Gene T3F12.1
Putative Identification phosphoglyceride transfer protein
Position 12749 to 14160, from within the CDS to the termination codon
Strand +
EST match none
Database match Z99708, A. thaliana chromosome IV sequence (contig AP2)

 

Note:  The 5' region of gene T3F12.1 is not defined.

CDS:  The table below lists the coordinates for each exon and which gene prediction algorithms predicted the 5' and 3' termini of a particular exon (GS = GenScan, Gr = Grail, M = MZEF).

Exon Range 5' 3'
1 12749 to 12803 M M
2 12954 to 13033 M,GS M,Gr,GS
3 13100 to 13276 M,Gr M,Gr
4 13351 to 13449 M,Gr,GS M,Gr,GS
5 13527 to 13769 M,Gr,GS M,Gr,GS
6 13840 to 14160 Gr,GS GS

CDS of T3F12.1 from within the CDS to the termination codon

ACAAAGATCCTCCCGACCAAACATTTTGCTTCCGTGAAAAACGATTGTGGTCTCTGGTTT
AAAGAAGTTATGAACACTAATCCAGTGACTAATGGGTTTGTTAAACCTGTTCCTACTGAA
GAAGAACAAGCAAAGATTGAGGAAGTGAGGAAGCTATTAGGTCCATTACCGGAGAAGCTT
TCGAGTTTTTGTTCCGATGATGCAGTTTTAAGGTATCTAAGGGCAAGGAATTGGCATGTC
AAGAAAGCTACTAAGATGCTTAAAGAAACTTTGAAATGGAGAGTTCAATACAAACCTGAG
GAGATTTGTTGGGAGGAAGTAGCTGGTGAAGCAGAGACAGGGAAGATATATAGATCGAGT
TGTGTCGATAAACTTGGACGACCTGTTCTCATTATGAGACCGAGTGTTGAGAATTCTAAA
TCGGTGAAAGGCCAGATTAGATACCTTGTGTATTGTATGGAGAATGCAGTACAAAATTTG
CCACCAGGGGAAGAACAGATGGTGTGGATGATAGATTTTCACGGTTATAGTTTGGCGAAT
GTATCGTTACGAACAACGAAAGAAACCGCTCATGTATTACAAGAACATTACCCTGAGAGA
TTAGCTTTTGCTGTTCTCTACAATCCTCCTAAGTTCTTTGAACCTTTCTGGAAGGTGGCG
CGGCCCTTCTTAGAGCCGAAGACACGGAACAAAGTGAAGTTTGTTTACTCGGATGATCCA
AACACTAAGGTGATAATGGAGGAGAACTTTGATATGGAGAAAATGGAGTTAGCGTTTGGT
GGTAACGATGACTCGGGTTTTAACATAGAGAAACATTCAGAGAGAATGAAAGAGGACGAC
AAGAAAAGATTGGCTTCGTTGGAGGGCATTGTTTCCGCTTCTCTAGACTCGCTCAGCATT
TTATCGGTCTCTGATGGTACCGCCTCTAACAGTGCTCACCCTAGCTCTCATGATGTCTCT
GAGGATGAGCATTAA

 

Protein sequence: 

TKILPTKHFASVKNDCGLWFKEVMNTNPVTNGFVKPVPTEEEQAKIEEVRKLLGPLPEKL
SSFCSDDAVLRYLRARNWHVKKATKMLKETLKWRVQYKPEEICWEEVAGEAETGKIYRSS
CVDKLGRPVLIMRPSVENSKSVKGQIRYLVYCMENAVQNLPPGEEQMVWMIDFHGYSLAN
VSLRTTKETAHVLQEHYPERLAFAVLYNPPKFFEPFWKVARPFLEPKTRNKVKFVYSDDP
NTKVIMEENFDMEKMELAFGGNDDSGFNIEKHSERMKEDDKKRLASLEGIVSASLDSLSI
LSVSDGTASNSAHPSSHDVSEDEH*

