CDS:  The table below lists the 12 exons of T3F12.7, their coordinates within the BAC, and which exon-finding program(s) predicted the 5' or 3' terminus of that exon (GS = GenScan, Gr = Grail, M = MZEF). Exon 4 was selected on the basis of sequence homology. The intron between exons 11 and 12 is large, at 1871 nt, but exon 12 does encode sequence of good similarity to other plant sulfate transport proteins (see alignment below).

CDS from initial methionine to the termination codon:

ATGTCCGGGACTATTAATCCCCCGGACGGAGGAGGGTCCGGTGCAAGAAACCCACCAGTC
GTTCGTCAGAGAGTGCTAGCTCCTCCGAAAGCGGGTTTACTAAAGGACATCAAGTCCGTG
GTTGAAGAAACTTTCTTCCATGATGCTCCGCTTAGGGATTTCAAGGGCCAAACCCCAGCT
AAAAAAGCGTTGCTCGGGATCCAGGCTGTCTTCCCGATCATCGGGTGGGCCAGAGAATAC
ACTCTTCGCAAATTTAGAGGTGATCTCATCGCCGGTCTCACCATTGCTAGTCTTTGTATC
CCTCAGGATATCGGATATGCAAAACTCGCGAATGTCGATCCGAAATACGGACTTTATTCG
AGTTTCGTGCCACCGCTGATTTACGCGGGCATGGGGAGTTCTAGGGATATTGCGATAGGA
CCAGTCGCTGTGGTGTCTCTTCTTGTGGGAACTTTGTGCCAGGCCGTGATCGACCCAAAG
AAAAACCCGGAGGATTATCTCCGACTTGTCTTCACTGCCACTTTCTTTGCTGGCATTTTC
CAAGCCGGCCTCGGATTTCTACGGTTGGGATTCTTGATAGACTTTCTGTCGCATGCGGCC
GTGGTTGGGTTCATGGGAGGAGCAGCCATCACAATCGCTCTCCAACAGCTTAAGGGCTTT
CTTGGCATCAAAACATTTACCAAGAAAACTGATATTGTTTCTGTCATGCACTCCGTATTC
AAAAACGCTGAGCATGGGTGGAATTGGCAAACTATAGTCATTGGCGCCAGTTTCTTGACC
TTTCTTCTCGTCACCAAATTCATTGGGAAGAGAAACAGGAAACTATTTTGGGTTCCGGCA
ATTGCGCCTCTTATTTCAGTCATTATCTCTACCTTCTTTGTCTTCATTTTTCGTGCTGAT
AAACAAGGAGTCCAAATTGTGAAACATATAGATCAAGGAATCAATCCGATTTCCGTTCAT
AAGATTTTCTTCTCCGGAAAATATTTCACCGAAGGAATCCGAATCGGAGGCATTGCGGGT
ATGGTCGCCTTAACGGAGGCTGTAGCGATTGCAAGAACATTTGCGGCAATGAAAGACTAT
CAAATTGATGGAAACAAAGAGATGATTGCCCTAGGGACTATGAACGTCGTCGGTTCAATG
ACCTCTTGTTACATTGCCACGGGTTCGTTTTCGCGATCTGCCGTGAACTTCATGGCGGGA
GTCGAAACGGCGGTTTCAAACATAGTTATGGCCATAGTTGTAGCTCTAACCTTAGAGTTC
ATCACACCACTCTTCAAGTACACTCCAAATGCTATCCTCGCGGCCATCATTATATCGGCT
GTCCTCGGTCTTATCGATATTGACGCAGCGATTCTCATATGGAGGATCGATAAACTCGAC
TTCTTGGCTTGCATGGGAGCTTTCTTAGGAGTCATCTTCATCTCGGTTGAGATCGGTCTC
TTGATCGCTGTGGTGATCTCTTTTGCAAAGATATTGCTTCAAGTGACGAGACCAAGAACC
ACGGTTCTAGGGAAGCTGCCAAATTCGAATGTATATCGGAACACTCTACAGTATCCGGAC
GCTGCCCAAATTCCCGGAATCTTGATCATCCGTGTTGACTCGGCCATCTACTTTTCCAAC
TCCAACTATGTCCGAGAAAGGGCATCAAGATGGGTGCGAGAGGAGCAAGAAAATGCTAAG
GAATATGGCATGCCGGCAATCAGATTTGTGATTATTGAGATGTCACCGGTTACCGATATC
GATACCAGTGGTATCCACTCCATCGAAGAACTTCTCAAGAGCCTCGAGAAGCAAGAAATT
CAGTTGATTCTAGCAAATCCAGGACCAGTGGTGATTGAGAAACTTTATGCTTCAAAGTTC
GTCGAGGAGATTGGAGAGAAAAATATCTTCCTTACTGTTGGCGACGCGGTCGCAGTTTGT
TCTACGGAAGTGGCTGAGCAACAAACTTAA

Protein sequence:

