEIVSSN*~~~~~~~~~~~~~~~~~~~~~~
CelegansL19  QVVKRAELLRKISQSEKVIAGK*~~~~~~~~~~
  SpombeL19  VEEKREQLYTAAEKIEE*~~~~~~~~~~~~~~~
ScerevisL19  VAEKRDALLKEDA~~~~~~~~~~~~~~~~~~~~
  MvanniL19  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  MjannaL19  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 MthermoL19  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  AfulgiL19  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 PhorikoL19  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  HmarisL19  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  SacidoL19  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  AthalL19a  EKTASAPQ~~~~~~~~~~~~~~~~~~~~~~~~~

written 11 Sep 98
Larry Parnell