Alignment of SEC14 and SEC14-like proteins from D. discoideum (DdRSC5, U82515), S. cerevisiae (YNL231c, Z71507), Rice (RiceSEC14-like, U88068), and Yarrowia lipolytica (YlipoSEC14, P45816) to A. thaliana sequences T3F12.1 and the AP2 hypothetical protein (from chromosome IV, Z99708). SEC14 proteins catalyze the transfer of phosphatidylinositol/phosphatidylcholine between membranes and are required for the transport of secretory proteins from the Golgi complex. The T3F12.1 protein is likely a phosphoglyceride transfer protein of uncharacterized specificity.


                 1                                                         60
        T3F12.1  TKILPTKHFASVKNDCGLWFKE...........VMNTNP....VTNGFVKPVP..TEEE. 
         DdRSC5  MESTASTSDSASAITTGLSEVD...........INQQSPPIILKEGEIFDPLKNLNEKQ. 
        YNL231c  MFKRFSKKKEAPEDPKNLINIDKPIKELPASIAIPKEKPLTGEQQKMYDEVLKHFSNPD. 
 RiceSEC14-like  ~~~~~~~~~~MAGNRGGDAGEGEWLKVAELRATVEAQDPHAKEVDNL............. 
AP2hypothetical  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MIQETLKWRSTYK. 
     YlipoSEC14  MTVTEQEFLASYPQKVAPGGPTGYPGNLTAEQEQKLGELKMILLTKGYEDRTDDATLLRF 

                 61                                                       120
        T3F12.1  QAKIEEVRKLLGPLPEKL....SSFCSDDAVLRYLRARNWHVKK................ 
         DdRSC5  LEAFKEIKSNFSDLTDPT...DIAFCTDMCFLRYLRARNYIVSK................ 
        YNL231c  LKVYTSEKNKSEDDLKPLEEEEKAWLTRECFLRYLRATKWVLKD................ 
 RiceSEC14-like  .............................TLRRFLRARDHNVEK................ 
AP2hypothetical  PQEIRWNQVAHEGETGKASRASFHDRQGRVVL.IMRPAMQVTKP................ 
     YlipoSEC14  LRARKFDVPLAQEMWENCEKWRKEFGTNTILEDFWYKEKKEVAKLYPQYYHKTDKDGRPV 

                 121                                                      180
        T3F12.1  .................ATKMLKETLKWRVQY.................KPEEICWEEVA 
         DdRSC5  .................SEKMLRDTLEWRKKF.................RPQDIQLGGDI 
        YNL231c  .................CIDRITMTLAWRREFGISHLGEE.........HGDKITADLVA 
 RiceSEC14-like  .................ASAMLLKALRWRREAVPGGSVPE.........EKVQSDLDDD. 
AP2hypothetical  .................IVTFMNIFLEFFLKM............................ 
     YlipoSEC14  YVENVGKVNIHEMYKITTQERMLRNLVWEYESFVRHRLPACSRVVGHLIETSCTILDLKG 

                 181                                                      240
        T3F12.1  GEAETGKIYRSSCVDKLGRPVLIMRPSVENSKSVKGQIRY..LVYCMENAVQNL..PP.. 
         DdRSC5  REIGSAGCVYVNKRDKKGRPIIFAVPRNDTLKNVPSELKFKNLVYWLEQGFSRMDEPK.. 
        YNL231c  VENESGKQVILG.YENDARPILYLKPGRQNTKTSHRQVQ..HLVFMLERVIDFM..PA.. 
 RiceSEC14-like  .......KVYMGGADRTGRPILLGFPVKNFSAKRDMPKFKSYCVYLLDSICARI..PR.. 
AP2hypothetical  ....................ISHLIKMKQNSTSQEGNIR..HLVYLLENAI..INLPK.. 
     YlipoSEC14  VSLSSASQVYGFLKDASNIGQNYYPERMGKFYLINAPFGFSTVFSVIKRFLDPVTVSKIH 