MSGTINPPDGGGSGARNPPVVRQRVLAPPKAGLLKDIKSVVEETFFHDAPLRDFKGQTPA
KKALLGIQAVFPIIGWAREYTLRKFRGDLIAGLTIASLCIPQDIGYAKLANVDPKYGLYS
SFVPPLIYAGMGSSRDIAIGPVAVVSLLVGTLCQAVIDPKKNPEDYLRLVFTATFFAGIF
QAGLGFLRLGFLIDFLSHAAVVGFMGGAAITIALQQLKGFLGIKTFTKKTDIVSVMHSVF
KNAEHGWNWQTIVIGASFLTFLLVTKFIGKRNRKLFWVPAIAPLISVIISTFFVFIFRAD
KQGVQIVKHIDQGINPISVHKIFFSGKYFTEGIRIGGIAGMVALTEAVAIARTFAAMKDY
QIDGNKEMIALGTMNVVGSMTSCYIATGSFSRSAVNFMAGVETAVSNIVMAIVVALTLEF
ITPLFKYTPNAILAAIIISAVLGLIDIDAAILIWRIDKLDFLACMGAFLGVIFISVEIGL
LIAVVISFAKILLQVTRPRTTVLGKLPNSNVYRNTLQYPDAAQIPGILIIRVDSAIYFSN
SNYVRERASRWVREEQENAKEYGMPAIRFVIIEMSPVTDIDTSGIHSIEELLKSLEKQEI
QLILANPGPVVIEKLYASKFVEEIGEKNIFLTVGDAVAVCSTEVAEQQT*

Alignment of T3F12.7, Stylosanthes hamata sulfate transporters 1 (ST1) and 2 (ST2), and barley ST1. The peptide encoded by T3F12.7 exon 4, which was not selected by any of the gene prediction programs, is highlighted in red. The peptide encoded by T3F12.7 exon 12 is underlined. Many different sulfate transporter genes have been sequenced from a variety of plant sources; only the most similar sequences are aligned here. Visit the Sulfate Transport page for a more extensive overview and alignment of sulfate transporter proteins.

            1                                                         60
ShamataST1  MSQRVSDQVMADVIAETRSNSSSHRHGGGGGGDDTTSLPYMHKVGTPPKQTLFQEIKHSF 
ShamataST2  MSQRVSDQAMAEVIAETRTNSSSRRH...GGGDDTPSLPYMHKVGAPPKQTLFQEIKHSF 
 BarleyST1  MPRTVSDGG.EDFDGDVCSQTASQRH......TDSTHHHHGYKVGFPPAKGVFAEFAEGV 
   T3F12.7  ~~~~~~~~~~~~~~~~~~~MSGTINPPDGGGSGARNPPVVRQRVLAPPKAGLLKDIKSVV 

            61                                                       120
ShamataST1  NETFFPDKPFGKFKDQSGFRKLELGLQYIFPILEWGRHYDLKKFRGDFIAGLTIASLCIP 
ShamataST2  NETFFPDKPFGNFKDQSGSRKFVLGLQYIFPILEWGRHYDLKKFRGDFIAGLTIASLCIP 
 BarleyST1  KETFFADDPLREYKDQPRSKKLWLSLVHLFPVLDWSRSYTFGKFKGDLVAGLTIASLCIP 
   T3F12.7  EETFFHDAPLRDFKGQTPAKKALLGIQAVFPIIGWAREYTLRKFRGDLIAGLTIASLCIP 

            121                                                      180
ShamataST1  QDLAYAKLANLDPWYGLYSSFVAPLVYAFMGTSRDIAIGPVAVVSLLLGTLLSNEISNTK 
ShamataST2  QDLAYAKLANLDPWYGLYSSFVAPLVYAFMGTSRDIAIGPVAVVSLLLGTLLSNEISNTK 
 BarleyST1  QDIGYAKLANLQPHVGLYSSFVPPLIYALMGSSRDIAIGPVAVVSLLLGTLLQEEIDPVK 
   T3F12.7  QDIGYAKLANVDPKYGLYSSFVPPLIYAGMGSSRDIAIGPVAVVSLLVGTLCQAVIDPKK 

            181                                                      240
ShamataST1  SH.DYLRLAFTATFFAGVTQMLLGVCRLGFLIDFLSHAAIVGFMAGAAITIGLQQLKGLL 
ShamataST2  SH.DYLRLAFTATFFAGVTQMLLGVCRLGFLIDFLSHAAIVGFMAGAAITIGLQQLKGLL 
 BarleyST1  NPLEYSRLAFTATFFAGITQAMLGFFRLGFIIEFLSHAAIVGFMAGAAITIALQQLKGLL 
   T3F12.7  NPEDYLRLVFTATFFAGIFQAGLGFLRLGFLIDFLSHAAVVGFMGGAAITIALQQLKGFL 

