T32N15.4 is most similar to hypothetical protein T32N15.3, encoded by BAC T32N15 from position 14523 to 17066, reverse strand. The termination codon of T32N15.4 lies just 729 bp upstream of the end of the Activator-like transposon. The 5' region of T32N15.4 is not defined. T32N15.4 exhibits moderate similarity to EST F14402, the 3' sequence of clone TAP0138.

CDS:				exon terminus predicted by	Gr=Grail
					3'		5'  	M=MZEF
	exon 1	32676 to 32890		Gr		Gr
	exon 2	32335 to 32380		Gr		Gr
	exon 3	31978 to 32130		Gr		Gr
	exon 4	30868 to 31170		Gr,M		Gr
	exon 5	30134 to 30586		Gr		Gr

GACTTCTCTGTCTCCTTCCACCAGGACTTCTGCTTCTCCTCTTACTCCTCCGTCCTCTCT
TGCGGCGAGGACTATTGCTCCCGTATCTTCTGCTCCTGCTACGGCTCTCCCCATCATCAT
GACCATACTTCTACTGCGGTTATGCCTTTCATGAGAGCGATCTTCGTGACTATAATGCCT
GTAAGGGCTCCTATACTTCCGCAATGGCTATGCCTGAGTGATAATATCGGGACGTTGATT
GATGATTTTGCTGCAGATCATAAATTGTTTGAGCCGGCCCTGCTGATACGTATTCGAAAG
GAGAAGCGTTGGGCTGATACTTGGTTTGGTGTAGAGACGAGAGCATCGATCTCCGTTACG
ACAAACGCCGATTTTGAAGTAGAAAGGACAATTCACGCGATCTTTCTCCGTTCCATCAGT
TCTATCTTTTCTTTGTCTATAGAAACGGAAACATGGTTTGACCAAAACGTTGTTCTCTCT
GGGAATCGACACATGATTTCCCTTACCAAACTTAATGCGAAGAAAGGTGGATTTCTTGTG
AACAATGAAGTCAAGATTGTTGTGGAGGTTGATGTTCTTCAAGTTATTGGCAAATTAGAT
GTTTCAGAGGGATCTCAAGAAGTAACTCAACCTCTGAAAAGGATAAGGCTAAACGATGAT
GGTGTTTCCGTAAAACAAAGCATTGATGTCAATGGGTTTCAGGTTCTTCCTTCACAGGTG
GAATCGGTGAAACGTATCTTTGAAAGACACCCAAACATGGCACTAGAGTTCCGAGCAAAG
AACCAACATGTGAGGACATCATGTATGAATGTCCTCCTCAGCCTAATCGATACACTGTGC
CAGTCACTGCAAGACATTTCCATTGATGACCTGGGCCAGGCAGAGAAAGCACTAAGATAC
CTGAAAGATTCGGACTTTAAGGTGGATTGGTTAGAGCGTAAATTGGAGGAAGTGAAGGAA
AAGAAGATGGAAGAGCAGATTGGTAAGTCTCGGATGCAAGAACTAGAGGAAGAGCTGAAG
ATCTTCAAGCAGAAGTGCTCTGACATAGAAGCTCAACTGGAGAAAGAGAAGCAAAAGTGC
TCAGACATAGAAGCTTTGCTGGAGAAAGAGAAGGCAAAGTCGTTGGCCGCTGCCAGAGCT
CCTCCTCTAACGTTGGATGATGTTGTTTGA


Protein translation:

DFSVSFHQDFCFSSYSSVLSCGEDYCSRIFCSCYGSPHHHDHTSTAVMPFMRAIFVTIMP
VRAPILPQWLCLSDNIGTLIDDFAADHKLFEPALLIRIRKEKRWADTWFGVETRASISVT
TNADFEVERTIHAIFLRSISSIFSLSIETETWFDQNVVLSGNRHMISLTKLNAKKGGFLV
NNEVKIVVEVDVLQVIGKLDVSEGSQEVTQPLKRIRLNDDGVSVKQSIDVNGFQVLPSQV
ESVKRIFERHPNMALEFRAKNQHVRTSCMNVLLSLIDTLCQSLQDISIDDLGQAEKALRY
LKDSDFKVDWLERKLEEVKEKKMEEQIGKSRMQELEEELKIFKQKCSDIEAQLEKEKQKC
SDIEALLEKEKAKSLAAARAPPLTLDDVV*


Alignment of T32N15.3, T32N15.4 and the conceptual translation of EST F14402:


T32N15.4       1 DFSVSFHQDFCFSSYSSVLSCGEDYCSRIFCSCYGSPHHHDHTSTAVMPF 50

T32N15.3       1 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MGYEKFTWVIKNFSSL 16
T32N15.4      51 MRAIFVTIMPVRAPILPQWLCLSDNIGTLIDDFAADHKLFEPALLIRIRK 100

T32N15.3      17 QSEYIKSDIFVIGGCKWRIRFRLTV.VNQLSENLSRRGGDFVFLWSTEGK 65
T32N15.4     101 EKRWADTWFGVETRASISVTTNADFEVERTIHAIFLRSISSIFSLSIETE 150

T32N15.3      66 RWFDKKLPLCGYEEVLLLTKLNAKHGGFLVNNEVKIVAEVDVLEVIGKLD 115
T32N15.4     151 TWFDQNVVLSGNRHMISLTKLNAKKGGFLVNNEVKIVVEVDVLQVIGKLD 200

T32N15.3     116 VSKESQEVIKPRKRMRLYGDGGAVSSHLHKETSSVDVNGFQVLPSQAESV 165
T32N15.4     201 VSEGSQEVTQPLKRIRLNDDGVSVK.......QSIDVNGFQVLPSQVESV 243

T32N15.3     166 KRIFERHPYMALEFRAKNQQLRASCINVLLSLIKTLCQSLQDISIDDLGQ 215
T32N15.4     244 KRIFERHPNMALEFRAKNQHVRTSCMNVLLSLIDTLCQSLQDISIDDLGQ 293
F14402                                                          LSD

T32N15.3     216 TEQVLTFLQNSGFKVDWLERKLEEVKEKKIQEHIGKSRMQGLEEDLKVFK 265
T32N15.4     294 AEKALRYLKDSDFKVDWLERKLEEVKEKKMEEQIGKSRMQELEEELKIFK 343
F14402           ADAALAYLTDAGLNLNWLEEKLEEVSEKKENEEAGETRVHEIEEELKELK

T32N15.3     266 KKCSDIEALLEKEKEELKGLKQKCSDIEALLEKEKGKVLAAAARTPLTLD 315
T32N15.4     344 QKCSDIEAQLEKE.......KQKCSDIEALLEKEKAKSLAAARAPPLTLD 386
F14402           LKCSNLEAQLEKE.......KADVS

T32N15.3     316 RMNILIGYREMISLTKLNAKKGGFLVNNKVKIVVEVDVLQVVGGLDVSQE 365
T32N15.4     387 DVV*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 390

click here to view a comparison of the nucleotide sequences encoding these genes

written 26 Aug 97
updated 28 Aug 97
Larry Parnell