T2H3 contains a 261-bp element found elsewhere in the genome. As the alignment below indicates, this element is conserved in clone T13D8 at 91.9% identity. The element is also contained in clone K3K7 and is partially duplicated in a tandem arrangement.

An alignment of T2H3 and T13D8 is presented.

                      .         .         .         .         .
 T2H3  33151 ....................GATTGCAATTGCGCTGGGGCTTGCAAAGAC 33180
                                     |  |      |      | || | | 
T13D8  64850 ACATTACGTATTCCAATTTCTGCGGATAGATTTTTTCTTACTTCATACAG 64801
                      .         .         .         .         .
 T2H3  33181 TATGTATTGTTAGACCATCTACATTGGTAATCTCTCAAGAGAGTTTTTCA 33230
              |   | | | ||||||||| |||||||| |||||||||||||||| |||
T13D8  64800 AACTAAATCTAAGACCATCTCCATTGGTAGTCTCTCAAGAGAGTTTCTCA 64751
                      .         .         .         .         .
 T2H3  33231 CTATTAAAATAACAGAAATAATAAAAAGAGA..AGAGAGAGTGTAGAAAG 33278
             ||| |||||||| | ||||||||||||||||  ||||||||| |||||||
T13D8  64750 CTACTAAAATAATAAAAATAATAAAAAGAGAAGAGAGAGAGTATAGAAAG 64701
                      .         .         .         .         .
 T2H3  33279 TTTCTCAAGTGAGAAACTATGAGATACTCTCTACAAGCCATCATTACATT 33328
             |||||||||||||||||||||||||| |||||||||||||||||||||||
T13D8  64700 TTTCTCAAGTGAGAAACTATGAGATATTCTCTACAAGCCATCATTACATT 64651
                      .         .         .         .         .
 T2H3  33329 TCTCTTCTTTTAATTGATTCAATTTTTACACTAAAATAAAAATTTAATCA 33378
             ||||| || | ||||| |||||||||| || |||||||||||||||||||
T13D8  64650 TCTCTCCTCTCAATTGGTTCAATTTTTTCATTAAAATAAAAATTTAATCA 64601
                      .         .         .         .         .
 T2H3  33379 TATAATAAAATTATAATAAAATACTAATATTTTTCTTGTTCGAGAAACTT 33428
             |||||||||| | || |||||||||||   |||  ||||| || ||||||
T13D8  64600 TATAATAAAAATTTAGTAAAATACTAA...TTTGTTTGTTTGAAAAACTT 64554
                      .         .         .         .         .
 T2H3  33429 TTTGGGTTTCTCCTTGATGGAGATACTCTTAGGTTTTGTTAAACTCTACA 33478
             |||||||||||||||||||||||| | |  || | || || |    |   
T13D8  64553 TTTGGGTTTCTCCTTGATGGAGATGCCCAAAGATATTTTTTACACTTCAT 64504
                      .         .    
 T2H3  33479 ACACATCAAGCTTAAACCTTTG.. 33500
             | |   | |  |   |  | |   
T13D8  64503 ATAGTACTAATTACTAAATCTAGG 64480

 

A comparison of T2H3 to K3K7 is summarized in the following table. It is interesting to note that segments 2 and 3 are contiguous in K3K7 and that T2H3 residues 33362 to 33374 appear as part of 3 distinct segments (1, 2 and 4) in K3K7. Different portions of the T2H3 repeat are noted in progressively downstream regions of K3K7.

Segment Range in T2H3 Range in K3K7 % identity Remark
1 33213 - 33464 59262 - 59513 90.1% full element comparable to the T13D8 alignment
2 33276 - 33374 59508 - 59606 85.9% 5' portion of repeat element
3 33407 - 33457 59607 - 59657 80.4% 3' portion of repeat element
4 33362 - 33388 59775 - 59801 85% small central portion of the element

 

written 10 Sep 98
Larry Parnell