            241                                                      300
ShamataST1  GISNNNFTKKTDIISVMRSVWTHVHHGWNWETILIGLSFLIFLLITKYIAKKNKKLFWVS 
ShamataST2  GI..KDFTKNSDIVSVMHSVWSNVHHGWNWETILIGLSFLIFLLITKYIAKKNKKLFWVS 
 BarleyST1  GIAK..FTKKSDIISVMESVWGNVQHGWNWQTILIGSSFLAFLLTTKYIAKKNKKLFWVS 
   T3F12.7  GI..KTFTKKTDIVSVMHSVFKNAEHGWNWQTIVIGASFLTFLLVTKFIGKRNRKLFWVP 

            301                                                      360
ShamataST1  AISPMISVIVSTFFVYITRADKRGVSIVKHIKSGVNPSSANEIFFHGKYLGAGVRVGVVA 
ShamataST2  AISPMICVIVSTFFVYITRADKRGVTIVKHIKSGVNPSSANEIFFHGKYLGAGVRVGVVA 
 BarleyST1  AIAPLISVVISTFCVYITRADKQGVAIVKNIKQGINPPSFDLIYWSGPYLAKGFRIGVVS 
   T3F12.7  AIAPLISVIISTFFVFIFRADKQGVQIVKHIDQGINPISVHKIFFSGKYFTEGIRIGGIA 

            361                                                      420
ShamataST1  GLVALTEAIAIGRTFAAMKDYALDGNKEMVAMGTMNIVGSLSSCYVTTGSFSRSAVNYMA 
ShamataST2  GLVALTEAMAIGRTFAAMKDYSIDGNKEMVAMGTMNIVGSLTSCYVTTGSFSRSAVNYMA 
 BarleyST1  GMVALTEAIAIGRTFAAMKDYQIDGNKEMVALGTMNIVGSMTSCYVATGSFSRSAVNYMA 
   T3F12.7  GMVALTEAVAIARTFAAMKDYQIDGNKEMIALGTMNVVGSMTSCYIATGSFSRSAVNFMA 

            421                                                      480
ShamataST1  GCKTAVSNIVMSIVVLLTLLVITPLFKYTPNAVLASIIIAAVVNLVNIEAMVLLWKIDKF 
ShamataST2  GCKTAVSNIVMAIVVLLTLLVITPLFKYTPNAVLASIIIAAVVNLVNIEAMVLLWKIDKF 
 BarleyST1  GCKTAVSNVVMAIVVMLTLLLITPLFKYTPNAILASIIINAVVNLVDYETAYLIWKVDKM 
   T3F12.7  GVETAVSNIVMAIVVALTLEFITPLFKYTPNAILAAIIISAVLGLIDIDAAILIWRIDKL 

            481                                                      540
ShamataST1  DFVACMGAFFGVIFKSVEIGLLIAVAISFAKILLQVTRPRTAVLGKLPGTSVYRNIQQYP 
ShamataST2  DFVACMGAFFGVIFKSVEIGLLIAVAISFAKILLQVTRPRTAVLGKLPGTSVYRNIQQYP 
 BarleyST1  DFVALLGAFFGVVFASVEYGLLIAVAISLGKILLQVTRPRTALLGNLPRTTIYRNVEQYP 
   T3F12.7  DFLACMGAFLGVIFISVEIGLLIAVVISFAKILLQVTRPRTTVLGKLPNSNVYRNTLQYP 

            541                                                      600
ShamataST1  KAAQIPGMLIIRVDSAIYFSNSNYIKERILRWLIDEGAQRTESELPEIQHLITEMSPVPD 
ShamataST2  KAEQIPGMLIIRVDSAIYFSNSNYIKERILRWLIDEGAQRTESELPEIQHLIVEMSPVTD 
 BarleyST1  EATKVPGVMIVRVDSAIYFTNSNYVKERILRWLRDEEEQQQEQKLSKTEFLIVELSPVTD 
   T3F12.7  DAAQIPGILIIRVDSAIYFSNSNYVRERASRWVREEQENAKEYGMPAIRFVIIEMSPVTD 

            601                                                      660
ShamataST1  IDTSGIHAFEELYKTLQKREVQLILANPGPVVIEKLHASKLTELIGEDKIFLTVADAVAT 
ShamataST2  IDTSGIHAFEELYKTLQKREVQLMLANPGPVVIEKLHASNLAELIGEDKIFLTVADAVAT 
 BarleyST1  IDTSGIHALEELLKALEKRKIQLILANPGPAVIQKLRSAKFTDLIGDDKIFLSVGDAVKK 
   T3F12.7  IDTSGIHSIEELLKSLEKQEIQLILANPGPVVIEKLYASKFVEEIGEKNIFLTVGDAVAV

            661     671
ShamataST1  YGPKTAAF*~~
ShamataST2  YGPKTAAF*~~
 BarleyST1  FAPKSSLNV*~
   T3F12.7  CSTEVAEQQT*

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created 6 Oct 97
Larry Parnell