                 241                                                      300
        T3F12.1  ..GEEQMVWMIDFHGY........SLANVSLRTTKETAHVLQEHY...PERLAFAVLYNP 
         DdRSC5  ..GIEQFCFIVDYKDF........GSGNMDMKTNLEAMHFLLDHC...PERMGQSLFLDP 
        YNL231c  ..GQDSLALLIDFKDYPDVPKVPGNSKIPPIGVGKEVLHILQTHY...PERLGKALLTNI 
 RiceSEC14-like  ..GQEKFVCIVDLKGW........GYSNCDIRAYIAAIEIMQNYY...PERLGKALMIHV 
AP2hypothetical  ..GQKQMSWLIDFTGWSMAV.......NPPMKTTREIIHILQNYY...PERLGIAFLYNP 
     YlipoSEC14  VYGSNYKEKLLAQVPAYNLPIKFGGQSSSKIGVELSDDGPWRDPQFVGPEAWPPWLVSDP 

                 301                                                      360
        T3F12.1  PKFFEPFWKV...............ARPFLEPKTRNKVKFVYS................. 
         DdRSC5  PALFWFAWKI...............ISPFLNEVTLSKVRFINSKK............... 
        YNL231c  PWLAWTFLKL...............IHPFIDPLTREKLVF.................... 
 RiceSEC14-like  PYMFMKAWKM...............IYPFIDNVTRDKFVFV................... 
AP2hypothetical  PRLFQAVYRVHKKNIRHYYKTIKMAAKYFLDPRTAEKVKFVYPK................ 
     YlipoSEC14  PVPPPSSPTPPPTPSPLLPPRSVLTTRPLTQRPTVTELLLPLPELELLLLAVLLLLLELL 

                 361                                                      420
        T3F12.1  ......................................DDPNTKVIMEENFDMEKMELAF 
         DdRSC5  .....................................VDGKRTFAELLEYVDIENLEQNL 
        YNL231c  ......................................DEP.....FVKYVPKNELDSLY 
 RiceSEC14-like  ......................................DDKSLQEVLHQEIDDSQIPDTL 
AP2hypothetical  .......................................DKASDELMTTHFDVENLPKEF 
     YlipoSEC14  LPSRLLLPRLLLPRRQGSRSRCCQDRHCPCPHRQDCCTSDQLFPPEMLAMMEQNEKKAEK 

                 421                                                      480
        T3F12.1  GGNDDSGFNIEKHSERMKEDDKKRLASLEGIVSASLDSLSILSVSDGTASNSAHPSSHDV
         DdRSC5  GGNLDYNYNID...EYLKENP...........DPIVDTPPITFSSCRIFFQFILLIYFIN
        YNL231c  GGDLKFKYNHDVYWPALVETAREKRDHYFKRFQSFGGIVGLSEVDLRGTHEKLL..YPVK
 RiceSEC14-like  GGKL........APVSLKNNARINLD*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
AP2hypothetical  GGEATLEYDHEDFSRQMYEDDLKTAKYWGLEGKHYPKTNGFSPSD.......VVPEPAIE
     YlipoSEC14  AAEAKTKGGAAPQANAFTDEQLAQLNSLPQQVATGGVSQPLPDSHVTQGVPTVAKIAELP 

                 481            498
        T3F12.1  SEDEH*~~~~~~~~~~~~
         DdRSC5  LIDFI*~~~~~~~~~~~~
        YNL231c  SESSTV*~~~~~~~~~~~
 RiceSEC14-like  ~~~~~~~~~~~~~~~~~~
AP2hypothetical  IASAAS*~~~~~~~~~~~
     YlipoSEC14  PVPMRMPIFPIKTKKHT*

 

created 6 Oct 97
Larry Parnell