Gene:   T32N15.8
Putative Identification:   histone deacetylase
Position:   60433 to 62392, from 5'-UTR through the CDS to the termination codon
Strand:   -
EST match:   N37851
Database match:   Zea mays nucleolar histone deacetylase HD2-p39 mRNA (U82815)

Note: T32N15.8 also has good database matches to A. thaliana chromosome V sequences MED24 (position 2245 to 3868) and MDJ22 (position 30864 to 32396). Because the MED24 and MDJ22 GenBank entries are not annotated, it is unclear if these chromosome V sequences encode putative histone deacetylases.

mRNA:

Based on a comparison to EST N37851, a 38-nucleotide putative 5'-UTR sequence
resides from 62355 to 62392.

62392  TAAAAATCCTCTCTTTTTCTCAACCTTGATTCTTAGCCatg  62352


CDS:				exon terminus predicted by	Gr=Grail
					3'		5'   	M=MZEF
	exon 1	62342 to 62354		EST,M
	exon 2	62037 to 62107		EST,M		EST,M
	exon 3	61727 to 61952		EST,M,Gr	EST,M,Gr
	exon 4	61585 to 61620		EST,M,Gr	EST,M,Gr
	exon 5	61420 to 61514		M,Gr		EST,M,Gr
	exon 6	61140 to 61293		M		M,Gr
	exon 7	60973 to 61051		M,Gr		M,Gr
	exon 8	60818 to 60877		M,Gr		M,Gr
	exon 9	60433 to 60472				Gr

ATGGAGTTCTGGGGAATTGAAGTTAAATCAGGAAAGCCAGTTACAGTGACTCCTGAAGAA
GGCATTCTTATCCACGTTTCTCAGGCATCGCTTGGAGAATGTAAAAACAAGAAGGGAGAG
TTTGTGCCTTTACATGTAAAGGTTGGGAACCAGAACTTGGTTCTGGGAACTCTATCGACT
GAGAACATCCCTCAGCTTTTCTGTGATTTGGTATTCGACAAGGAGTTTGAGCTTTCTCAC
ACTTGGGGAAAAGGAAGTGTTTACTTTGTTGGATACAAAACTCCCAACATTGAGCCACAA
GGCTATTCTGAGGAAGAAGAGGAAGAAGAGGAAGAAGTTCCTGCTGGGAATGCTGCCAAG
GCTGTAGCTAAACCAAAGGCTAAGCCTGCAGAAGTGAAGCCAGCTGTTGATGATGAAGAG
GATGAGTCTGATTCTGACGGAATGGATGAAGATGATTCTGATGGTGAGGATTCTGAGGAA
GAAGAGCCTACACCTAAGAAGCCTGCATCAAGCAAGAAGAGAGCTAATGAAACTACCCCT
AAAGCACCTGTGTCAGCAAAGAAGGCGAAAGTAGCAGTTACTCCTCAGAAAACAGATGAG
AAGAAGAAAGGGGGAAAGGCTGCAAACCAGAGCCCAAAGTCGGCCAGTCAAGTCTCATGT
GGTTCATGCAAGAAGACTTTCAACTCAGGGAATGCACTTGAGTCTCACAACAAGGCCAAG
CACGCTGCTGCCAATTTCTTCACTGCTCTTAATGCTAAGTGTATGATTGTATGA


Protein translation:

MEFWGIEVKSGKPVTVTPEEGILIHVSQASLGECKNKKGEFVPLHVKVGNQNLVLGTLST
ENIPQLFCDLVFDKEFELSHTWGKGSVYFVGYKTPNIEPQGYSEEEEEEEEEVPAGNAAK
AVAKPKAKPAEVKPAVDDEEDESDSDGMDEDDSDGEDSEEEEPTPKKPASSKKRANETTP
KAPVSAKKAKVAVTPQKTDEKKKGGKAANQSPKSASQVSCGSCKKTFNSGNALESHNKAK
HAAANFFTALNAKCMIV*


Protein motifs:

T32N15.8 contains a putative C2H2 type zinc finger domain from residues 220 
to 241, highlighted below. HD2-p39 from Z. mays does not encode this motif.


Alignment of T32N15.8 (top) and ZmHD2-p39 (bottom)

                  .         .         .         .         .
       1 MEFWGIEVKSGKPVTVTPEEGILIHVSQASLGECKNKKGEFVPLHVKVGN 50
         |||||:||| |  |   |  | .:|.|||.|||  .|| :   ::||: .
       1 MEFWGLEVKPGSTVKCEPGYGFVLHLSQAALGE..SKKSDNALMYVKIDD 48
                  .         .         .         .         .
      51 QNLVLGTLSTENIPQLFCDLVFDKEFELSHTWGKGSVYFVGYKTP...NI 97
         | | :|||| :  | :  ||:||||||||||    ||:| |||       
      49 QKLAIGTLSVDKNPHIQFDLIFDKEFELSHTSKTTSVFFTGYKVEQPFEE 98
                  .         .         .         .         .
      98 EPQGYSEEEEEEEEEVPAGNAAKAVAKPKAKPAEVKPAVDDEEDESDSDG 147
         :      |:|:||  ||         . | |  |   |   .        
      99 DEMDLDSEDEDEELNVPVVKENGKADEKKQKSQEKAVAAPSKSSPDSKKS 148
                  .         .         .         .         .
     148 MDEDDSDGEDS....EEEEPTPKKPASSKKRANETTPKAPVSAKKAKVAV 193
          |:|||| ::.    |:|     .  ||..  .:.. .   |  . .   
     149 KDDDDSDEDETDDSDEDETDDSDEGLSSEEGDDDSSDEDDTSDDEEEDTP 198
                  .         .         .         .         .
     194 TPQKTD..EKKKGGKAANQSPKSASQVSCGSCKKTFNSGNALESHNKAKH 241
         ||.| :  .|:    .  ..| |  .    .       |    . . |  
     199 TPKKPEVGKKRPAESSVLKTPLSDKKAKVATPSSQKTGGKKGAAVHVATP 248
                  .         .         .         .         .
     242 AAANFFTALNAKCMIV*........................................... 257
           |   | .|    :                                   
     249 HPAKGKTIVNNDKSVKSPKSAPKSGGSVPCKPCSKSFISETALQAHSRAKMGASESQVQ* 307

created 4 Sep 97
Larry Parnell